Patterns of Gene Expression in the Bovine Corpus Luteum Following Repeated Intrauterine Infusions of Low Doses of Prostaglandin F2alpha1

Atlı, Mehmet Osman; Bender, R.W.; Mehta, Vatsal; Bastos, M. R.; Luo, Wenxiang; Vezina, Chad M.; Wiltbank, Milo C.

doi:10.1095/biolreprod.111.094870

Cited by 85 publications

(95 citation statements)

References 73 publications

(110 reference statements)

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“…Both pathways stimulate the secretion of pro-inflammatory cytokines. Similarly, proinflammatory gene expression increases during regression of the CL (Atli et al, 2012;Shirasuna et al, 2012). Therefore, an increase in both TLR2 and TLR4 mRNA and protein levels in rCL in the present study may indicate roles of TLR signaling in the proinflammatory cytokine-mediated regressing mechanism in bovine CL.…”

Section: Discussionsupporting

confidence: 56%

“…The use of GAPDH as a house-keeping gene has been previously validated (Atli et al, 2012). Furthermore, we also did validate the consistency of steady-state GAPDH concentrations in the different stages of CLs.…”

Section: Discussionmentioning

confidence: 84%

“…To clarify RT specificity, analyses were also performed with all components of the RT kit without including RNA (RT negative). Oligonucleotide primers for StAR, TLR2, TLR4, and GAPDH were designed using Primer3 from the sequences in the NCBI gene database or from published primer sequences (Atli et al, 2012). The primer pair sequences and product sizes are shown in Table 1.…”

Section: Rna Isolation Rt Reaction and Quantitative Pcrmentioning

confidence: 99%

“…The CL is composed of multiple cell types, including steroidogenic (large luteal cells and small luteal cells) and non-steroidogenic cells (endothelial cells, pericytes, fibrocytes and immune cells; Wiltbank 1994) and this mixture of cells altogether contribute to the formation and demise of the CL. For example, despite the fact that the PGF 2α receptor was only detected in large luteal cells where the first luteolytic signal was expected to start (Mamluk et al, 1998), our previous studies have shown that luteolytic induction occurs in endothelial cells earlier than in luteal cells (Atli et al, 2011;Atli et al, 2012). Although the role of immune cells in the CL was previously thought to be in phagocytosis following luteolysis (reviewed by Pate and Landis Keyes, 2001), the immune cell population and chemokines in the CL are affected during functional luteolysis in the early response to PGF 2α (Shirasuna et al, 2012).…”

Section: Introductionmentioning

confidence: 96%

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Toll-like receptor 2 and 4 expression in the bovine corpus luteum during the different stages of the estrous cycle

Atlı¹,

Köse²,

Kaya³

et al. 2017

Anim Reprod

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The aim of this study was to elucidate the presence of components of the innate immune system in the bovine corpus luteum (CL) by detecting the expression and cell-specific localization of TLR2 and TLR4 during different stages of the estrous cycle in a control study design. Bovine CL samples were collected from a local slaughterhouse and assigned to three groups as follows: developing CL (dCL; n = 6, approx. days 3-6), mature CL (mCL; n = 5, approx. days 8-12), and regressing CL (rCL; n = 5, approx. days 17-19). An upregulation of TLR2 mRNA was detected only in rCL (P < 0.05). Localization of the TLR2 protein was particularly apparent in luteal cells and a prominent immunofluorescent signal corresponding to TLR2 was detected only in rCL. TLR4 mRNA were higher in mCL and rCL compared to dCL (P < 0.05). The presence of the TLR4 protein in bovine CL was clearly detected in the luteal cells of both mCL and rCL. The results of this study suggest a role for TLRs in the development, maintenance, and regression of bovine CL. TLR signaling mediated pathway in luteal cells may involve in the regression of CL via regulation of TLR2 and TLR4.

