Patterns of Expansion and Expression Divergence of the Polygalacturonase Gene Family in Brassica oleracea

Lyu, Meiling; Iftikhar, Junaid; Guo, R. S.; Wu, Binghua; Cao, Jiashu

doi:10.3390/ijms21165706

Cited by 11 publications

(10 citation statements)

References 52 publications

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“…Most IbPG proteins in our analysis possessed domain I and II, suggesting that these proteins may still be catalytically active but lost the ability to engage with the substrate that contain the ionic groups of the carboxylic acid groups. The members of Clade E were de cient in the domain III, and the similar results were observed in B. oleracea [33]. According to speculation, the members of Clade A and B include endo-PGs, the members of Clade C and D contain exo-PGs, Clade E members contain rhamno PGs, and Clade F members are neither exo-PGs nor endo-PGs, the variations in pectin components that each clade catalyzed may be shown by the evolutionary distinctions because of different types of PG enzyme have different substrates and products.…”

Section: Discussionsupporting

confidence: 77%

“…The PG genes had been identi ed in many advanced plants recently [24]. For instance, there were 68, 46, 99, 75, 53, 62 PGs had been reported in A. thaliana, Oryza sativa, Brassica oleracea, Populus, Cucumis sativus and Citrullus lanatus, respectively [12,13,26,28,33]. The expression of PG genes played a vital role in alterations and modi cations of plant cell walls.…”

Section: Discussionmentioning

confidence: 99%

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Genome-Wide identification and expression analysis of the polygalacturonase gene family in sweetpotato

Zhang

et al. 2023

Preprint

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Background: Polygalacturonase (PG), a crucial enzyme involved in pectin degradation, is associated with various plants' developmental and physiological processes such as seed germination, fruit ripening, fruit softening and plant organ abscission. However, the members of PG gene family in sweetpotato (Ipomoea batatas) have not been extensively identiﬁed. Results:In this study, there were 103 PG genes identified in sweetpotato genome, which were phylogenetically clustered into divergent six clades. The gene structure characteristics of each clade were basically conserved. Subsequently, we renamed these PGs according to their locations of the chromosomes. The investigation of collinearity between the PGs in sweetpotato and other four species, contained Arabidopsis thaliana, Solanum lycopersicum, Malus domestica and Ziziphus jujuba, revealed important clues about the potential evolution of the PG family in sweetpotato. Gene duplication analysis showed that IbPGs with collinearity relationships were all derived from segmental duplications, and these genes were under purifying selection. In addition, each promoter regionof IbPG proteins contained cis-acting elements related to plant growth and development processes, environmental stress responses and hormone responses. Furthermore, the 103 IbPGs were differentially expressed in various tissues (leaf, stem, proximal end, distal end, root body, root stock, initiative storage root and fiber root) and under different abiotic stresses (salt, drought, cold, SA, MeJa and ABA treatment). IbPG038 and IbPG039were down-regulated with salt, SA and MeJa treatment. According to the further investigation, we found that IbPG006, IbPG034 and IbPG099had different patterns under the drought and salt stress in fiber rootof sweetpotato, which provided insights into functional differences among these genes. Conclusion: A total of 103 IbPGs were identified and classified into six clades from sweetpotato genome. The results of RNA-Seq and qRT-PCR suggested that IbPG006, IbPG034 and IbPG099 might play a significant role in tissue specificity as well as drought and salt stress responses, which showed valuable information for further functional characterization and application of the IbPGs.

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Section: Discussionsupporting

confidence: 77%

Section: Discussionmentioning

confidence: 99%

Genome-Wide identification and expression analysis of the polygalacturonase gene family in sweetpotato

Zhang

et al. 2023

Preprint

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“…What is important, they included cis-elements regulated by phytohormones and transcription factors, such as abscisic acid (ABRE), gibberellin (P-Box), auxin (TGA-element), methyl jasmonate (CGTCA, TGACG), and MYB binding site involved in drought-inducibility (MBS). Similar research was carried out in Brassica oleracea, and the hormone-response cis-elements were also found for MeJA (CGTCA), auxin (TGA-box and AuxRR-core), GA (GARE, P-box, TATC-box), salicylic acid (TCA-element), and abscisic acid (ABRE) [63].…”

Section: Discussionsupporting

confidence: 71%

Anther Dehiscence Is Regulated By Gibberellic Acid In Yellow Lupine (Lupinus Luteus L.)

