PEI-assisted boronate affinity magnetic nanoparticle-based SELEX for efficient <i>in vitro</i> evolution of saponin-binding aptamers

Zhang, Hui; Li, Xue; Huang, Ailan; Zhang, Yan; Chen, Yang; Bie, Zijun

doi:10.1039/d1ra00889g

Cited by 3 publications

(2 citation statements)

References 41 publications

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“…The magnetic property of MNPs enables the simple and facile partitioning of oligos following the incubation of the target with the oligos. Recently, MNPs have been combined with a molecularly-imprinted polymer (MIP) to endow functionality to the SELEX platform [ 76 , 77 , 78 , 79 ]. MIPs are artificial molecules that specifically bind to template molecules.…”

Section: Current Aptamer Discovery Strategies To Detect Chemicals and Biomoleculesmentioning

confidence: 99%

“…This pH-induced binding/dissociation reaction ensures simple and efficient recovery of target-bound aptamers from MNPs. Binding abilities and affinities for the target could be improved further by functionalizing MNPs with polyethyleneimine, which forms a branched platform to bind more boronic acid moieties on the MNPs [ 79 ]. This platform could screen aptamers for two saponins (ginsenoside Re and Rb1) with K d values of 2.3 ± 0.3 (for Re), 4.9 ± 0.3 μM (for Rb1).…”

Section: Current Aptamer Discovery Strategies To Detect Chemicals and Biomoleculesmentioning

confidence: 99%

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Recent Advances in Micro/Nanomaterial-Based Aptamer Selection Strategies

Kim

Lee

et al. 2021

Molecules

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Aptamers are artificial nucleic acid ligands that have been employed in various fundamental studies and applications, such as biological analyses, disease diagnostics, targeted therapeutics, and environmental pollutant detection. This review focuses on the recent advances in aptamer discovery strategies that have been used to detect various chemicals and biomolecules. Recent examples of the strategies discussed here are based on the classification of these micro/nanomaterial-mediated systematic evolution of ligands by exponential enrichment (SELEX) platforms into three categories: bead-mediated, carbon-based nanomaterial-mediated, and other nanoparticle-mediated strategies. In addition to describing the advantages and limitations of the aforementioned strategies, this review discusses potential strategies to develop high-performance aptamers.

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