Peer Review #2 of "Expression patterns and the prognostic value of the EMILIN/Multimerin family members in low-grade glioma (v0.2)"

Abstract

The management of Low-grade glioma (LGG) remains a challenge because of the infiltrating nature of the tumor, which cannot be cured by surgical resection. The EMILIN/Multimerins containing the gC1q signature which were involved in many processes of tumor development. However, the expression of EMILIN/Multimerins in LGG and its prognostic value remains unclear. This study investigates the expression pattern, prognostic significance and function of EMILIN/Multimerins in LGG patients based on integrated bioinformatics analysis. The transcriptional levels and prognosis of EMILIN/Multimerins in LGG were analyzed by the ONCOMINE, GEPIA and UALCAN. The EMILIN/Multimerins' mutation, co-expression neighboring genes were analyzed via cBioProtal. TIMER and Metascape were used to reveal the potential mechanism of EMILIN/Multimerins in LGG. And we found that most EMILIN/Multimerins were overexpressed in LGG and share a clear association with immune cells. GEPIA confirmed that high levels of EMILIN/Multimerins were closely correlated with poor prognosis in LGG patients, except the MMRN2 in disease-free survival (DFS). The expression of EMILIN/Multimerins was related to different pathological grades. Potential mechanisms of different EMILIN/Multimerins in regulating LGG was revealed. Our findings showed that EMILIN/Multimerins might serve as novel prognostic biomarkers and possibly be a highpriority therapeutic target for LGG patients. PeerJ reviewing PDF | Abstract 24 Managing low-grade gliomas (LGG) remains a major medical challenge due to the infiltrating 25 nature of the tumor and failure of surgical resection to eliminate the disease. EMILIN/Multimerins 26 containing the gC1q signature which are involved in many tumor processes. However, the 27 expression and prognostic value of EMILIN/Multimerins in LGG remains unclear. This study used 28 used integrated bioinformatics analysis to investigates the expression pattern, prognostic value and 29 function of EMILIN/Multimerins in patients with LGG.30We analyzed the transcription levels and prognostic value EMILIN/Multimerins in LGG using 31 the ONCOMINE, GEPIA (Gene Expression Profiling Interactive Analysis) 32 and UALCAN databases. The mutation and co-expression rates of neighboring genes in 33 EMILIN/Multimerins were studied using cBioPortal. TIMER and Metascape were used to reveal 34 the potential function of EMILIN/Multimerins in LGG. 35According to our analysis, most EMILIN/Multimerins were overexpressed in LGG and shared 36 a clear association with immune cells. GEPIA analysis confirmed that high levels of 37 EMILIN/Multimerins, not including MMRN2, were associated with a poor prognosis in disease-38 free survival (DFS) of patients with LGG. Additionally, we discovered that EMILIN/Multimerins 39 may regulate LGG and we found a correlation between their expression patterns and distinct 40 pathological grades. 41 We found that EMILIN/Multimerins serve as possible prognostic biomarkers and high-priority 42 therapeutic targets patients with LGG.