PDCD5 regulates cell proliferation, cell cycle progression and apoptosi

Abstract

Abstract. Programmed cell death (PDCD)5 is cloned from human leukemia cell line TF-1. PDCD5 is one of the members of the programmed cell death protein family that is frequently involved in tumor growth and apoptosis. To investigate the molecular and cellular functions of PDCD5, the present study established a PDCD5 stably overexpressing A431 cell line and examined the role of PDCD5 in cell proliferation, cell cycle progression and apoptosis. The data demonstrated that overexpression of PDCD5 significantly inhibited cell proliferation, induced cell cycle arrest at G2/M phase and apoptosis in A431 cells. The expression profiles of certain key regulators of these cellular events were further investigated, including P53, B cell lymphoma (BCL)-2, BCL-2 associated X protein (BAX) and caspase (CASP)3. The data demonstrated that at the transcript and protein levels, P53, BAX and CASP3 were all upregulated in the PDCD5 stably overexpressing A431 cells whereas BCL-2 was downregulated, indicating that PDCD5 acts as an important upstream regulator of P53, BCL-2, BAX and CASP3. The data suggest that PDCD5 regulates cell proliferation, cell cycle progression and apoptosis in A431 cells. PDCD5 may be a novel tumor suppressor gene, and may be potentially used for cancer treatment in the future.

New Approach about the Signaling Crosstalk between IQGAPs/ NF- κB/IL-8 and PDCD5/p53/TRAIL Pathways that Modulate Malignant Transformation in Hepatocellular Carcinoma

“…Programmed cell death 5 (PDCD5), known also as TF-1 cell apoptosis-related gene-19 (TFAR19), was originally recognized in human leukemia TF-1 cells having serum withdrawal-induced apoptosis (Ha et al, 2021;Li et al, 2018). PDCD5 was declared downregulated in a lot of the human malignancies, including breast cancer (Hedenfalk et al, 2001) lung adenocarcinoma and hepatocellular carcinoma (Ma et al, 2019), as well as ovarian cancer (Gao et al, 2015).…”

<p>MicroRNA-766 Promotes The Proliferation, Migration And Invasion, And Inhibits The Apoptosis Of Cutaneous Squamous Cell Carcinoma Cells By Targeting PDCD5</p>

“…At present, twelve members of PDCD protein family are identified, including PDCD1-PDCD12. 16 It has reported that PDCD5 is lowly expressed in various tumors, such as lung cancer, 29 gastric cancer 30 and liver cancer. 31 In this study, we also confirmed that PDCD5 expression was downregulated in CSCC tissue and cells.…”

“…Programmed cell death 5 (PDCD5), one of the programmed cell death proteins, is abnormally expressed in many cancers and plays an important role in cell proliferation, apoptosis and metastasis. [14][15][16] Fan et al 17 have indicated that PDCD5 can inhibit cell growth and invasion, and promote cell apoptosis in hepatic cancer. However, whether the regulatory effect of miR-766 on CSCC is related to PDCD5 is unknown.…”

The Molecular Evolution and Functional Divergence of Lamprey Programmed Cell Death Genes

“…Numerous studies have demonstrated that H-PDCD5 promotes its cleavage and activation, leading to the occurrence of apoptosis (59, 60). H-PDCD5 causes cell apoptosis by regulating the expression of BCL-2 family members (61). For the TP53 gene, the presence of PDCD5 and drug treatment increases the activity of TP53, thereby increasing the interaction between PDCD5 and TP53 to induce cell apoptosis (62).…”