PdIrBP mesoporous nanospheres combined with superconductive carbon black for the electrochemical determination and collection of circulating tumor cells

Yang, Peng; Peng, Yuhang; Tang, Sitian; Shen, Huawei; Sheng, Shangchun; Wang, Yonghong; Wang, Teng; Cai, Juan; Feng, Wenli

doi:10.1007/s00604-020-4213-z

Cited by 23 publications

(12 citation statements)

References 40 publications

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“…CTCs. Development of nanotechnology has included the adaptation of numerous state-of-the-art nanoscale materials, such as metals, metal oxides, semiconductors, liposomes, graphene and graphene oxide, into nanoparticles, nanowires, nanofibers, nanopillars, and nanotubes (18,(35)(36)(37)(38)(39)(40). These nanomaterials have been successfully incorporated into platforms capable of detecting CTCs.…”

Section: Application Of Nanomaterials For Isolation and Detection Ofmentioning

confidence: 99%

Detection of circulating tumor cells: Advances and critical concerns (Review)

Zang

2021

Oncol Lett

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Metastasis is the main cause of cancer-related death and the major challenge in cancer treatment. Cancer cells in circulation are termed circulating tumor cells (CTCs). Primary tumor metastasis is likely due to CTCs released into the bloodstream. These CTCs extravasate and form fatal metastases in different organs. Analyses of CTCs are clarifying the biological understanding of metastatic cancers. These data are also helpful to monitor disease progression and to inform the development of personalized cancer treatment-based liquid biopsy. However, CTCs are a rare cell population with 1-10 CTCs per ml and are difficult to isolate from blood. Numerous approaches to detect CTCs have been developed based on the physical and biological properties of the cells. The present review summarizes the progress made in detecting CTCs. Contents 1. Introduction 2. Novel materials for enrichment and detection of CTCs 3. Novel technologies for isolation and detection of CTCs 4. Discussion

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Section: Application Of Nanomaterials For Isolation and Detection Ofmentioning

confidence: 99%

Detection of circulating tumor cells: Advances and critical concerns (Review)

Zang

2021

Oncol Lett

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“…Hence, electrochemical biosensors have been mostly hybridized with different materials, such as immunoassays, nanoparticles, composites, magnetic beads, nanowires, transistors, and nanosheets, as illustrated in Figure 3e. [159][160][161][162][163][164] To exemplify, an electrochemical assay system based on PdIrBPMNS (palladium-iridiumboron-phosphorus alloy-modified mesoporous nanospheres) and KB (Ketjen black)/AuNPs was designed for the isolation of MCF-7 cells. [159] Capture antibodies and signal antibodies chosen in this work were a cocktail of anti-vimentin antibodies and anti-EpCAM antibodies in order to improve the poor clinical relevance of the detection resulted from EMT.…”

Section: Electrochemical-based Releasementioning

confidence: 99%

Smart Material‐Integrated Systems for Isolation and Profiling of Rare Cancer Cells and Emboli

Sagdic

İnci

2021

Adv Eng Mater

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This review presents a broad aspect of smart material‐integrated systems for isolating and profiling rare circulating tumor cells (CTCs) and circulating tumor microemboli (CTM) to provide physiological, biological, and mechanistic insights into cancer research. In particular, CTCs/CTM have emanated as essential pieces of evidence that can reveal clonal evolution, tumor heterogeneity, and disease progression within the metastatic cascade. Morphologies, cellular compositions, and rarity of CTCs/CTM make them difficult to track and isolate for profiling distinct molecular characteristics in the case of metastatic potential. Accordingly, with the advanced‐characterization techniques, examining the aspects of the specific surface markers of CTCs/CTM, epithelial‐to‐mesenchymal (EMT), mesenchymal‐to‐epithelial (MET) transitions, and timing of tumor cell dissemination would assist us to understand cancer biology and metastatic characteristics. Existing clinical and research methods for the enrichment and isolation of these sporadic cells depend on mainly conventional methods with low‐yield and expensive features. Owing to their specialized functions and analytical performances, smart material‐based technologies hold an enormous impact not only on cell detection, but also on cell isolation for downstream analyses. Herein, the main reasons for cell isolation are discussed and the recent developments in CTCs/CTM approaches for identifying further methods and future perspectives are elaborated on.

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“…Presently, academic attention has shifted toward electrochemical biosensors that utilize noninvasive and highly sensitive nanomaterials ( Thévenot et al, 2001 ; Wang, 2005 a ; b ; 2006 ; Peng et al, 2020 ). For example, by combining aptamer and gold-magnetic core-shell nanoparticles, Khoshfetrat fabricated an aptamer-based electrochemical biosensor with a nitrogen-doped graphene-modified electrode.…”

Section: Introductionmentioning

confidence: 99%

Multifunctional Gold Nano-Cytosensor With Quick Capture, Electrochemical Detection, and Non-Invasive Release of Circulating Tumor Cells for Early Cancer Treatment

Zhang

You

Cheng

et al. 2021

Front. Bioeng. Biotechnol.

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Circulating tumor cells (CTCs) are metastatic tumor cells that shed into the blood from solid primary tumors, and their existence significantly increases the risk of metastasis and recurrence. The timely discovery and detection of CTCs are of considerable importance for the early diagnosis and treatment of metastasis. However, the low number of CTCs hinders their detection. In the present study, an ultrasensitive electrochemical cytosensor for specific capture, quantitative detection, and noninvasive release of EpCAM-positive tumor cells was developed. The biosensor was manufactured using gold nanoparticles (AuNPs) to modify the electrode. Three types of AuNPs with controllable sizes and conjugated with a targeting molecule of monoclonal anti-EpCAM antibody were used in this study. Electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV) of the cytosensors were performed to evaluate the cell capture efficiency and performance. The captured 4T1 cells by the AuNPs hindered electron transport efficiency, resulting in increased EIS responses. The cell capture response recorded using EIS or DPV indicated that the optimal AuNPs size should be 17 nm. The cell capture response changed linearly with the concentration range from 8.0 × 10 to 1 × 107 cells/mL, and the limit of detection was 50 cells/mL. After these measurements, glycine-HCl (Gly-HCl) was used as an antibody eluent to destroy the binding between antigen and antibody to release the captured tumor cells without compromising their viability for further clinical research. This protocol realizes rapid detection of CTCs with good stability, acceptable assay precision, significant fabrication reproducibility with a relative standard deviation of 2.09%, and good recovery of cells. Our results indicate that the proposed biosensor is promising for the early monitoring of CTCs and may help customize personalized treatment options.

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PdIrBP mesoporous nanospheres combined with superconductive carbon black for the electrochemical determination and collection of circulating tumor cells

Cited by 23 publications

References 40 publications

Detection of circulating tumor cells: Advances and critical concerns (Review)

Detection of circulating tumor cells: Advances and critical concerns (Review)

Smart Material‐Integrated Systems for Isolation and Profiling of Rare Cancer Cells and Emboli

Multifunctional Gold Nano-Cytosensor With Quick Capture, Electrochemical Detection, and Non-Invasive Release of Circulating Tumor Cells for Early Cancer Treatment

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