Pectobacterium zantedeschiae sp. nov. a new species of a soft rot pathogen isolated from Calla lily (Zantedeschia spp.)

Waleron, Małgorzata; Misztak, Agnieszka; Franczuk, Martyna; Jońca, Joanna; Wielgomas, Bartosz; Mikiciński, Artur; Popović, Tatjana; Waleron, Krzysztof

doi:10.1016/j.syapm.2018.08.004

Cited by 50 publications

(40 citation statements)

References 46 publications

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“…'Pectobacterium peruviense' and 'Pectobacterium zantedeschiae' [27,28]. Given the taxonomic evolution in the past ten years, the ecological importance, repartition and habitat of most species need to be evaluated.…”

Section: Introductionmentioning

confidence: 99%

Updated Taxonomy of Pectobacterium genus in the CIRM-CFBP Bacterial Collection: When Newly Described Species Reveal “Old” Endemic Population

Portier¹,

Pédron²,

Taghouti³

et al. 2020

Preprint

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Bacterial collections are invaluable tools for microbiologists. However, their practical use is compromised by imprecise taxonomical assignation of bacterial strains. This is particularly true for soft rotting plant pathogens of the Pectobacterium genus. To solve this difficulty, we analyzed the taxonomic status of 265 Pectobacterium strains deposited at CIRM-CFBP collection from 1944 to 2020. This collection gathered Pectobacterium strains isolated in 27 countries from 32 plant species representing 17 botanical families or from non-host environments. MLSA approach completed by genomic analysis of 15 strains was performed to update the taxonomic status of these 265 strains. Results showed that the CIRM-CFBP Pectobacterium collection harboured at least one strain of each species to the exception of P. polonicum. Yet, 6 strains could not be assigned to any of the described species and may represent at least two new species. Surprisingly, the P. versatile species, recently described in 2019, is the most prevalent species among CIRM-CFBP strains. Analysis of P. versatile strains revealed that this species is endemic all over the world on various host plants and environments. At the opposite, other species gathered strains isolated from only one botanical family or exclusively from fresh water environment. Our work also revealed new host plants for several Pectobacterium spp.

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Section: Introductionmentioning

confidence: 99%

Updated Taxonomy of Pectobacterium genus in the CIRM-CFBP Bacterial Collection: When Newly Described Species Reveal “Old” Endemic Population

Portier¹,

Pédron²,

Taghouti³

et al. 2020

Preprint

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show abstract

“…These were reported to be more virulent on monocot plants than on dicot plants, presented typical amplified fragment length polymorphism (AFLP) profiles and distinct banding pattern in intergenic transcribed spacer (ITS) PCR analysis [ 13 ]. The combination of molecular patterns with MLSA and recombinase polymerase amplification analyses indicated that these monocot isolates represent multiple species in the genus, P. aroidearum, P. zantedeschiae, P. betavasculorum, and P. wasabiae [ 12 , 14 , 15 ]. Atypical infections have also been reported in other monocots including Allium cepa [ 16 ], Zantedeschia spp.…”

Section: Introductionmentioning

confidence: 99%

Host Specificity and Differential Pathogenicity of Pectobacterium Strains from Dicot and Monocot Hosts

et al. 2020

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Recent phylogenetic studies have transferred certain isolates from monocot plants previously included in the heterogeneous group of Pectobacteriumcarotovorum (Pc) to a species level termed Pectobacterium aroidearum. The specificity of Pectobacterium associated infections had received less attention, and may be of high scientific and economic importance. Here, we have characterized differential responses of Pectobacterium isolates from potato (WPP14) and calla lily (PC16) on two typical hosts: Brassica oleracea var. capitata (cabbage) a dicot host; and Zantedeschia aethiopica (calla lily) a monocot host. The results revealed clear host specific responses following infection with the two bacterial strains. This was demonstrated by differential production of volatile organic compounds (VOCs) and the expression of plant defense-related genes (pal, PR-1, lox2, ast). A related pattern was observed in bacterial responses to each of the host’s extract, with differential expression of virulence-related determinants and genes associated with quorum-sensing and plant cell wall-degrading enzymes. The differences were associated with each strain’s competence on its respective host.

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“…The genus Pectobacterium comprises the following species: Pectobacterium carotovorum, Pectobacterium atrosepticum, Pectobacterium betavasculorum, Pectobacterium cacticida, Pectobacterium aroidearum (Nabhan et al 2013), Pectobacterium wasabiae, Pectobacterium parmentieri (Khayi et al 2016), Pectobacterium polaris (Dees et al 2017), Pectobacterium peruviense (Waleron et al 2018), and Pectobacterium punjabense (Sarfraz et al 2018). In addition, Pectobacterium zantedeschiae (Waleron et al 2019a), Pectobacterium aquaticum (Pedron et al 2019), Pectobacterium fontis (Oulghazi et al 2019), Pectobacterium polonicum (Waleron et al 2019b), and the new genomospecies, Candidatus Pectobacterium maceratum (Shirshikov et al 2018;Waleron et al 2019c) are recently added to the genus. P. carotovorum isolates are subsequently divided into three different subspecies viz.…”

Section: Introductionmentioning

confidence: 99%

A novel subspecies-specific primer targeting the gyrase B gene for the detection of Pectobacterium carotovorum subsp. brasiliense

2019

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Abstract. Joko T, Soffan A, Muhammad Saifur Rohman MS. 2019. A novel subspecies-specific primer targeting the gyrase B gene for the detection of Pectobacterium carotovorum subsp. brasiliense. Biodiversitas 20: 3042-3048. Pectobacterium carotovorum subsp. brasiliense is one of the major causative bacterial pathogens of the soft rot disease in various crops. It has a high virulence and a wide range of hosts in the tropics and the subtropics. Most often, conventional methods are not able to accurately distinguish P. carotovorum subsp. brasiliense from other subspecies. Thus, this study aimed to design a specific gyrase B gene (gyrB) -based primers for the detection and identification of soft rot pathogen. The specific primers design was based on the alignment using gyrB gene sequence data from P. carotovorum subsp. brasiliense and other data from the GenBank. The primers comprised of F-gyr-Pcb (5’-CAC AGG CAC CGC TGG CTG TT-3’) and R-gyr-Pcb (5’-CGT CGT TCC ACT GCA ATG CCA-3’) with an amplicon of 336 base pairs. The specificity of the primers pair was verified both in silico and in polymerase chain reaction (PCR) assays, where the primers could only detect P. carotovorum subsp. brasiliense. Primers’ sensitivity was determined by qualitative PCR with a detection limit of less than 0.5 ng/µL of genomic DNA. Hence, the proposed detection tool can be beneficial to advance further studies on the ecology and epidemiology of soft rot diseases.

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Pectobacterium zantedeschiae sp. nov. a new species of a soft rot pathogen isolated from Calla lily (Zantedeschia spp.)

Cited by 50 publications

References 46 publications

Updated Taxonomy of Pectobacterium genus in the CIRM-CFBP Bacterial Collection: When Newly Described Species Reveal “Old” Endemic Population

Updated Taxonomy of Pectobacterium genus in the CIRM-CFBP Bacterial Collection: When Newly Described Species Reveal “Old” Endemic Population

Host Specificity and Differential Pathogenicity of Pectobacterium Strains from Dicot and Monocot Hosts

A novel subspecies-specific primer targeting the gyrase B gene for the detection of Pectobacterium carotovorum subsp. brasiliense

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