Pectin methylesterase inhibitor

Giovane, Alfonso; Servillo, Luigi; Balestrieri, Ciro; Raiola, Alessandro; D’Avino, Rossana; Tamburrini, Maurizio; Ciardiello, Maria Antonietta; Camardella, Laura

doi:10.1016/j.bbapap.2003.08.011

Cited by 171 publications

(134 citation statements)

References 54 publications

Supporting

Mentioning

130

Contrasting

Order By: Relevance

“…Analysis using neural networks and hidden Markov models hosted at SignalP server suggested that the ORF of MaPMEI comprised of an N-terminal 26 amino acid residues of a signal peptide. This region corresponds with the positions in other PMEIs, which is required for the extracellular targeting of the protein at the site of its function (Giovane et al 2004). Protein sequence alignment and subsequent in silico analyses revealed the presence of all the four conserved cystine residues corresponding to other PMEIs and invertase inhibitors from other sources (Fig.…”

Section: Resultsmentioning

confidence: 85%

“…These PME isoforms are encoded by a family of genes in dicot plants (Giovane et al 1994), which may be expressed in constitutive or in differential manner depending upon the developmental stage and specific tissue (Gaffe et al 1997). Furthermore, ripe fruits are more susceptible to phytopathogenic fungi and bacteria that also produce PME isoforms (Giovane et al 2004), higher expression of MaPMEI even at later stages of ripening, may also be playing a role in controlling the non-host PMEs. Although, increase in PME activity during ripening in banana (Lohani et al 2004) and high level of continued expression of MaPMEI specifically in fruit strongly suggests the regulation of PMEs also occurs at post-translational level, however, the exact mechanism and level of specificity for PME isoforms needs to be demonstrated using the biochemical and structural biology approach.…”

Section: Resultsmentioning

confidence: 99%

“…Activity of PME is regulated by mechanisms such as differential expression of isoforms at transcriptional level and formation of complex with inhibitory proteins at post-translational level (Balestrieri et al 1990;Camardella et al 2000;Jolie et al 2010;Vandevenne et al 2011). Among these, PME/Invertase inhibitor has been shown to regulate the activity of PME at post-translational level by binding to these proteins in a non-covalent 1:1 manner (Giovane et al 2004;Jolie et al 2009). The inhibitors of PME and Invertase are unique as they share very similar sequence and topology.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Isolation and characterization of ripening related pectin methylesterase inhibitor gene from banana fruit

Srivastava

Gupta

Sane

et al. 2012

Physiol Mol Biol Plants

View full text Add to dashboard Cite

Identification of ethylene-regulated and ripeningrelated genes from banana (Musa acuminata Var. Harichaal) fruits using DDRT-PCR led to the isolation of differentially expressed partial cDNA of pectin methylesterase inhibitor (MaPMEI) gene. Its full-length cDNA sequence consisted of a 567 bp ORF, encoding a protein of 189 aa with deduced molecular mass 19.6 kDa. Expression pattern of MaPMEI gene revealed that upon ethylene treatment, this gene is upregulated initially giving maximum expression in postclimacteric stage then decreases slightly in later stages of ripening. 1-MCP, a known ethylene perception inhibitor, inhibits both fruit ripening as well as the transcript level of this gene. Also, the transcripts of MaPMEI gene were not detected during the short time ethylene treatment suggesting this gene appears to be not directly induced by ethylene. Interestingly, MaPMEI gene showed fruit specific expression that indicates its possible role in the regulations of PMEs in fruits. In silico analysis revealed a predicted signal peptide sequence necessary for localization of MaPMEI in the cell wall. Furthermore, the four Cys residues involved in disulfide bridges are conserved in MaPMEI similar to other PMEIs and invertase inhibitors. Phylogenetic analysis further suggests that the MaPMEI identified in this study is more closely related to PMEIs than to invertase inhibitors.

show abstract

Section: Resultsmentioning

confidence: 85%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Isolation and characterization of ripening related pectin methylesterase inhibitor gene from banana fruit

Srivastava

Gupta

Sane

et al. 2012

Physiol Mol Biol Plants

View full text Add to dashboard Cite

show abstract

“…Small molecule inhibitors would be more tractable as applied enzyme inhibitors. Several proteinaceous inhibitors of pectin methyl esterase (termed PMEIs) have been identified (D'Avino et al, 2003;Giovane et al, 2004;Juge, 2006;Raiola et al, 2004;Wolf et al, 2003). However, to our knowledge, no small molecules have been implicated in inhibition of PME to date.…”

Section: Introductionmentioning

confidence: 99%

Inhibition of pectin methyl esterase activity by green tea catechins

Lewis¹,

Selzer

Shahar

et al. 2008

Phytochemistry

View full text Add to dashboard Cite

“…Targeting to the endomembrane system leads to the export of PMEs into the cell wall and is mediated either by a common type signal peptide or a transmembrane domain (signal anchor). The pro-region (Pfam 04043) shares similarities with the PMEI domain and is thought to play an autoinhibitory role during the enzyme's secretion into the apoplast, where only the mature part of the protein is found (Giovane et al, 2004 The deduced amino acid sequences of C. arabica and other known plant pectin methyesterases were aligned, and a phylogenetic tree was drawn to classify Coffea PMEs (Figure 1). Phylogenetic analysis showed that Coffea PMEs are distributed in 5 of the 9 clades assigned for plant PMEs (Markovic and Janecek, 2004).…”

Section: In Silico Analysismentioning

confidence: 99%

Gene expression and enzymatic activity of pectin methylesterase during fruit development and ripening in Coffea arabica L.

Cação

Leite

Budzinski³

et al. 2012

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. Coffee quality is directly related to the harvest and post harvest conditions. Non-uniform maturation of coffee fruits, combined with inadequate harvest, negatively affects the final quality of the product. Pectin methylesterase (PME) plays an important role in fruit softening due to the hydrolysis of methylester groups in cell wall pectins. In order to characterize the changes occurring during coffee fruit maturation, the enzymatic activity of PME was measured during different stages of fruit ripening. PME activity progressively increased from the beginning of the ripening process to the cherry fruit stage. In silico analysis of expressed sequence tags of the Brazilian Coffee Genome Project database identified 5 isoforms of PME. We isolated and cloned a cDNA homolog of PME for further characterization. CaPME4 transcription was analyzed in pericarp, perisperm, and endosperm tissues during fruit development and ripening as well as in other plant tissues. Northern blot analysis revealed increased transcription of CaPME4 in the pericarp 300 days after flowering. Low levels of CaPME4 mRNAs were observed in the endosperm 270 days after flowering. Expression of CaPME4 transcripts was strong in the branches and lower in root and flower tissues. We showed that CaPME4 acts specifically during the later stages of fruit ripening and possibly contributes to the softening of coffee fruit, thus playing a significant role in pectin degradation in the fruit pericarp.

show abstract

Pectin methylesterase inhibitor

Cited by 171 publications

References 54 publications

Isolation and characterization of ripening related pectin methylesterase inhibitor gene from banana fruit

Isolation and characterization of ripening related pectin methylesterase inhibitor gene from banana fruit

Inhibition of pectin methyl esterase activity by green tea catechins

Gene expression and enzymatic activity of pectin methylesterase during fruit development and ripening in Coffea arabica L.

Contact Info

Product

Resources

About