2009
DOI: 10.1373/clinchem.2008.113035
|View full text |Cite
|
Sign up to set email alerts
|

PCR-Based Methods for the Enrichment of Minority Alleles and Mutations

Abstract: BACKGROUND:The ability to identify low-level somatic DNA mutations and minority alleles within an excess wild-type sample is becoming essential for characterizing early and posttreatment tumor status in cancer patients. Over the past 2 decades, much research has focused on improving the selectivity of PCR-based technologies for enhancing the detection of minority (mutant) alleles in clinical samples. Routine application in clinical and diagnostic settings requires that these techniques be accurate and cost-eff… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3

Citation Types

3
140
0
1

Year Published

2009
2009
2024
2024

Publication Types

Select...
7

Relationship

1
6

Authors

Journals

citations
Cited by 159 publications
(144 citation statements)
references
References 37 publications
3
140
0
1
Order By: Relevance
“…The aforementioned methods usually require nanogram amounts of mutated DNA. Altogether, these data support the need of an additional enrichment step to reach high sensitivity as was already postulated (32).…”
Section: Discussionsupporting
confidence: 84%
“…The aforementioned methods usually require nanogram amounts of mutated DNA. Altogether, these data support the need of an additional enrichment step to reach high sensitivity as was already postulated (32).…”
Section: Discussionsupporting
confidence: 84%
“…Furthermore, highly sensitive methods are required for the detection and identification of somatic mutations in early tumorigenesis and the emergence of resistance mutations (Milbury, et al, 2009b). Traditional Sanger sequencing has been considered the gold standard for detecting and identifying genetic alterations.…”
mentioning
confidence: 99%
“…Such subtle differences, however, may not provide clear discrimination between wild-type and mutant DNA, which is responsible for the low enrichment rate and difficulty in adequate thermal control. 3 The PNA/LNA-mediated PCR clamping technique is, in a sense, a method based on thermal differentiation. PNA is a DNA mimic in which the phosphoribose backbone is replaced by a peptide-like repeat of 2-aminoethyl-glycine units.…”
Section: Discussionmentioning
confidence: 99%
“…In principle, this does not aberrantly alter the wild-type sequences and leads to much lower false-positive rates. 3 The PNA/LNA-mediated clamping technique has been used for over a decade and is an attractive method that has proven to be potentially applicable to many situations, including the examination of various cancer mutations, 8,19 -23 identification of viral or bacterial variants, 24,25 genotyping for single-base substitutions, 26,27 detection of low-level somatic mosaicism 28 or mitochondrial heteroplasmy, 29,30 and prenatal diagnosis via maternal plasma, 31 among many others. However, the high cost and limited availability have restricted its usage.…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation