Peningkatan kualitas planlet tanaman pisang raja bulu (Musa paradisiaca) dengan penambahan bap dan iaa pada media pengakaran kultur in vitro

Nofiyanto, Rizal Try; Kusmiyati, Florentina; Karno, Karno

doi:10.14710/joac.3.3.132-141

Cited by 4 publications

(4 citation statements)

References 5 publications

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“…The treatment of BAP 5 mg L -1 + IAA 0.1 mg L -1 and BAP 5 mg L -1 + IAA 0.5 mg L -1 showed average shoot length 1.25-1.51 cm at 5 th and 7 th WAI (Table 5). A similar study was shown [10] that the treatment of 22 M BAP (4.96 mg L -1 ) + 2 M IAA (0.35 mg L -1 ) resulted in the longest average shoot length. The shoot elongation of the Barangan Merah cultivar explants was better in the combination treatment of BAP and IAA compared to the giving of single BAP.…”

Section: The Average Shoot Length Per Explantsupporting

confidence: 70%

“…The combination treatment of BAP and IAA on different cultivars resulted in different growth responses. This was shown in combination of BAP 1 mg L -1 and IAA 3mg L -1 resulted the best explant growth for Raja Bulu cultivars [10].…”

Section: The Average Shoot Length Per Explantmentioning

confidence: 75%

“…Multiplication of Kepok Merah banana shoots on MS medium with IAA 0.5 mg L -1 and BA 5 mg L -1 gave better results in shoot elongation and shoot propagation (6-17 shoots per explant) [9]. The concentration of 1 mg L -1 BAP and 3 mg L -1 IAA resulted in the best explant growth in Raja Bulu cultivars [10]. Therefore, in this study, our main objective is to determine the effective concentration of BAP and IAA for optimal multiple bud clumps induction in explants of Barangan Merah banana.…”

Section: Introductionmentioning

confidence: 93%

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Multiple bud clumps induction of banana cv. Barangan Merah (Musa acuminate Colla.) under in vitro treatment of benzyl amino purine and indole-3 acetic acid

Setyowati

Efendi

Basyah

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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Cultivar Barangan is one of the famous banana plants that has good taste and price. The disadvantages of this banana is susceptible to Fusarium disease. Tissue culture techniques is one ways of plants propagation for this plant. This study is aimed to determine the effective concentration of benzyl amino purines (BAP) and indole-3 acetic acid (IAA) for optimal multiple bud clumps (MBC) induction in explants of Barangan Merah banana. The Research was carried out at the Laboratory of Tissue Culture, Faculty of Agriculture, Universitas Syiah Kuala. The research design was a completely randomized design, 3x2 factorial. BAP concentration was the first factor, i.e. 5, 10 and 15 mg L−1. The second factor was the concentration of IAA, i.e. 0.1 and 0.5 mg L−1. The results of this research showed that 96,29% explants of all treatments survived. Explant contamination was 11,11% occurred by bacteria and brownish exudate that spread to the medium. MBC are significantly increased at 16 days after induction at concentration of BAP 5 mg L−1 + IAA 0.5 mg L−1. All combinations of BAP and IAA concentrations have produced MBC explants (100%) at eighth weeks after induction. The concentration of 5 mg L−1 BAP + 0.5 mg L−1 IAA resulted the highest shoots number. Analysis of variance and Least Significant Different further test showed that the longest of shoot length was founding the concentration of 5 mg L−1 BAP compared to other treatments. Increasing concentrations of BAP 10 mg L−1and 15 mg L−1 resulted in significantly lower shoot length. It can be concluded that taken together the concentration of 5 mg L−1 BAP + 0.5 mg L−1 IAA is the best to effectively multiple bud clumps induction in explants of Barangan Merah banana through tissue culture technique.

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Section: The Average Shoot Length Per Explantsupporting

confidence: 70%

Section: The Average Shoot Length Per Explantmentioning

confidence: 75%

Section: Introductionmentioning

confidence: 93%

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Multiple bud clumps induction of banana cv. Barangan Merah (Musa acuminate Colla.) under in vitro treatment of benzyl amino purine and indole-3 acetic acid

Setyowati

Efendi

Basyah

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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“…The plant that can be used as a source of explant is derived from the leaves, roots, bud eyes, pollen, stem ends, and bulbs maintained sterile. Factors that influence success in tissue culture are genotypes, culture media, growing environments, and explants (Nofiyanto et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

The combination of murashige and skoog (MS) media and activated charcoal on the growth of the Vanda helvola orchid plant in vitro

Wasiati

Nugraheni

Setiawati³

2021

Int. Journal Health Scie. Tech.

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Orchids of the Vanda helvola genus are extensively grown as ornamental plants. The method of propagation of plant seeds in tissue culture is an alternative that may be employed in the provision of a short period. Murashige and Skoog (MS) media and activated charcoal can optimize orchid cultivation utilizing tissue culture techniques. One of the keys to success in tissue culture is the application of natural types of growing regulatory substances (ZPT) at the proper concentration, which can lower the cost of orchid tissue culture.The objective of this study was to see how a combination of Murashige and Skoog (MS) media and activated charcoal influenced the Vanda helvola orchid subculture in vitro. The researchers employed a Complete Randomized Design (RAL) with four treatments and three repeats: negative control (K), ¼ MS (K1), ½ MS (K2), and 100% MS (K3) (K3). The observed parameters include the percentage of live explant, number of roots, and height of orchid plants. The results showed that the explant had a 100% life percentage of over 21 HST and had no noticeable influence on the number of roots and the plant's height.

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The effect of indole-3-acetic-acid and benzyl amino purine concentrations on matoa (Pometia pinnata) growth in vitro

Yuniastuti

Pratami

Sukaya

et al. 2022

IOP Conf. Ser.: Earth Environ. Sci.

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Matoa, a Papuan fruit, is known to taste like a cross between longan, rambutan, and durian. Therefore, Matoa has seed storage and plant propagation restrictions. The tissue culture method of matoa propagation is used to solve this issue. This research aims to provide vegetative planting material that can be propagated in vitro. The study was conducted from June 2021 to January 2022 at the Laboratory of Plant Physiology and Biotechnology, Faculty of Agriculture, Sebelas Maret University, Surakarta, using a completely randomized design pattern (CRD) 2x4 treatment factors for growth regulators. There were 16 treatment combinations, which were repeated three times. The first factor is Indole Acetic Acid (IAA) concentration, which has four levels: 0 ppm, 0.5 ppm, 1 ppm, and 1.5 ppm. The second factor is Benzyl Amino Purine (BAP) concentration, which has four levels: 0 ppm, 2 ppm, 4 ppm, and 6 ppm. The results revealed that root emergence time, leaf emergence time, root number, and leaf number all had high growth rates. The number of leaves and stem nodes correlated, indicating that matoa explant growth was linked.

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Peningkatan kualitas planlet tanaman pisang raja bulu (Musa paradisiaca) dengan penambahan bap dan iaa pada media pengakaran kultur in vitro

Cited by 4 publications

References 5 publications

Multiple bud clumps induction of banana cv. Barangan Merah (Musa acuminate Colla.) under in vitro treatment of benzyl amino purine and indole-3 acetic acid

Multiple bud clumps induction of banana cv. Barangan Merah (Musa acuminate Colla.) under in vitro treatment of benzyl amino purine and indole-3 acetic acid

The combination of murashige and skoog (MS) media and activated charcoal on the growth of the Vanda helvola orchid plant in vitro

The effect of indole-3-acetic-acid and benzyl amino purine concentrations on matoa (Pometia pinnata) growth in vitro

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