pCMV-Leu2/pUCA-Neo, a vector set for screening Schizosaccharomyces pombe transformants expressing heterologous proteins

Terazawa, Yumiko; Wakiyama, Motoaki; Yokoyama, Shigeyuki

doi:10.1016/j.ab.2011.03.034

Cited by 2 publications

(2 citation statements)

References 8 publications

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“…Expression of TGF-b in A549 and H358 cells was used for further analysis after 72 h transfection. Cells successfully TGF-b-trans-fected were selected using G418 (600 mg/L) screening for 4 times as described previously (24).…”

Section: Overexpression Of Tgf-bmentioning

confidence: 99%

Melittin suppresses growth and induces apoptosis of non-small-cell lung cancer cells via down-regulation of TGF-β-mediated ERK signal pathway

Wang

Han

2021

Braz J Med Biol Res

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The purpose of this study was to investigate the anti-cancer effect of melittin on growth, migration, invasion, and apoptosis of non-small-cell lung cancer (NSCLC) cells. This study also explored the potential anti-cancer mechanism of melittin in NSCLC cells. The results demonstrated that melittin suppressed growth, migration, and invasion, and induced apoptosis of NSCLC cells in vitro. Melittin increased pro-apoptotic caspase-3 and Apaf-1 gene expression. Melittin inhibited tumor growth factor (TGF)-b expression and phosphorylated ERK/total ERK (pERK/tERK) in NSCLC cells. However, TGF-b overexpression (pTGF-b) abolished melittin-decreased TGF-b expression and pERK/tERK in NSCLC cells. Treatment with melittin suppressed tumor growth and prolonged mouse survival during the 120-day observation in vivo. Treatment with melittin increased TUNEL-positive cells and decreased expression levels of TGF-b and ERK in tumor tissue compared to the control group. In conclusion, the findings of this study indicated that melittin inhibited growth, migration, and invasion, and induced apoptosis of NSCLC cells through down-regulation of TGF-b-mediated ERK signaling pathway, suggesting melittin may be a promising anti-cancer agent for NSCLC therapy.

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Section: Overexpression Of Tgf-bmentioning

confidence: 99%

Melittin suppresses growth and induces apoptosis of non-small-cell lung cancer cells via down-regulation of TGF-β-mediated ERK signal pathway

Wang

Han

2021

Braz J Med Biol Res

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“…Because of the intensive use of Sc. pombe in both fundamental and applied research, numerous papers describe the use of (integration-type) plasmids for molecular manipulation (reviewed by Siam et al ., 2004; more recent studies by Erler et al ., 2006; Chino et al ., 2010; Terazawa et al ., 2011; Verma & Singh, 2012). Interestingly, notwithstanding the vast phylogenetic distance separating the two species, transformation protocols from S. cerevisiae can usually be used in Sc.…”

Section: Genetic Modificationmentioning

confidence: 99%

Improving industrial yeast strains: exploiting natural and artificial diversity

Steensels¹,

Snoek²,

Meersman³

et al. 2014

FEMS Microbiol Rev

404

288

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Yeasts have been used for thousands of years to make fermented foods and beverages, such as beer, wine, sake, and bread. However, the choice for a particular yeast strain or species for a specific industrial application is often based on historical, rather than scientific grounds. Moreover, new biotechnological yeast applications, such as the production of second-generation biofuels, confront yeast with environments and challenges that differ from those encountered in traditional food fermentations. Together, this implies that there are interesting opportunities to isolate or generate yeast variants that perform better than the currently used strains. Here, we discuss the different strategies of strain selection and improvement available for both conventional and nonconventional yeasts. Exploiting the existing natural diversity and using techniques such as mutagenesis, protoplast fusion, breeding, genome shuffling and directed evolution to generate artificial diversity, or the use of genetic modification strategies to alter traits in a more targeted way, have led to the selection of superior industrial yeasts. Furthermore, recent technological advances allowed the development of high-throughput techniques, such as ‘global transcription machinery engineering’ (gTME), to induce genetic variation, providing a new source of yeast genetic diversity.

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pCMV-Leu2/pUCA-Neo, a vector set for screening Schizosaccharomyces pombe transformants expressing heterologous proteins

Cited by 2 publications

References 8 publications

Melittin suppresses growth and induces apoptosis of non-small-cell lung cancer cells via down-regulation of TGF-β-mediated ERK signal pathway

Melittin suppresses growth and induces apoptosis of non-small-cell lung cancer cells via down-regulation of TGF-β-mediated ERK signal pathway

Improving industrial yeast strains: exploiting natural and artificial diversity

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