2021
DOI: 10.1007/s00203-021-02668-x
|View full text |Cite|
|
Sign up to set email alerts
|

PCR system for the correct differentiation of the main bacterial species of the Klebsiella pneumoniae complex

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
1

Citation Types

0
4
0

Year Published

2022
2022
2024
2024

Publication Types

Select...
3

Relationship

1
2

Authors

Journals

citations
Cited by 3 publications
(4 citation statements)
references
References 23 publications
0
4
0
Order By: Relevance
“…pneumoniae isolates that were identified by biochemical tests or automated microbiological systems. Susbsequently, the proper differentiation of the main members of the KpSC was done by multiplex PCR 27 . Forty-seven isolates were eliminated from the study because they were duplicated or could not be assigned as K. pneumoniae, K. quasipneumonia e or K. variicola or belonged to a bacterial species outside of the KpSC.…”
Section: Methodsmentioning
confidence: 99%
“…pneumoniae isolates that were identified by biochemical tests or automated microbiological systems. Susbsequently, the proper differentiation of the main members of the KpSC was done by multiplex PCR 27 . Forty-seven isolates were eliminated from the study because they were duplicated or could not be assigned as K. pneumoniae, K. quasipneumonia e or K. variicola or belonged to a bacterial species outside of the KpSC.…”
Section: Methodsmentioning
confidence: 99%
“…Therefore, genetic identification by sequencing of the whole genome or the KVAR_0717 ( yggE ) gene is the most reliable method ( 4 ). Multiplex PCR targeting genes present only in each species of K. pneumoniae complex has also been proposed as a more widely available method ( 5 , 6 ). While matrix-assisted laser desorption ionization–time of flight (MALDI-TOF) mass spectrometry has been increasingly used for bacterial identification in clinical microbiology laboratories in recent years, previous studies reported that misidentification of the species belonging to K. pneumoniae complex frequently occurs by this method ( 4 , 7 , 8 ).…”
Section: Observationmentioning
confidence: 99%
“…Multiplex PCR for the unique genes of K. pneumoniae , K. variicola , and K. quasipneumoniae was performed as previously described ( 5 ). Fifty-five strains were identified as K. pneumoniae , 26 as K. variicola , and 2 as K. quasipneumoniae , and these results were used as the reference for later identification by MALDI-TOF.…”
Section: Observationmentioning
confidence: 99%
See 1 more Smart Citation