Pectobacterium carotovorum subsp. actinidiae associated with canker on pear trees in Brazil

Araújo, Leonardo; Cardoza, Yuliet Franco; Duarte, Valmir; Moraes, Marcelo Gravina de

doi:10.1007/s10658-020-02123-5

Cited by 3 publications

(4 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These strains were obtained from a total of 52 samples collected across 38 distinct growing areas. The physiological and biochemical features were used as additional methods for the differentiation of Pectobacterium species (Loc et al., 2022), which aligned with findings from previously characterized Pectobacterium species (Araujo et al., 2021). The strains exhibited typical attributes associated with Pectobacterium taxa, including the formation of cavities on CVP plates, solubility in KOH (rendering them gram negative), facultative anaerobic metabolism, a positive catalase test, and a lack of fluorescence under UV light on KB medium (Charkowski et al., 2020; Czajkowski et al., 2015; Koh et al., 2012).…”

Section: Discussionmentioning

confidence: 78%

“…Subsequent reports highlighted its role in causing bacterial canker on kiwifruit across various provinces in China (Lu et al, 2020;Wu et al, 2017;Yan et al, 2019;Zhang et al, 2023) and its linkage to canker on pear trees in Brazil (Araujo et al, 2021) and South Korea (Choi et al, 2022). Contrary to the initial report, recent research by Portier et al (2019) indicates that isolate C331 (CFBP 7370), originally obtained from soft-rotted potatoes in Syria in 2004, had been inaccurately identified as P. carotovorum (Nabhan et al, 2012) and has since been reclassified as P. actinidiae.…”

Section: P Carotovorum Was Identified In Dieffenbachia Plants In the ...mentioning

confidence: 99%

“…Subsequent reports highlighted its role in causing bacterial canker on kiwifruit across various provinces in China (Lu et al., 2020; Wu et al., 2017; Yan et al., 2019; Zhang et al., 2023) and its linkage to canker on pear trees in Brazil (Araujo et al., 2021) and South Korea (Choi et al., 2022). Contrary to the initial report, recent research by Portier et al.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Isolation, identification, and characterization of Pectobacterium actinidiae isolated from infected sugar beet leaves

Öztürk

2024

Journal of Phytopathology

View full text Add to dashboard Cite

During the sugar beet growing seasons of 2019 and 2020, symptomatic leaf samples displaying necrotic spots were collected for the isolation of pectinolytic bacteria. Seven bacterial isolates were obtained from these infected leaves on crystal violet pectate (CVP) medium. Two of these isolates exhibited the ability to macerate potato tuber slices and induced disease symptoms on petioles of sugar beet plants. Initially, their identity was determined to be Pectobacterium spp. through a polymerase chain reaction (PCR) that produced a 434 bp amplicon using Y1/Y2 primers. Subsequently, they generated a 550 bp PCR product with the EXPCCF/EXPCCR primers, classifying them as P. carotovorum. Further analysis of both strains involved the examination of the dnaX and mdh genes to confirm their taxonomic status. BLASTn analysis revealed the highest sequence similarity with P. actinidiae strains in GenBank for dnaX (99.39%–100%) and mdh (100%) genes. Phylogenetic analysis, based on sequences from dnaX, mdh, and a concatenation of both genes, conducted alongside 21 species of Pectobacterium spp., unequivocally confirmed that strains Pact1 and Pact2 belong to P. actinidiae. This study marks the first‐ever report of the presence of P. actinidiae in sugar beet worldwide.

show abstract

Section: Discussionmentioning

confidence: 78%

Section: P Carotovorum Was Identified In Dieffenbachia Plants In the ...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Isolation, identification, and characterization of Pectobacterium actinidiae isolated from infected sugar beet leaves

Öztürk

2024

Journal of Phytopathology

View full text Add to dashboard Cite

show abstract

“…In the field, the success of this method is highly dependent on the occurrence of the susceptible blossom tissues and weather conditions suitable for sufficient disease pressure (Kostick et al 2019). The inoculation of detached shoots as a cheap fast-phenotyping method for pear susceptibility to bacterial pathogens rather than whole trees would speed up this process (Araujo et al 2021). Differences in the virulence of EA strains are common, but a strain is expected to have the same level of virulence in all pear cultivars.…”

mentioning

confidence: 99%

Comparison of the shoot and blossom susceptibility of European and Asian pear cultivars to fire blight across different conditions

Pánková¹,

Krejzar²,

Buchtová³

et al. 2023

Plant Prot. Sci.

