PCR Analysis for Genetic Distances of Two Charybdis Crab Populations

Abstract

Genomic DNA (gDNA) set apart from two populations of Korean Charybdis crab (Charybdis japonica) was augmented by PCR experiments. The five oligonucleotides primers (ONTprimers) were spent to yield the number of unique loci shared to each crab population (ULSECP) and number of loci shared by the two crab populations (LSTCP). 305 fragments (FRAGs) were identified in the Charybdis crab population A (CCPA), and 344 in the Charybdis crab population B (CCPB): 44 number of ULSECP (14.43%) in the CCPA and 110 (31.98%) in the CCPB. 44 number of LSTCP, with an average of 8.8 per primer, were detected in the two crab populations. The bandsharing (BS) value between entity's no. 01 and no. 10 was the lowest (0.371) between the two CCPs. The average bandsharing (ABS) values of individuals in the CCPA (0.575±0.014) were lesser than in those originated from the CCPB (0.705±0.011) (p < 0.05). The polar hierarchical dendrogram (PHD) achieved by the five ONT-primers denotes three genetic clusters (GCs): cluster I (

Genetic Distances of Binary Pen Shell Atrina pectinata Populations

“…Related discretely, the ABS grades of the entities PSTA were higher than those from PSTB. To discriminate, the ABS amounts of individuals in the Charybdis crab population A (CCPA) (0.575±0.014) were lesser than in those originated from the Charybdis crab population B (CCPB) (0.705±0.011) ( p <0.05) ( Yoon, 2022 ), and entities from Cyclina sinensis species (0.754) displayed higher BS amounts than did entities from Saxidomus purpuratus species (0.607) ( p <0.05) in the three venerid shellfish species ( Jeon & Yoon, 2015 ).…”

“…This modus operandi might help define the inquisitive pointers related to the lines, species, genera, and geographic populations identification in teleost ( Garcia et al, 2004 ; Araneda et al, 2005 ; Siti Azizah et al, 2005 ; Upadhyay et al, 2006 ), and crustaceans ( Esselman et al, 2000 ; Huang et al, 2000 ; McCormack et al, 2000 ; Dixon et al, 2004 ; Henry & Kenchington, 2004 ; Fernandez-Tajes et al, 2007 ; Mahmoud et al, 2010 ; Yoon, 2022 ). By way of this research, it is possible a little to contribute to expanding the farming of pen shells, maintenance of clam species, protection of the natural environment, and conservation of ecosystems.…”