Pectobacterium and Dickeya: Environment to Disease Development

Toth, Ian K.; Barny, Marie‐Anne; Brurberg, May Bente; Condemine, Guy; Czajkowski, Robert; Elphinstone, J. G.; Hélias, Valerie; Johnson, Steven B.; Moleleki, Lucy N.; Pirhonen, Minna; Rossmann, Simeon; Tsror, Leah; Waals, J. E. van der; Wolf, J.M. van der; Gijsegem, Frédérique Van; Yedidia, Iris

doi:10.1007/978-3-030-61459-1_3

“…SRP are ubiquitous by nature and can be found in different ecological niches such as soil, water and the host (Charkowski et al, 2012). Nonetheless, it has to be noted that survival outside the host for the SRP is only for short periods of time (Toth et al, 2021). Hence, the ability (conferred by MCPs as demonstrated here) to sense and move toward the host enables them to move from unfavorable niches to the host environment where survival is more likely (Van Gijsegem et al, 2021).…”

Section: Discussionmentioning

confidence: 80%

Pectobacterium brasiliense 1692 Chemotactic Responses and the Role of Methyl-Accepting Chemotactic Proteins in Ecological Fitness

Tanui

¹

,

Shyntum

²

,

Sedibane

³

et al. 2021

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To adapt to changing environmental niches, bacteria require taxis, a movement toward or away from a stimulus (ligand). Chemotaxis has been studied in some members of the Soft Rot Pectobacteriaceae (SRP), particularly members of the genus Dickeya. On the contrary, there are fewer studies on this topic for the other genus in the SRP group, namely Pectobacterium. This study evaluated chemotactic responses in Pectobacterium brasiliense (Pb 1692) to various ligands. A total of 34 methyl-accepting chemotactic proteins (MCPs) were identified in the Pb 1692 genome and the domain architectures of these MCPs were determined. Four Pb 1692 MCPs previously shown to be differentially expressed during potato tuber infection were selected for further functional characterization. Toward this end, Pb 1692 mutant strains each lacking either AED-0001492, AED-0003671, AED-0000304, or AED-0000744 were generated. Two of these mutants (AED-0001492 and AED-0003671), were attenuated in their ability to grow and respond to citrate and are thus referred to as MCPcit2 and MCPcit1, respectively, while the other two, AED-0000304 (MCPxyl) and AED-0000744 (MCPasp), were affected in their ability to respond to xylose and aspartate, respectively. Trans-complementation of the mutant strains restored swimming motility in the presence of respective ligands. The four MCP mutants were not affected in virulence but were significantly attenuated in their ability to attach to potato leaves suggesting that ecological fitness is an important contribution of these MCPs toward Pb 1692 biology.

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“…Another important area where qPCR detection of SRP is needed is the establishment of the threshold bacterial population necessary for the development of disease symptoms. While the occurrence of SRP-related blackleg, wilting and aerial rot of vegetating potato depends on numerous environmental factors (reviewed in [ 39 ]), the development of soft rot in stored ware and seed potato is a consequence of a latent infection of the tuber surface. The incidence of soft rot, as a minimum, correlates with the population of SRP as revealed by laboratory testing.…”

Section: Discussionmentioning

confidence: 99%

Quantitative Real-Time PCR Assay for the Detection of Pectobacterium parmentieri, a Causal Agent of Potato Soft Rot

Lukianova

¹

,

Evseev

²

,

Stakheev

³

et al. 2021

Plants

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Pectobacterium parmentieri is a plant-pathogenic bacterium, recently attributed as a separate species, which infects potatoes, causing soft rot in tubers. The distribution of P. parmentieri seems to be global, although the bacterium tends to be accommodated to moderate climates. Fast and accurate detection systems for this pathogen are needed to study its biology and to identify latent infection in potatoes and other plant hosts. The current paper reports on the development of a specific and sensitive detection protocol based on a real-time PCR with a TaqMan probe for P. parmentieri, and its evaluation. In sensitivity assays, the detection threshold of this protocol was 102 cfu/mL on pure bacterial cultures and 102–103 cfu/mL on plant material. The specificity of the protocol was evaluated against P. parmentieri and more than 100 strains of potato-associated species of Pectobacterium and Dickeya. No cross-reaction with the non-target bacterial species, or loss of sensitivity, was observed. This specific and sensitive diagnostic tool may reveal a wider distribution and host range for P. parmentieri and will expand knowledge of the life cycle and environmental preferences of this pathogen.

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“…zeae complex are major pathogens responsible for the maize stalk rot and rice foot rot diseases [18]. They have the ability to infect monocot but also dicot plants like potato [2, 19, 20] and they are commonly found in waterways [21, 22]. Fourteen genomes of strains belonging to the D.…”

Section: Introductionmentioning

confidence: 99%

“…The genus Dickeya comprises enterobacteral plant pathogens, which cause disease in a large range of economically important crops and ornamentals, and environmental strains isolated from water [1, 2]. This genus was defined in 2005 by the reclassification of former Erwinia chrysanthemi into six species: Dickeya chrysanthemi , Dickeya dadantii , Dickeya diffenbachiae , Dickeya dianthicola , Dickeya zeae and Dickeya paradisiaca [3].…”

Section: Introductionmentioning

confidence: 99%

Diversity within the Dickeya zeae complex, identification of Dickeya zeae and Dickeya oryzae members, proposal of the novel species Dickeya parazeae sp. nov.

Hugouvieux‐Cotte‐Pattat

¹

,

Gijsegem

²

2021

International Journal of Systematic and Evolutionary Microbiology

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The genus Dickeya comprises plant pathogens that cause diseases in a large range of economically important crops and ornamentals. Strains previously assigned to the species Dickeya zeae are major pathogens attacking vital crops such as maize and rice. They are also frequently isolated from surface water. The newly described species Dickeya oryzae is closely related to D. zeae members, so that the limit between the two species can be difficult to define. In order to clearly distinguish the two species, globally described by the term ‘ D. zeae complex’, we sequenced the genome of four new water isolates and compared them to 14 genomes available in databases. Calculation of average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values confirmed the phylogenomic classification into the two species D. zeae and D. oryzae . It also allowed us to propose a new species, Dickeya parazeae sp. nov., to characterize a clade distinct from those containing the D. zeae type strain NCPPB2538T. Strain S31T (CFBP 8716T=LMG 32070T) isolated from water in France is proposed as the type strain of the new species. Phenotypic analysis of eight publically available strains revealed traits common to the five tested D. oryzae members but apparently not shared by the D. oryzae type strain. Genomic analyses indicated that a simple distinction between the species D. zeae , D. parazeae and D. oryzae can be obtained on the basis of the recA sequence. D. oryzae can be distinguished from the two other species by growth on l-tartaric acid. Based on the recA marker, several strains previously identified as D. zeae were re-assigned to the species D. parazeae or D. oryzae . This study also highlighted the broad host range diversity of these three species.

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Pectobacterium and Dickeya: Environment to Disease Development

Cited by 47 publications

References 199 publications

Pectobacterium brasiliense 1692 Chemotactic Responses and the Role of Methyl-Accepting Chemotactic Proteins in Ecological Fitness

Pectobacterium brasiliense 1692 Chemotactic Responses and the Role of Methyl-Accepting Chemotactic Proteins in Ecological Fitness

Quantitative Real-Time PCR Assay for the Detection of Pectobacterium parmentieri, a Causal Agent of Potato Soft Rot

Diversity within the Dickeya zeae complex, identification of Dickeya zeae and Dickeya oryzae members, proposal of the novel species Dickeya parazeae sp. nov.

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