View full text Add to dashboard Cite

The susceptibility of 14 pear cultivars to the bacterium Erwinia amylovora, the causal agent of fire blight disease, was evaluated using three different methods of in vivo inoculation -the leaf inoculation method on detached shoots of pear cultivars in a climate chamber, and the terminal shoot and blossom inoculation methods on potted trees of the same pear cultivars in a net house. The results of the artificial inoculations of the European and Asian pear cultivars were compared using the susceptibility score scale, as well as the ANOVA and post hoc methods. The differences among the pear cultivar susceptibility increased during the evaluation periods in the given year, but there were no significant differences between the annual evaluation in 2020 and 2021. The results of the pear cultivar susceptibility after inoculation of the detached shoots differed significantly from the results of the terminal shoot and blossom inoculation. The detached shoot susceptibility under pathogen-favourable conditions in the climate chamber was lower than the terminal shoot and blossom susceptibility in the net house for most pear cultivars. In both years, the highest values of the terminal shoot and blossom susceptibility were coincidently calculated for the Ananaska česká European old cultivar and the Nijissejkii Asian cultivar.

show abstract

First Report of Canker Caused by Pectobacterium actinidiae on Asian Pear Trees (Pyrus pyrifolia) in South Korea

Choi¹,

Song²,

Park

2023

Plant Disease

View full text Add to dashboard Cite

In July 2020, pear trees (Pyrus pyrifolia cv. Niitaka) with cankers displaying dark-red bacterial ooze on the trunk and branches were found in two pear orchards located in Naju, Jeollanam-do, South Korea (34°57′50″ N, 126°43′52″ E and 34°56′14″ N, 126°33′42″ E). The incidence was 1.5% (3 out of 200 trees) and 0.83% (1 out of 120 trees), respectively. The symptoms were similar to those of the bleeding canker caused by Dickeya fangzhongdai (Choi et al. 2021), which is typically observed in October. The bacterial ooze was suspended in sterile water and streaked in Luria–Bertani (LB) medium to isolate single bacterial colonies. Two isolates (PRI-B16 and PRI-B17) from representative diseased trees were selected for investigation. Physiological and biochemical characteristics of the isolates analyzed using the BIOLOG GEN III MicroPlate™ system (Biolog, Hayward, CA, USA) were similar to the characteristics of Pectobacterium actinidiae (Portier et al. 2019). These isolates were positively utilized stachyose, L-galactonic acid-g-lactone, guanidine hydrochloride and weakly utilized (-)-D-arabitol (Portier et al. 2019). Bacterial genomic DNA was extracted from cell cultured in 5 ml LB at 28C for 2 days using G-spin DNA extraction kit (iNtRON Biotechnology, Korea) according to the manufacturer’s protocol. PCR amplification was amplified as Portier et al. (2019). The generated their sequences of the small subunit ribosomal RNA (16S rRNA) using primers 27f and 1492r (Heuer et al. 1997) (Genebank accession numbers: ON951863 and ON951864) were 99.86% and 99.76% identical, respectively, to that of P. actinidiae isolate SCPJ-1 (KY307837.1) by a BLAST search against gene bank databases. The dnaX (Genebank accession nos: ON960281 and ON960282), leuS (Genebank accession nos: ON960283 and ON960284), and recA (Genebank accession nos: ON960285 and ON960286) genes of these isolates were also amplified and sequenced by previously described Stawiak et al. (2009) for dnaX and leuS, and Waleron et al. (2002) for recA. A neighbor-joining phylogenetic analysis based on the concatenated dnaX, leuS, and recA sequences placed the two isolates in a clade containing previously identified P. actinidiae isolates. A pathogenicity test was conducted using two-year-old pear (P. pyrifolia cv. Nittaka) trees grown in a greenhouse. Wounded and unwounded pear tree branches were inoculated with 10 µL of the bacterial suspension (108 CFU/ml) or sterile water as a control. The inoculated plants were maintained at 30°C without light for 2 days under 85-90% humidity. At 7 days post-inoculation, bacterial ooze was observed on the branches inoculated with a bacterial suspension, whereas branches subjected to unwounded inoculation and water inoculation exhibited no symptoms. This assay was performed three times. We reisolated two colonies from each sample showing typical bleeding symptoms and confirmed their identity by sequencing the dnaX locus. Pectobacterium actinidiae has been reported to cause canker in pear trees in Brazil (Araujo et al. 2021) as well as kiwifruit in South Korea (Koh et al. 2012). This is the first report of P. actinidiae causing canker on pear trees in South Korea and is, therefore, pathologically significant.

show abstract

Pectobacterium carotovorum subsp. actinidiae associated with canker on pear trees in Brazil

Cited by 3 publications

References 17 publications

Isolation, identification, and characterization of Pectobacterium actinidiae isolated from infected sugar beet leaves

Isolation, identification, and characterization of Pectobacterium actinidiae isolated from infected sugar beet leaves

Comparison of the shoot and blossom susceptibility of European and Asian pear cultivars to fire blight across different conditions

First Report of Canker Caused by Pectobacterium actinidiae on Asian Pear Trees (Pyrus pyrifolia) in South Korea

Contact Info

Product

Resources

About