Marciniak

Przedniczek

2021

Preprint

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Background:Anther dehiscence resulting in the release of pollen grains is tightly regulated in spatio-temporal manner by various factors. In yellow lupine (Lupinus luteus L.), the species which shows cleistogamy, the anthers split before the flowers open but the course and regulation of this process is unknown. Specific control of the anther development takes place with the participation of hormonal pathways, the wide action of which ensures reproductive success. In our previous research we have shown that the lowest transcript level of LlDELLA1, a main repressor of gibberellin (GA) signaling, in flower and early pod development occurs about the time of anther opening; therefore the main purpose of this study was to precisely investigate the gibberellic acid (GA3)-dependent regulation of anther dehiscence program in yellow lupine.Results:In this paper we showed the specific changes in the yellow lupine anther structure during the dehiscence and identified several genes widely-associated with this process, including deposition of lignocellulosic secondary thickening in the endothecium, enzymatic breakdown of cell walls at the septum/stomium and cell degeneration via programmed cell death (PCD). The expression profile of genes varied over time with the most intense mRNA accumulation in the phases prior to or about time of anther opening. The transcriptional activity revealed also that these genes are highly co-expressed and regulated in a GA-dependent manner. The cellular and tissue localization of GA3 exhibited that these molecules are present right before anther opening, mainly in septum cells, near the vascular bundle and in the endothecium, and later they were completely undetectable. The GA3 localization strongly correlates with the transcriptional activity of genes related with GA biosynthesis and deactivation. The obtained results also suggest that GA3 controls LlGAMYB expression via LlMIR159-dependent pathway.Conclusions:The presented results show a clear contribution of GA3 in the control of the extensive anther dehiscence program in yellow lupine. Understanding the processes underlying pollen release at hormonal and molecular level is a significant aspect in controlling fertility in economically important legume crop species and increased attention of breeders.

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“…Importantly, they included cis-elements regulated by phytohormones and transcription factors such as abscisic acid (ABA; ABRE), GAs (P-Box), auxin (TGA-element), methyl jasmonate (MeJA; CGTCA, TGACG), and MYB binding sites involved in drought inducibility (MBS). Similar research was carried out in Brassica oleracea , and hormone-response cis-elements were found for MeJA (CGTCA), auxin (TGA-box and AuxRR-core), GA (GARE, P-box, TATC-box), salicylic acid (TCA-element), and ABA (ABRE) [ 69 ].…”

Section: Discussionmentioning

confidence: 59%

Anther dehiscence is regulated by gibberellic acid in yellow lupine (Lupinus luteus L.)

Marciniak

Przedniczek

2021

BMC Plant Biol

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Background Anther dehiscence resulting in the release of pollen grains is tightly regulated in a spatiotemporal manner by various factors. In yellow lupine (Lupinus luteus L.), a species that shows cleistogamy, the anthers split before the flowers open, but the course and regulation of this process are unknown. The specific control of anther development takes place via hormonal pathways, the wide action of which ensures reproductive success. In our previous research concerning flower and early pod development in yellow lupine, we showed that the lowest transcript level of LlDELLA1, a main repressor of gibberellin (GA) signalling, occurs approximately at the time of anther opening; therefore, the main purpose of this study was to precisely investigate the gibberellic acid (GA3)-dependent regulation of the anther dehiscence in this species. Results In this paper, we showed the specific changes in the yellow lupine anther structure during dehiscence, including secondary thickening in the endothecium by lignocellulosic deposition, enzymatic cell wall breakdown at the septum/stomium and cell degeneration via programmed cell death (PCD), and identified several genes widely associated with this process. The expression profile of genes varied over time, with the most intense mRNA accumulation in the phases prior to or at the time of anther opening. The transcriptional activity also revealed that these genes are highly coexpressed and regulated in a GA-dependent manner. The cellular and tissue localization of GA3 showed that these molecules are present before anther opening, mainly in septum cells, near the vascular bundle and in the endothecium, and that they are subsequently undetectable. GA3 localization strongly correlates with the transcriptional activity of genes related to GA biosynthesis and deactivation. The results also suggest that GA3 controls LlGAMYB expression via an LlMIR159-dependent pathway. Conclusions The presented results show a clear contribution of GA3 in the control of the extensive anther dehiscence process in yellow lupine. Understanding the processes underlying pollen release at the hormonal and molecular levels is a significant aspect of controlling fertility in this economically important legume crop species and is of increasing interest to breeders.

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Patterns of Expansion and Expression Divergence of the Polygalacturonase Gene Family in Brassica oleracea

Cited by 11 publications

References 52 publications

Genome-Wide identification and expression analysis of the polygalacturonase gene family in sweetpotato

Genome-Wide identification and expression analysis of the polygalacturonase gene family in sweetpotato

Anther Dehiscence Is Regulated By Gibberellic Acid In Yellow Lupine (Lupinus Luteus L.)

Anther dehiscence is regulated by gibberellic acid in yellow lupine (Lupinus luteus L.)

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