PCR-restriction endonuclease analysis for identification and strain typing ofMycobacterium aviumsubsp.paratuberculosisandMycobacterium aviumsubsp.aviumbased on polymorphisms in IS1311

Marsh, Ian; Whittington, Richard J.; Cousins, DV

doi:10.1006/mcpr.1999.0227

Cited by 147 publications

(109 citation statements)

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“…To distinguish subtypes of Map, deer isolates were assayed by IS1311 PCR-REA at the Biochemistry and Microbiology Department, Universidad Austral de Chile according to the protocol described by (Marsh et al 1999). The previously described primers M56 (59-GCGTGAGGCTCT-GTGGTGAA-39) and M94 (59-CAGCGATCGTCGA-CAGTGTG-39) were used to amplify a region of the IS1311 insertion sequence (Whittington et al 1998, Marsh et al 1999.…”

Section: Typingmentioning

confidence: 99%

“…The previously described primers M56 (59-GCGTGAGGCTCT-GTGGTGAA-39) and M94 (59-CAGCGATCGTCGA-CAGTGTG-39) were used to amplify a region of the IS1311 insertion sequence (Whittington et al 1998, Marsh et al 1999. Briefly, a reaction volume of 50 ml containing 5 ml of the DNA sample, 4 ml of each primer (10 pmol/ ml), 22.6 ml of water, 4 ml dNTPs (10mM), 5 ml MgCl2 (25 mM), 5 ml PCR buffer II (103), and 0.4 ml Taq Gold polymerase (5 U/ml) was used.…”

Section: Typingmentioning

confidence: 99%

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Paratuberculosis or avian tuberculosis in red deer with chronic diarrhea?

et al. 2014

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SUMMARYMycobacterium avium subsp. paratuberculosis (Map) is the etiological agent of paratuberculosis. In Chile, information about Map isolation from both domestic ruminant and wildlife species has been accumulating, but it has extended to other species. In Chile, deer farming activity has been focused in hunting and meat production. No paratuberculosis surveillance has been reported for these source herds. In the present study, chronic diarrhea and poor body condition in some animals belonging to a deer farm was informed. Four adult affected hinds were euthanized under suspicion of paratuberculosis. In all animals macroscopic and microscopic lesions consistent with paratuberculosis were observed. Map-positive culture results from faecal and tissue samples, complemented with positive-PCR-results from fixed tissues are informed. Evidence has been found on cattle and deer populations being connected since molecular characterization of the Map isolated from deer lack of variation between the cattle control strain. The latter could indicate that both species share the same bacteria, suggesting there is interspecies transmission. Complementary diagnostic methods were accurate to diagnose paratuberculosis and to differentiate the clinical deer case from other mycobacterial infection. The epidemiological findings suggest that the infection in the deer farm could be transmitted from a cattle herd, making this the first reported case of paratuberculosis in deer farm in Chile.Key words: deer, histopathology, paratuberculosis, PCR. RESUMENMycobacterium avium subsp. paratuberculosis (Map) es el agente etiológico de la patuberculosis. En Chile, antecedentes del aislamiento de Map tanto en rumiantes domésticos como en especies de vida silvestre se han ido acumulando a través del tiempo, sin embargo, esto tiene que estar afectando también a otras especies. En Chile, la crianza de ciervos se ha focalizado en dos objetivos: cotos de caza y producción de carnes exóticas, no existiendo un programa de vigilancia ni siendo reportada esta enfermedad en rebaños de este tipo. Este trabajo reporta la presencia de diarrea crónica y pobre condición corporal en algunos animales pertenecientes a una granja de ciervos donde se sospechó la presencia de paratuberculosis. Cuatro ciervos adultos fueron eutanaziados bajo esta sospecha. Todos los animales presentaron lesiones macro y microscópicas compatibles con paratuberculosis. Resultados positivos al cultivo bacteriológico a partir de heces y muestras de tejido, además de PCR a partir de tejido fijado evidencian la infección. De acuerdo a los resultados de caracterización molecular de las cepas aisladas de los ciervos que no muestran variación entre cepas control de bovino, se evidencia que ambas poblaciones estarían conectadas. Complementariamente, los métodos diagnósticos utilizados fueron muy precisos para diagnosticar paratuberculosis y para diferenciar casos clínicos en ciervos de otras micobacteriosis. Las características epidemiológicas descritas en este estudio sugieren que la infecció...