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Section: Discussionsupporting

confidence: 56%

Section: Discussionmentioning

confidence: 84%

Section: Rna Isolation Rt Reaction and Quantitative Pcrmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

See 2 more Smart Citations

Toll-like receptor 2 and 4 expression in the bovine corpus luteum during the different stages of the estrous cycle

Atlı¹,

Köse²,

Kaya³

et al. 2017

Anim Reprod

Self Cite

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“…This method allowed repeated biopsy sampling from a single CL without impairing its subsequent function (Tsai et al 2001, Atli et al 2012.…”

Section: Biopsiesmentioning

confidence: 99%

LPS-mediated effects and spatio-temporal expression of TLR2 and TLR4 in the bovine corpus luteum

Lüttgenau¹,

Herzog²,

Strüve³

et al. 2016

Reproduction

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When given intravenously (iv), lipopolysaccharide (LPS) transiently suppresses the structure and function of the bovine corpus luteum (CL). This is associated with increased release of prostaglandin (PG) F 2a metabolite. The underlying regulatory mechanisms of this process remain, however, obscure. Therefore, the aims of this study were: i) to investigate the expression of the LPS receptor toll-like receptor 4 (TLR4) and 2 (TLR2) in the bovine CL during early, mid-and late luteal phases; and ii) to further dissect the mechanisms of LPS-mediated suppression of luteal function. As revealed by semi-quantitative qPCR and immunohistochemistry, both receptors were detectable throughout the luteal lifespan. Their mRNA levels increased from the early toward the mid-luteal phase; no further changes were observed thereafter. The TLR4 protein seemed more highly represented than TLR2. The cellular localization of TLRs was in blood vessels; weaker signals were observed in luteal cells. Additionally, cows were treated either with LPS (iv, 0.5 mg/kg BW) or with saline on Day 10 after ovulation. Samples were collected 1200 h after treatment and on Day 10 of the respective subsequent (untreated) cycle. The mRNA expression of several possible regulatory factors was investigated, revealing the suppression of PGF 2a receptor (PTGFR), STAR protein and 3b-hydroxysteroid dehydrogenase, compared with controls and subsequent cycles. The expression of TLR2 and TLR4, interleukin 1a (IL1A) and 1b (IL1B) and of PGF 2a and PGE 2 synthases (HSD20A and mPTGES respectively) was increased. The results demonstrate the presence of TLR2 and TLR4 in the bovine CL, and implicate their possible involvement in the deleterious effects of LPS on its function.

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Changes in the expression of prostaglandin family members in bovine corpus luteum during the estrous cycle and pregnancy

Berisha

Schams

Rodler

et al. 2018

Molecular Reproduction Devel

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The aim of this study was to characterize certain prostaglandin family members in the bovine corpus luteum (CL) during the estrous cycle and pregnancy. The CL tissue was assigned to the stages 1-2, 3-4, 5-7, 8-12, 13-16 and >18 days (after regression) of the estrous cycle and 1-2, 3-4, 6-7, and >8 months of pregnancy. In these samples, we investigated prostaglandin F2alpha (PTGF), prostaglandin E2 (PTGE), their receptors (PTGFR, PTGER2, and PTGER4), cyclooxygenase 2 (COX-2), PTGF synthase (PTGFS), and PTGE synthase (PTGES). The expression of messenger RNA (mRNA) was measured by reverse transcription quantitative polymerase chain reaction, hormones by enzyme immunoassay, and localization by immunohistochemistry. The mRNA expression of COX-2, PTGFS, and PTGES in CL during the early-luteal phase was high followed by a continuous and significant downregulation afterward, as well as during all phases of pregnancy. The concentration of PTGF in CL tissue was high during the early-luteal phase, decreased significantly in the mid-luteal phase, and increased again afterward. In contrast, the concentration of PTGE increased significantly during the late-luteal phase followed by a decrease during regression. The PTGE level increased again during late pregnancy. Immunohistochemically, the large granulose-luteal cells show strong staining for COX-2 and PTGES during the early-luteal stage followed by lower activity afterward. During pregnancy, most of the luteal cells were only weakly positive or negative. In conclusion, our results indicate that the examined prostaglandin family members are involved in the local mechanisms that regulate luteal function, specifically during CL formation, function, and regression and during pregnancy in the cow.

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Patterns of Gene Expression in the Bovine Corpus Luteum Following Repeated Intrauterine Infusions of Low Doses of Prostaglandin F2alpha1

Cited by 85 publications

References 73 publications

Toll-like receptor 2 and 4 expression in the bovine corpus luteum during the different stages of the estrous cycle

Toll-like receptor 2 and 4 expression in the bovine corpus luteum during the different stages of the estrous cycle

LPS-mediated effects and spatio-temporal expression of TLR2 and TLR4 in the bovine corpus luteum

Changes in the expression of prostaglandin family members in bovine corpus luteum during the estrous cycle and pregnancy

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