1999
DOI: 10.1139/gen-42-5-919
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Patterns of puffing activity in the salivary gland polytene chromosomes of the medfly <i>Ceratitis capitata</i>, during larval and prepupal development

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Cited by 9 publications
(51 citation statements)
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“…The first puffing cycle takes place during the last 6 h of the larval stage and the first 3 h of the prepupal stage, that is, from the time the larva jumps off the food until 3 h after puparium formation, while the second puffing cycle takes place about 20 h later, at about the middle prepupal stage. The changes in the activities of most of the 128 puffs described in the above study (Gariou-Papalexiou et al 1999) were correlated with the ecdysone titer in the hemolymph, suggesting that they may be under ecdysone control. In this report, we present the in vitro mode of response for the most characteristic ecdysoneregulated medfly puffs in tissue cultures, with respect to ecdysone, the protein synthesis inhibitor cycloheximide, and ecdysone withdrawal and readdition.…”
Section: Introductionmentioning
confidence: 52%
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“…The first puffing cycle takes place during the last 6 h of the larval stage and the first 3 h of the prepupal stage, that is, from the time the larva jumps off the food until 3 h after puparium formation, while the second puffing cycle takes place about 20 h later, at about the middle prepupal stage. The changes in the activities of most of the 128 puffs described in the above study (Gariou-Papalexiou et al 1999) were correlated with the ecdysone titer in the hemolymph, suggesting that they may be under ecdysone control. In this report, we present the in vitro mode of response for the most characteristic ecdysoneregulated medfly puffs in tissue cultures, with respect to ecdysone, the protein synthesis inhibitor cycloheximide, and ecdysone withdrawal and readdition.…”
Section: Introductionmentioning
confidence: 52%
“…As previously described (Mintzas et al 1983), this strain has been maintained in our laboratory for more than 20 years under standard conditions (25 ± 1ºC, 40-50% relative humidity, and 12 h light : 12 h dark). Larvae were synchronized at both oviposition and larval hatching as described in Gariou-Papalexiou et al (1999). Grace's Insect Tissue Culture Medium (Serva) was prepared according to the manufacturer's instructions.…”
Section: Animals and Chemicalsmentioning
confidence: 99%
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