Patterns of gene action in plant development revealed by enhancer trap and gene trap transposable elements.

Sundaresan, Venkatesan; Springer, Patricia S.; Volpe, Thomas A.; Haward, Samuel; Jones, Jonathan D. G.; Dean, Caroline; Mā, Hong; Martienssen, Robert A.

doi:10.1101/gad.9.14.1797

Cited by 686 publications

(678 citation statements)

References 58 publications

(44 reference statements)

Supporting

Mentioning

665

Contrasting

Unclassified

Order By: Relevance

“…Vroemen (Wageningen University, The Netherlands). Construction of the gene trap and enhancer trap lines (Vroemen et al, 1998) was based on transposable elements of the Ac/Ds system carrying the GUS reporter gene (Sundaresan et al, 1995). For the selection procedure, seeds were surface sterilized with 2% (w/v) sodium hypochlorite in 50% (v/v) ethanol and sown on 0.6% (w/v) agar plates containing 0.46% (w/v) Murashige and Skoog (MS) salts (Duchefa BV, Haarlem, the Netherlands), 1.0% (w/v) sucrose and 50 mg l )1 kanamycin to select for plants with the gene/enhancer trap construct among the heterozygous lines.…”

Section: Gene Trap and Enhancer Trap Screeningmentioning

confidence: 99%

“…The gene trap lines were constructed to detect expression of a chromosomal gene with a transposon insertion within the transcribed region. For this purpose, the Ds element contained a promoterless GUS gene, whose expression relies on the transcription of the chromosomal gene in which it is inserted (Sundaresan et al, 1995;Vroemen et al, 1998). The enhancer trap lines contained a Ds element with the GUS gene fused to a minimal )1 to )46 bp CaMV 35S promoter, which is not active in the absence of enhancer sequences.…”

Section: Screening Transposants For P Fluorescens Wcs417r-induced Gumentioning

confidence: 99%

“…The enhancer trap lines contained a Ds element with the GUS gene fused to a minimal )1 to )46 bp CaMV 35S promoter, which is not active in the absence of enhancer sequences. In these lines, expression of the GUS reporter gene is dependent on insertion near chromosomal enhancer sequences (Sundaresan et al, 1995;Vroemen et al, 1998). Thus, when the Ds element of an enhancer trap line is inserted in the proximity of a chromosomal gene, within or outside the coding sequence, GUS gene expression can be activated.…”

Section: Screening Transposants For P Fluorescens Wcs417r-induced Gumentioning

confidence: 99%

“…In a complementary approach to identify novel defense-related genes that are potentially involved in rhizobacteria-mediated ISR, we screened a collection of gene trap and enhancer trap lines with transposable elements of the Ac/Ds system carrying the b-glucuronidase (GUS) reporter gene (Sundaresan et al, 1995;Vroemen et al, 1998), for genes that are specifically expressed in response to colonization of the roots by ISR-inducing WCS417r bacteria. Here, we identified a thaumatin-like gene (AtTLP1) that is specifically expressed in the root vascular bundle after colonization by fluorescent Pseudomonas spp., and after treatment with the ET precursor 1-aminocyclopropane-1-carboxylate (ACC).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Colonization of the Arabidopsis rhizosphere by fluorescent Pseudomonas spp. activates a root-specific, ethylene-responsive PR-5 gene in the vascular bundle

et al. 2005

View full text Add to dashboard Cite

Plants of which the roots are colonized by selected strains of non-pathogenic, fluorescent Pseudomonas spp. develop an enhanced defensive capacity against a broad spectrum of foliar pathogens. In Arabidopsis thaliana, this rhizobacteria-induced systemic resistance (ISR) functions independently of salicylic acid but requires responsiveness to jasmonic acid and ethylene. In contrast to pathogen-induced systemic acquired resistance (SAR), ISR is not associated with systemic changes in the expression of genes encoding pathogenesis-related (PR) proteins. To identify genes that are specifically expressed in response to colonization of the roots by ISRinducing Pseudomonas fluorescens WCS417r bacteria, we screened a collection of Arabidopsis enhancer trap and gene trap lines containing a transposable element of the Ac/Ds system and the GUS reporter gene. We identified an enhancer trap line (WET121) that specifically showed GUS activity in the root vascular bundle upon colonization of the roots by WCS417r. Fluorescent Pseudomonas spp. strains P. fluorescens WCS374r and P. putida WCS358r triggered a similar expression pattern, whereas ISR-non-inducing Escherichia coli bacteria did not. Exogenous application of the ethylene precursor 1-aminocyclopropane-1-carboxylate (ACC) mimicked the rhizobacteria-induced GUS expression pattern in the root vascular bundle, whereas methyl jasmonic acid and salicylic acid did not, indicating that the Ds element in WET121 is inserted in the vicinity of an ethylene-responsive gene. Analysis of the expression of the genes in the close vicinity of the Ds element revealed AtTLP1 as the gene responsible for the in cis activation of the GUS reporter gene in the root vascular bundle. AtTLP1 encodes a thaumatin-like protein that belongs to the PR-5 family of PR proteins, some of which possess antimicrobial properties. AtTLP1 knockout mutant plants showed normal levels of WCS417r-mediated ISR against the bacterial leaf pathogen Pseudomonas syringae pv. tomato DC3000, suggesting that expression of AtTLP1 in the roots is not required for systemic expression of ISR in the leaves. Together, these results indicate that induction of AtTLP1 is a local response of Arabidopsis roots to colonization by non-pathogenic fluorescent Pseudomonas spp. and is unlikely to play a role in systemic resistance.

