PCR Assays Detect B-Lymphocyte Clonality in Formalin-Fixed, Paraffin-Embedded Specimens of Classical Hodgkin Lymphoma Without Microdissection

Abstract

Hodgkin lymphoma (HL) was shown to be a B cell malignancy using PCR-clonality studies of microdissected Reed-Sternberg cells. While methods for the detection of B cell clonality could aid in the diagnosis of HL, microdissection is not practical in most clinical settings. We assessed the standardized BIOMED-2 IGH and IGK PCR primers for the detection of clonality using 50 consecutively diagnosed formalin-fixed paraffin-embedded (FFPE) classic Hodgkin lymphoma specimens. Without microdissection, clonality was detected in 23/47 assessable cases. The IGK assay was significantly more sensitive than the IGH assay (18 vs. 10 positive results). These data, and two representative cases, demonstrate that PCR based B-cell clonality assays have utility when the histologic differential diagnosis of an FFPE specimen includes classic Hodgkin lymphoma.

Dynamic balance of multiple myeloma clonogenic side population cell percentages controlled by environmental conditions

“…PCR primer sequences for IGH variable gene framework region 3 analysis was designed as reported previously, and PCR was used to assess clonality using the BIOMED‐2 system . PCR master mixes were purchased from Invivoscribe Technologies (San Diego, CA), and PCR was performed as per the manufacturer's instructions using HotStart Taq DNA polymerase (Qiagen, Valencia, CA) …”

New developments in the pathology of malignant lymphoma: a review of the literature published from April 2010–July 2010

“…An earlier study published in this journal [70] was confirmed by Burack et al [71] who show that without micro dissection HL samples have often a detectable clonal B-cell population (23/47); using primers for the Kappadeleting element locus is more sensitive than using those for the framework regions.…”

Clonality testing of lymphoproliferative disorders in a large cohort of primary and consultant biopsies

“…The sensitivity for detecting clonality in classical Hodgkin lymphoma (cHL) was the lowest (45%) in our study, probably due to the scarcity of tumor cells residing in an abundant admixture of non-malignant cells of different types that produces a polyclonal background signal and due to a high rate of somatic mutations in V regions 1,[44][45][46][47] . 39,45,50 ). In the group of T-cell lymphomas, the most successful detection of the PCR amplification was achieved for ALK + anaplastic large cell lymphoma (ALCL, 84.6%) and then for unspecified peripheral T-cell lymphoma (PTCLU, 83.6%).…”