PEG-based hydrogels as an in vitro encapsulation platform for testing controlled β-cell microenvironments

Weber, Laney M.; He, Jing; Bradley, Brenda J.; Haskins, Kathryn; Anseth, Kristi S.

doi:10.1016/j.actbio.2005.10.005

Cited by 127 publications

(134 citation statements)

References 25 publications

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“…Initially, methacrylate-functionalized PEG macromers were used due to their ease of synthesis and successful use with myriad cell types. [32][33][34]37,38,40,71 To screen SMG cytocompatibility, nongelling polymerizations were used to alleviate potential issues with diffusional constraints 28,59 by using macromers with only one functionality (Fig. 1A).…”

Section: Resultsmentioning

confidence: 99%

“…These data support the idea that cell-cell interactions are critical for viability of primary SMG cells, and are in agreement with a previous report showing that cell viability is lost when microspheres are dissociated. 10 Preserving cell-cell interactions has been found to promote the viability of other cell types such as pancreatic b-cells and motor neurons when encapsulated within PEG hydrogels, 38,41 supporting the rationale for the assembly of these multicellular ''microtissues'' before encapsulation. reports.…”

Section: Figmentioning

confidence: 99%

“…PEG hydrogels are bioinert, 26 and they lack cell-matrix and cell-cell interactions that are commonly utilized to maintain survivability of sensitive cell types. 32,38,41,49,50 As cell-cell interactions, in particular, play a vital role in salivary gland cell functions in vitro and during gland development, 20,[51][52][53][54][55][56][57] we also explored the use of SMG cell aggregation into microspheres to increase longterm viability of hydrogel-encapsulated SMG cells. Finally, we examined the cellular composition and proliferative potential of the encapsulated SMG microspheres.…”

mentioning

confidence: 99%

“…27 PEG hydrogels are traditionally formed through radicalmediated photopolymerizations, 28,29 which allow for rapid fabrication into geometries dictated by custom molds in vitro or to match tissue defects in vivo. 30,31 PEG hydrogels have been utilized successfully to culture and control the behavior of various cell types, including MSCs, 32,33 chondrocytes, [34][35][36] osteoblasts, 37 pancreatic b-islet cells, 38,39 and neurons. 40,41 In addition, recent work has demonstrated the ability of PEG hydrogels to provide spatiotemporal control of MSC delivery in vivo to promote bone regeneration.…”

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confidence: 99%

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Development of Poly(Ethylene Glycol) Hydrogels for Salivary Gland Tissue Engineering Applications

Shubin

Felong

Graunke

et al. 2015

Tissue Engineering Part A

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More than 40,000 patients are diagnosed with head and neck cancers annually in the United States with the vast majority receiving radiation therapy. Salivary glands are irreparably damaged by radiation therapy resulting in xerostomia, which severely affects patient quality of life. Cell-based therapies have shown some promise in mouse models of radiation-induced xerostomia, but they suffer from insufficient and inconsistent gland regeneration and accompanying secretory function. To aid in the development of regenerative therapies, poly(ethylene glycol) hydrogels were investigated for the encapsulation of primary submandibular gland (SMG) cells for tissue engineering applications. Different methods of hydrogel formation and cell preparation were examined to identify cytocompatible encapsulation conditions for SMG cells. Cell viability was much higher after thiol-ene polymerizations compared with conventional methacrylate polymerizations due to reduced membrane peroxidation and intracellular reactive oxygen species formation. In addition, the formation of multicellular microspheres before encapsulation maximized cell-cell contacts and increased viability of SMG cells over 14-day culture periods. Thiol-ene hydrogel-encapsulated microspheres also promoted SMG proliferation. Lineage tracing was employed to determine the cellular composition of hydrogel-encapsulated microspheres using markers for acinar (Mist1) and duct (Keratin5) cells. Our findings indicate that both acinar and duct cell phenotypes are present throughout the 14 day culture period. However, the acinar:duct cell ratios are reduced over time, likely due to duct cell proliferation. Altogether, permissive encapsulation methods for primary SMG cells have been identified that promote cell viability, proliferation, and maintenance of differentiated salivary gland cell phenotypes, which allows for translation of this approach for salivary gland tissue engineering applications.

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Section: Resultsmentioning

confidence: 99%

Section: Figmentioning

confidence: 99%

mentioning

confidence: 99%

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confidence: 99%

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Development of Poly(Ethylene Glycol) Hydrogels for Salivary Gland Tissue Engineering Applications

Shubin

Felong

Graunke

et al. 2015

Tissue Engineering Part A

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“…Various approaches have been employed to immobilize these thin films on solid surfaces. These techniques include electron beam irradiation, 27,28 photoinitiated grafting, 8,29,30 use of activated and functionalized substrates, 31,32 and plasma polymerization. 13,14,[33][34][35] The prior plasma generated films, noted above, involved the use of NIPAM monomer.…”

Section: Introductionmentioning

confidence: 99%

A New Class of Thin Film Hydrogels Produced by Plasma Polymerization

et al. 2007

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A simple, direct route to preparation of surface immobilized hydrogel films is described. Specifically, low pressure RF pulsed plasma polymerization of 1-amino-2-propanol and 2-(ethylamino)ethanol monomers produced thin hydrogel films deposited on substrates located in the plasma reactor. The successful syntheses were carried out under plasma conditions which not only yield the hydrogel but are also sufficiently energetic to produce films strongly grafted to the substrates. The polymer films obtained exhibit the thermoresponsive property of hydrogels, as shown by film color change with temperature. Additional evidence for the phase transition properties of these films was obtained using water contact angle and capillary rise measurements. The plasma polymerization approach provides an unusually simple route to synthesis of hydrogels in which the films are pin-hole free and are of easily controlled thickness. An important added advantage, particularly for applications involving biomaterials, is the conformal property of the plasma generated polymer films. The results obtained suggest that this approach should be applicable to a variety of other monomers and, based on differences observed with the present two monomers, suggest synthesis of films which exhibit a range of phase transition temperatures.

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Therapy with Cell Encapsulation for Substitution of Organ Function and Tumor Treatment

et al. 2009

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Cell encapsulation represents an innovative technique. However, clinical applications are sparse. Most experiments and clinical studies have been performed with either alginate or cellulose sulfate capsules, containing several cell lines and a broad variety of applications, ranging all the way from substitution for impaired organ function and release of cytokines or growth factors to gene‐directed enzyme prodrug therapy. A few clinical studies have been conducted and/or are under way.

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PEG-based hydrogels as an in vitro encapsulation platform for testing controlled β-cell microenvironments

Cited by 127 publications

References 25 publications

Development of Poly(Ethylene Glycol) Hydrogels for Salivary Gland Tissue Engineering Applications

Development of Poly(Ethylene Glycol) Hydrogels for Salivary Gland Tissue Engineering Applications

A New Class of Thin Film Hydrogels Produced by Plasma Polymerization

Therapy with Cell Encapsulation for Substitution of Organ Function and Tumor Treatment

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