show abstract

Section: Gene Trap and Enhancer Trap Screeningmentioning

confidence: 99%

Section: Screening Transposants For P Fluorescens Wcs417r-induced Gumentioning

confidence: 99%

Section: Screening Transposants For P Fluorescens Wcs417r-induced Gumentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Colonization of the Arabidopsis rhizosphere by fluorescent Pseudomonas spp. activates a root-specific, ethylene-responsive PR-5 gene in the vascular bundle

et al. 2005

View full text Add to dashboard Cite

show abstract

“…GUS stainings were carried out according to Sundaresan et al [11]. The sample was placed under vacuum for 5 min and incubated at 37°C overnight.…”

Section: Gus Reporter Assaysmentioning

confidence: 99%

Two differentially regulated Arabidopsis genes define a new branch of the DFR superfamily

et al. 2001

View full text Add to dashboard Cite

Two tandem genes were identified on Arabidopsis chromosome II (AtCRL1 and AtCRL2) encoding proteins with homology to members of the dihydroflavonol-4-reductase (DFR) superfamily. The encoded CRL1 and CRL2 proteins share 87% mutual amino acid sequence identity whereas their promoter regions are highly divergent, suggesting differential regulation of the CRL genes. Phylogenetic analysis placed CRL1 and CRL2 in a separate branch of the DFR superfamily. Northern blotting showed strong AtCRL1 induction by abscisic acid (ABA), drought, and heat shock, and high expression level in seeds, thus resembling the expression pattern of late embryogenic abundant ABA-responsive genes. Differential expression of the two genes during plant development was confirmed in plants expressing transcriptional fusions between the two promoters and the Escherichia coli b-glucuronidase reporter gene. This showed that, whereas high expression of AtCRL1 in mature seeds declines during subsequent vegetative growth, transcriptional activity from the AtCRL2 promoter increases during vegetative growth. Expression of both genes is restricted to vascular tissue. Based upon their homology to proteins involved in lignin synthesis, we propose that AtCRL2 is involved in generating conducting tissue late in development, while AtCRL1 is involved in vascular tissue differentiation and/or synthesis in the germinating embryos.

show abstract

Clearing a path through the jungle: progress in Arabidopsis genomics

Bevan

Bancroft

Mewes³

et al. 1999

Bioessays

View full text Add to dashboard Cite

Progress in sequencing the genome of the model plant Arabidopsis is reviewed. The resulting analysis of the sequence indicates an information‐rich genome that is being tackled by a variety of high‐throughput approaches aimed at understanding the functions of plant genes. The information derived from these systematic studies is providing important new knowledge of biological processes found uniquely in plants for comparison with that obtained in other multicellular organisms. BioEssays 1999;21:110–120. © 1999 John Wiley & Sons, Inc.

show abstract

Patterns of gene action in plant development revealed by enhancer trap and gene trap transposable elements.

Abstract: The crucifer Arabidopsis thaliana has been used widely as a model organism for the study of plant development. We describe here the development of an efficient insertional mutagenesis system in Arabidopsis

Cited by 686 publications

References 58 publications

Colonization of the Arabidopsis rhizosphere by fluorescent Pseudomonas spp. activates a root-specific, ethylene-responsive PR-5 gene in the vascular bundle

Colonization of the Arabidopsis rhizosphere by fluorescent Pseudomonas spp. activates a root-specific, ethylene-responsive PR-5 gene in the vascular bundle

Two differentially regulated Arabidopsis genes define a new branch of the DFR superfamily

Clearing a path through the jungle: progress in Arabidopsis genomics

Contact Info

Product

Resources

About