PEG-b-PPA/DNA micelles improve transgene expression in rat liver through intrabiliary infusion

Jiang, Xiang; Dai, Hui; Ke, Chyan Ying; Mo, Xinxin; Torbenson, Michael; Li, Zhiping; Mao, Hai‐Quan

doi:10.1016/j.jconrel.2007.06.014

Cited by 41 publications

(47 citation statements)

References 36 publications

(54 reference statements)

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“…AFM micrographs of 3, coincubated with pBR322 plasmid DNA, revealed formation of irregular shaped structures suggesting interaction of DNA with carrier molecules as previously reported for DNA-micelle complexation [27] (Fig. 4A).…”

Section: Resultssupporting

confidence: 82%

Mannosylated self-assembled structures for molecular confinement and gene delivery applications

Gour

Purohit

Verma

et al. 2009

Biochemical and Biophysical Research Communications

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Section: Resultssupporting

confidence: 82%

Mannosylated self-assembled structures for molecular confinement and gene delivery applications

Gour

Purohit

Verma

et al. 2009

Biochemical and Biophysical Research Communications

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“…Chitosan and PEG-b-PPA were chosen because both had previously been shown to promote transgene expression while forming nanocomplexes. 454 In the fully formed complex, ET (E 12 ) proceeded from the QD donor (1) to the Cy3/BOBO-3 acceptors (2) and then (E 23 ) from Cy3/BOBO-3 (2) to Cy5 (3) as monitored by single particle characterization. Changes in FRET were used to monitor unpacking (reduction in E 23 ) and DNA degradation (reduction in E 12 ) within the complex.…”

Section: Kinases and Phosphatasesmentioning

confidence: 99%

Energy Transfer with Semiconductor Quantum Dot Bioconjugates: A Versatile Platform for Biosensing, Energy Harvesting, and Other Developing Applications

Spillmann²,

et al. 2016

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Luminescent semiconductor quantum dots (QDs) are one of the more popular nanomaterials currently utilized within biological applications. However, what is not widely appreciated is their growing role as versatile energy transfer (ET) donors and acceptors within a similar biological context. The progress made on integrating QDs and ET in biological configurations and applications is reviewed in detail here. The goal is to provide the reader with (1) an appreciation for what QDs are capable of in this context, (2) how this field has grown over a relatively short time span, and, in particular, (3) how QDs are steadily revolutionizing the development of new biosensors along with a myriad of other photonically active nanomaterial-based bioconjugates. An initial discussion of QD materials along with key concepts surrounding their preparation and bioconjugation is provided given the defining role these aspects play in the QDs ability to succeed in subsequent ET applications. The discussion is then divided around the specific roles that QDs provide as either Förster resonance energy transfer (FRET) or charge/electron transfer donor and/or acceptor. For each QD-ET mechanism, a working explanation of the appropriate background theory and formalism is articulated before examining their biosensing and related ET utility. Other configurations such as incorporation of QDs into multistep ET processes or use of initial chemical and bioluminescent excitation are treated similarly. ET processes that are still not fully understood such as QD interactions with gold and other metal nanoparticles along with carbon allotropes are also covered. Given their maturity, some specific applications ranging from in vitro sensing assays to cellular imaging are separated and discussed in more detail. Finally a perspective on how this field will continue to evolve is provided.

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“…( Dai et al, 2006;Jiang et al, 2007). The studies showed that there is a slight increase in serum AST and ALT activities at 1 day after infusion of plasmid DNA, followed by rapid decrease to the background level by day 3.…”

Section: Hsc-specific Hgf Delivery Retards Liver Fibrosis 517mentioning

confidence: 99%

“…The protocol for retrograde intrabiliary infusion was adapted from our previous work ( Jiang et al, 2007). Rats were anaesthetized with ketamine/xylazine, the common bile duct was canulated with a 32G needle (Hamilton), and a tie with 5-O silk sutures was placed to secure the needle.…”

Section: Retrograde Intrabiliary Infusionmentioning

confidence: 99%

Hepatic Stellate Cell–Targeted Delivery of Hepatocyte Growth Factor Transgene via Bile Duct Infusion Enhances Its Expression at Fibrotic Foci to Regress Dimethylnitrosamine-Induced Liver Fibrosis

et al. 2013

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Liver fibrosis generates fibrotic foci with abundant activated hepatic stellate cells and excessive collagen deposition juxtaposed with healthy regions. Targeted delivery of antifibrotic therapeutics to hepatic stellate cells (HSCs) might improve treatment outcomes and reduce adverse effects on healthy tissue. We delivered the hepatocyte growth factor (HGF) gene specifically to activated hepatic stellate cells in fibrotic liver using vitamin A-coupled liposomes by retrograde intrabiliary infusion to bypass capillarized hepatic sinusoids. The antifibrotic effects of DsRed2-HGF vector encapsulated within vitamin A-coupled liposomes were validated by decreases in fibrotic markers in vitro. Fibrotic cultures transfected with the targeted transgene showed a significant decrease in fibrotic markers such as transforming growth factor-b1. In rats, dimethylnitrosamineinduced liver fibrosis is manifested by an increase in collagen deposition and severe defenestration of sinusoidal endothelial cells. The HSC-targeted transgene, administered via retrograde intrabiliary infusion in fibrotic rats, successfully reduced liver fibrosis markers alpha-smooth muscle actin and collagen, accompanied by an increase in the expression of DsRed2-HGF near the fibrotic foci. Thus, targeted delivery of HGF gene to hepatic stellate cells increased the transgene expression at the fibrotic foci and strongly enhanced its antifibrotic effects.

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PEG-b-PPA/DNA micelles improve transgene expression in rat liver through intrabiliary infusion

Cited by 41 publications

References 36 publications

Mannosylated self-assembled structures for molecular confinement and gene delivery applications

Mannosylated self-assembled structures for molecular confinement and gene delivery applications

Energy Transfer with Semiconductor Quantum Dot Bioconjugates: A Versatile Platform for Biosensing, Energy Harvesting, and Other Developing Applications

Hepatic Stellate Cell–Targeted Delivery of Hepatocyte Growth Factor Transgene via Bile Duct Infusion Enhances Its Expression at Fibrotic Foci to Regress Dimethylnitrosamine-Induced Liver Fibrosis

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