PDGF isoforms induce and maintain anagen phase of murine hair follicles

Abstract

SummaryBackground: It is known that platelet-derived growth factor (PDGF) receptors are expressed in hair follicle (HF) epithelium. Objectives:The aim of the present study was to clarify the effects of PDGF-AA and -BB on the cyclic growth of HFs.Methods: PDGF-AA or -BB was injected into the dorsal skin of C3H mice during the second telogen phase once daily for five consecutive days, or PDGF-AA or -BB dissolved in hyaluronic acid was injected only once. In order to confirm the effects of different PDGF isoforms, anti-PDGF-AA antibody or anti-PDGF-BB antibody was injected just after each injection of PDGF-AA or -BB. In addition, anti-PDGF antibodies were injected into the skin of C3H mice during the second anagen phase once daily for five days. We studied expression of signaling molecules in the skin where anagen phase had been induced by PDGF injection by real time-RT-PCR. Results: Both PDGF-AA and -BB injection experiments immediatelyinduced the anagen phase of the hair growth cycle at the injection sites.The induction of anagen was interfered by anti-PDGF antibody treatment.Real time-RT-PCR using extracted RNA from the PDGF injected sites of skin samples showed upregulated expression of HF differentiation-related key signaling molecules, Sonic hedgehog (Shh), Lef-1 and Wnt5a. Conclusions:These results indicate that both PDGF-AA and -BB are involved in the induction and maintenance of the anagen phase in the mouse hair cycle. Local application of PDGF-AA and -BB might therefore prove to be an effective treatment option for alopecia associated with early catagen induction and elongated telogen phase.2

Hair growth stimulated by conditioned medium of adipose-derived stem cells is enhanced by hypoxia: evidence of increased growth factor secretion

“…(30). In addition, PDGF and its receptors are important in follicular development, and PDGF isoforms induce and maintain the anagen phase in murine hair follicles (27). IGF-I also up-regulates hair follicle growth in various systems (26,29).…”

“…In a VEGF-transgenic mouse model, VEGF controlled hair growth and follicle size by the modulation of angiogenesis (30). PDGF isoforms induce and maintain the anagen phase in murine hair follicles (27). HGF and IGF also up-regulate hair follicle growth in various systems (9,20,26,29).…”

Nuclear Factor I-C Regulates TGF-β-dependent Hair Follicle Cycling*

“…Proinflammatory cytokines or growth factors released after plucking may be responsible for anagen phase induction. For instance, PDGF, a potent chemotactic agent for macrophages, is known to activate the growth of telogen follicles (49). We recently found that NFI-C is involved in the inflammatory phase of the skin healing process (9).…”

Role of platelet-derived growth factors in physiology and medicine

“…Postnatal PDGF-A-null mice show progressive loss of dermal mesenchyme and reduced hair development . Conversely, dermal injection of PDGF-AA or PDGF-BB in mice triggers resting hair follicles to enter the hair growth cycle (Tomita et al 2006). The role of PDGF-A in the developing skin appears to be to secure the renewal of PDGFR-␣-positive mesenchymal cells that are consumed as they are recruited to hair follicles to become dermal papillae and dermal sheaths ( Fig.…”