PDMS microchip coated with polydopamine/gold nanoparticles hybrid for efficient electrophoresis separation of amino acids

Abstract

In this paper, a novel, simple, economical and environmentally friendly method based on in situ chemically induced synthesis strategy was designed and developed for the modification of a poly(dimethylsiloxane) (PDMS) microchip channel with polydopamine/gold nanoparticles (PDA/Au NPs) to create a hydrophilic and biofouling resistant surface. Dopamine as a reductant and a monomer, and HAuCl(4) as an oxidant to trigger dopamine polymerization and the source of metallic nanoparticles, were filled into the PDMS microchannel to yield in situ a well-distributed and robust PDA/Au NP coating. Au NPs were highly and uniformly dispersed in/on the PDA matrix with a narrow size distribution, as verified by scanning electron microscopy and UV-vis spectra. Compared with the native PDMS microchannel, the modified surfaces exhibited much better wettability, high stability and suppressed electroosmotic mobility, and less nonspecific adsorption towards biomolecules. The water contact angle and EOF of PDA/Au NP-coated PDMS microchip were measured to be 13° and 4.17×10(-4) cm(2)/V s, compared to those of 111° and 5.33×10(-4) cm(2)/V s from the native one, respectively. Fast and efficient separations of five amino acids such as arginine, proline, histidine, valine and threonine suggested greatly improved electrophoretic performance of the PDA/Au NP-functionalized PDMS microchips. This one-step procedure offers an effective approach for a biomimetic surface design on microfluidic chips, which is promising in high-throughput and complex biological analysis.

One‐step preparation and application of mussel‐inspired poly(norepinephrine)‐coated polydimethylsiloxane microchip for separation of chiral compounds

“…PDMS microchip with a simple cross‐configuration was fabricated as previously described procedure . Briefly, PDMS oligomer and curing agent were thoroughly mixed at a ratio of 10:1 w/w, degassed, and then poured on the SU‐8 2035 photoresist molding.…”

Applications of polydopamine modifications in capillary electrophoretic analysis

“…(B) Separation mechanism of the electrophoretic microchip system for five amino acids analysis integrated with end‐channel electrochemical detection. Reprinted with permission from …”

Hierarchical bioceramic scaffolds with 3D-plotted macropores and mussel-inspired surface nanolayers for stimulating osteogenesis

“…The formed polydopamine contains a large number of bioactive groups including catechol moieties, -OH and -NH 2 and therefore can bind strongly to different kinds of materials 14,17,18 . It has been found that the formed polydopamine nanolayer on the surface of many materials, such as noble metals, oxides, semiconductors and various synthetic polymers could improve the apatitemineralization ability and cell attachments [19][20][21][22][23] DAPI solution (5 mg mL -1 ) was added to counterstain the cell nuclei.…”