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Section: Typingmentioning

confidence: 99%

Section: Typingmentioning

confidence: 99%

Paratuberculosis or avian tuberculosis in red deer with chronic diarrhea?

et al. 2014

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“…A third Intermediate type (Type III) has been described, although it is considered to be closely related to Type I (Mö bius et al, 2009). Some of the techniques that have been described for distinguishing between these major strain types include restriction fragment length polymorphism (RFLP) analysis of IS900, PCR-restriction endonuclease analysis of IS1311, PCR of a discriminatory region, and sequencing or high resolution melt analysis of Map1506 (Marsh et al, 1999;Whittington et al, 2000;Collins et al, 2002;Griffiths et al, 2008;Castellanos et al, 2009). Domestic sheep and cattle are most commonly infected with their named strain type respectively, suggesting that there may be a certain degree of host specificity (Whittington et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

Occurrence, Diagnosis, and Strain Typing of Mycobacterium Avium Subspecies Paratuberculosis Infection in Rocky Mountain Bighorn Sheep (Ovis Canadensis Canadensis) in Southwestern Alberta

Forde

Kutz

Buck

et al. 2012

Journal of Wildlife Diseases

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ABSTRACT:The role that wildlife may play in the transmission of Mycobacterium avium subspecies paratuberculosis (Map), the causative agent of Johne's disease (JD), and the potential consequences of infection in these populations are being given increasing consideration. A yearling male Rocky Mountain bighorn sheep (Ovis canadensis canadensis) from southwestern Alberta, Canada, was found infected with Map in August 2009. Clinical signs of emaciation and diarrhea and histologic findings of diffuse granulomatous enteritis of the distal ileum, lymphadenitis of the mesenteric lymph nodes, and lymphangitis of the ileum were similar to previously described cases of JD in bighorn sheep. Infection with Map was confirmed by bacterial isolation through fecal culture, acid-fast staining, and polymerase chain reaction (PCR) of IS900. The Map1506 gene was sequenced, and the isolate was identified as a Cattle (Type II) strain. In a follow-up herd-level survey, three of 44 fecal samples (7%) from individual bighorn sheep from the same herd as the index case were PCRpositive and identified as Type II Map strains. Twenty-five samples from a distant bighorn population were negative. Additional strain typing of the isolates from the index case and the positive fecal samples was done by sequencing three discriminatory short sequence repeat (SSR) regions. All four SSR profiles differed from one another, suggesting multiple introductions or a long-existing circulation of Map within this bighorn population. Detailed molecular analyses are essential for understanding and managing diseases at the wildlife-livestock interface.

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“…A cultura foi positiva em 2,0% das 300 amostras cultivadas. Os resultados da técnica PCR-REA sobre as estirpes isoladas, que foram identificadas como pertencentes ao tipo S (ovino), estão de acordo com os observados por outros autores (Whittington et al 1998, Marsh et al 1999, Sevilla et al 2005. Quanto à técnica de diagnóstico utilizada, os fracos resultados observados quanto às taxas de isolamento em cultura fecal, são semelhantes aos referenciados em idên-ticos estudos em ovinos (Juste et al 1988, Carrigan & Seaman 1990, Collins et al 1990, 1993b, Shulaw et al 1993, mas inferiores a outros documentados (Adúriz et al 1995, Garrido 2002.…”

Section: Discussionunclassified

Comparação de duas técnicas de isolamento do Mycobacterium avium subsp. paratuberculosis em amostras de fezes de ovinos com suspeita clínica de paratuberculose

et al. 2009

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A paratuberculose é uma enterite crônica granulomatosa causada por Mycobacterium avium subsp. paratuberculosis que afeta principalmente os ruminantes. A cultura de bactérias a partir de amostras de fezes e tecidos constitui um dos métodos mais eficazes de diagnóstico, sendo ainda o único método disponível para obtenção de isolamentos e estirpes de micobactérias. Contudo, este método apresenta baixa sensibilidade e requer meses de incubação antes do crescimento de colônias. Neste estudo, utilizou-se a cultura fecal como método de diagnóstico em ovinos de diferentes raças portuguesas, com sinais compatíveis com a doença. Fez-se ainda a comparação entre os meios de cultura Löwenstein Jensen® com micobactina® J e o de Middlebrook® 7H11 com OADC®, utilizados no isolamento da bactéria. As percentagens de isolamento em cada um os meios foram de 2,0% (6/300) para Löwenstein Jensen® com micobactina J e 1,0% (3/300) para Middlebrook® 7H11/OADC. As três amostras positivas no meio de Middlebrook® 7H11/OADC também foram positivas no meio de Löwenstein Jensen® com micobactina J e nenhuma foi somente positiva no meio de Middlebrook® 7H11/OADC. Os resultados deste estudo sugerem que o meio de Löwenstein-Jensen® com micobactina® J é mais efetivo para a obtenção de estirpes ovinas em Portugal.

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PCR-restriction endonuclease analysis for identification and strain typing ofMycobacterium aviumsubsp.paratuberculosisandMycobacterium aviumsubsp.aviumbased on polymorphisms in IS1311

Cited by 147 publications

References 0 publications

Paratuberculosis or avian tuberculosis in red deer with chronic diarrhea?

Paratuberculosis or avian tuberculosis in red deer with chronic diarrhea?

Occurrence, Diagnosis, and Strain Typing of Mycobacterium Avium Subspecies Paratuberculosis Infection in Rocky Mountain Bighorn Sheep (Ovis Canadensis Canadensis) in Southwestern Alberta

Comparação de duas técnicas de isolamento do Mycobacterium avium subsp. paratuberculosis em amostras de fezes de ovinos com suspeita clínica de paratuberculose

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