PdbrlA, PdabaA and PdwetA control distinct stages of conidiogenesis in Penicillium digitatum

Wang, Mingshuang; Sun, Xuepeng; Zhu, Changqing; Xu, Qian; Ruan, Ruoxin; Yu, Dongliang; Li, Hongye

doi:10.1016/j.resmic.2014.12.003

Cited by 59 publications

(60 citation statements)

References 56 publications

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“…In P . digitatum , the lack of wetA also results in abnormal conidia, delayed germination, and reduced stress tolerance [15]. Moreover, P .…”

Section: Discussionmentioning

confidence: 99%

“…fumigatus , and P . digitatum [13,15] (Fig 2B and 2D). The condensation of the C2 layer along with the formation of the C3 and C4 layers are the final stage of conidial maturation, which contributes to the impermeability of the conidial wall [29].…”

Section: Discussionmentioning

confidence: 99%

“…WetA is essential for establishing the heat stress tolerance in Ascomycetes [13,15,17]. However, loss of wetA in different species results in variable degrees of tolerance to oxidative stress and osmotic stress.…”

Section: Discussionmentioning

confidence: 99%

“…bassiana [17] wetA deletion mutants showed WT level tolerance to H 2 O 2 and the P . digitatum [15] wetA deletion mutants even showed enhanced tolerance to H 2 O 2 . Loss of wetA causes reduced osmotic stress tolerance in A .…”

Section: Discussionmentioning

confidence: 99%

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WetA bridges cellular and chemical development in Aspergillus flavus

Mead

Kim

et al. 2017

PLoS ONE

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Bridging cellular reproduction and survival is essential for all life forms. Aspergillus fungi primarily reproduce by forming asexual spores called conidia, whose formation and maturation is governed by the central genetic regulatory circuit BrlA→AbaA→WetA. Here, we report that WetA is a multi-functional regulator that couples spore differentiation and survival, and governs proper chemical development in Aspergillus flavus. The deletion of wetA results in the formation of conidia with defective cell walls and no intra-cellular trehalose, leading to reduced stress tolerance, a rapid loss of viability, and disintegration of spores. WetA is also required for normal vegetative growth, hyphal branching, and production of aflatoxins. Targeted and genome-wide expression analyses reveal that WetA exerts feedback control of brlA and that 5,700 genes show altered mRNA levels in the mutant conidia. Functional category analyses of differentially expressed genes in ΔwetA RNA-seq data indicate that WetA contributes to spore integrity and maturity by properly regulating the metabolic pathways of trehalose, chitin, α-(1,3)-glucan, β-(1,3)-glucan, melanin, hydrophobins, and secondary metabolism more generally. Moreover, 160 genes predicted to encode transcription factors are differentially expressed by the absence of wetA, suggesting that WetA may play a global regulatory role in conidial development. Collectively, we present a comprehensive model for developmental control that bridges spore differentiation and survival in A. flavus.

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“…In P . digitatum , the lack of wetA also results in abnormal conidia, delayed germination, and reduced stress tolerance [15]. Moreover, P .…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

WetA bridges cellular and chemical development in Aspergillus flavus

Mead

Kim

et al. 2017

PLoS ONE

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“…Recent sequencing of the genome of different strains of P. digitatum (Marcet-Houben et al 2012;Sun et al 2013) and the development of genetic transformation protocols (Wang & Li 2008) have paved the way for molecular and genetic studies on this fungus (Gonz alez-Candelas et al 2010;Sun et al 2011) that include the generation of mutants to study gene function (Zhang et al 2013a;Zhang et al 2013b;Zhang et al 2013c;Gand ıa et al 2014;Wang et al 2014;Harries et al 2015;L opez-P erez et al 2015;Wang et al 2015;Zhu et al 2015). However, the generation of knockout mutants of P. digitatum is a difficult task due to the very low frequency of HR.…”

Section: Introductionmentioning

confidence: 99%

Disruption of ku70 involved in non-homologous end-joining facilitates homologous recombination but increases temperature sensitivity in the phytopathogenic fungus Penicillium digitatum

Gandía

Font

et al. 2016

Fungal Biology

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Involvement of LaeA in the regulation of conidia production and stress responses in Penicillium digitatum

Zhu¹,

Wang

et al. 2019

J Basic Microbiol

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Involvement of LaeA in various biological processes of filamentous fungi has been demonstrated. However, its role in Penicillium digitatum, the causal agent of citrus postharvest green mold, remains unclear. In this study, a ΔPdLaeA mutant was constructed using homologous recombination. The production of conidia by the ΔPdLaeA mutant was reduced by half compared with that of the wild‐type strain. The sensitivity of the ΔPdLaeA mutant increased under alkaline conditions. The virulence assay revealed that PdLaeA was dispensable for the virulence of P. digitatum. Comparative transcriptome analysis revealed that the function loss of PdLaeA resulted in the reduced expression of several secondary metabolite gene clusters. In addition, expression of several key regulators of conidiation (BrlA, FlbA, FlbC, FlbD, and FluG) was also downregulated in the ΔPdLaeA mutant. In summary, the present work demonstrated that PdLaeA was involved in the regulation of SM biosynthesis, as well as the development and environmental adaptation of P. digitatum.

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PdbrlA, PdabaA and PdwetA control distinct stages of conidiogenesis in Penicillium digitatum

Cited by 59 publications

References 56 publications

WetA bridges cellular and chemical development in Aspergillus flavus

WetA bridges cellular and chemical development in Aspergillus flavus

Disruption of ku70 involved in non-homologous end-joining facilitates homologous recombination but increases temperature sensitivity in the phytopathogenic fungus Penicillium digitatum

Involvement of LaeA in the regulation of conidia production and stress responses in Penicillium digitatum

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