PCR to detect patulin producing moulds validated in foods

Luque, M. Isabel; Rodríguez, Alicia; Andrade, María J.; Gordillo, Rubén; Rodrı́guez, Mar; Córdoba, Juan J.

doi:10.1016/j.foodcont.2011.10.014

Cited by 1 publication

(1 citation statement)

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“…In parallel, Isabel Luque et al. (2012)) developed a sensitive and effective TaqMan qPCR multiplex method to estimate many mycotoxins as well as PAT‐producing fungi. For further development, Hosoya et al.…”

Section: Current Application Of Biotechnology In the Face Of The Foodmentioning

confidence: 99%

Recent trends in detecting, controlling, and detoxifying of patulin mycotoxin using biotechnology methods

Ngea

Yang

Castoria

et al. 2020

Comp Rev Food Sci Food Safe

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Patulin (PAT) is a mycotoxin that can contaminate many foods and especially fruits and fruit‐based products. Therefore, accurate and effective testing is necessary to enable producers to comply with regulations and promote food safety. Traditional approaches involving the use of chemical compounds or physical treatments in food have provided practical methods that have been used to date. However, growing concerns about environmental and health problems associated with these approaches call for new alternatives. In contrast, recent advances in biotechnology have revolutionized the understanding of living organisms and brought more effective biological tools. This review, therefore, focuses on the study of biotechnology approaches for the detection, control, and mitigation of PAT in food. Future aspects of biotechnology development to overcome the food safety problem posed by PAT were also examined. We find that biotechnology advances offer novel, more effective, and environmental friendly approaches for the control and elimination of PAT in food compared to traditional methods. Biosensors represent the future of PAT detection and use biological tools such as aptamer, enzyme, and antibody. PAT prevention strategies include microbial biocontrol, the use of antifungal biomolecules, and the use of microorganisms in combination with antifungal molecules. PAT detoxification aims at the breakdown and removal of PAT in food by using enzymes, microorganisms, and various adsorbent biopolymers. Finally, biotechnology advances will be dependent on the understanding of fundamental biology of living organisms regarding PAT synthesis and resistance mechanisms.

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Section: Current Application Of Biotechnology In the Face Of The Foodmentioning

confidence: 99%