Pentadecanoic Acid, an Odd-Chain Fatty Acid, Suppresses the Stemness of MCF-7/SC Human Breast Cancer Stem-Like Cells through JAK2/STAT3 Signaling

To, Ngoc Bao; Nguyen, Yen Thi-Kim; Moon, Jeong Yong; Ediriweera, Meran Keshawa; Cho, Somi Kim

doi:10.3390/nu12061663

Cited by 75 publications

(63 citation statements)

References 99 publications

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“…In GC-MS analysis, most of the identified constituents have been reported to possess important biological activities. For example, linoleic acid, palmitic acid, linolenic acid, pentadecanoic acid, oleic acid and stearic acid have cytotoxic activity against various cancer cell lines [18][19][20][21][22]. On the basis of the available information; it is assumed that the anti-breast cancer effect of n-hexane fraction may be due to the presence of fatty acid methyl esters.…”

Section: Discussionmentioning

confidence: 99%

Chemical profiling and anti-breast cancer potential of hexane fraction of Sphaeranthus indicus flowers

Rasheed¹,

Wahid²,

Ikram³

et al. 2021

Trop. J. Pharm Res

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Purpose: The current study aimed to determine the phytochemicals and anti-breast cancer potential of Sphaeranthus indicus.Methods: S. indicus flowers were extracted with methanol followed by fractionation using n-hexane. For the chemical composition of n-hexane fraction, qualitative phytochemical and GC-MS analysis were performed. The anti-proliferative activity was measured by MTT assay, whereas, cytotoxic and proapoptotic effects in MCF-7 (breast cancer) cells were determined using propidium iodide, 4′,6-diamidino- 2-phenylindole, dichlorofluorescin diacetate, and JC-1 staining through fluorescent microscopy.Results: The phytochemical analysis indicated presence of phytosterols, oils and resins in the nhexane fraction. GC-MS analysis showed that n-hexane fraction comprises of 11 compounds including methyl esters of caprylic acid, myristic acid, pentadecanoic acid, palmitic acid, margaric acid, stearic acid, oleic acid, elaidic acid, linoleic acid, linolenic acid and behanic acid. The tested fraction showed remarkable cytotoxic activity against breast cancer (MCF-7) cells while it was found less toxic towards non-cancerous (BHK-21) cells. Furthermore, morphological assessment through fluorescent microscopy revealed cytotoxic and apoptotic effects by improved cell membrane permeability, increased reactive oxygen species level, compromised mitochondrial activity and condensation of chromatin network.Conclusion: The n-hexane fraction of S. indicus contains phytosterols, oils and fatty acid methyl esters and produced apoptotic effect against breast cancer cells.

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Section: Discussionmentioning

confidence: 99%

Chemical profiling and anti-breast cancer potential of hexane fraction of Sphaeranthus indicus flowers

Rasheed¹,

Wahid²,

Ikram³

et al. 2021

Trop. J. Pharm Res

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“…Methoxyeugenol has anticancer activity and antimicrobial activity (14) . Pentadecanoic acid, suppresses the stemness of mcf-7/sc human breast cancer stem-like cells through JAK2/STAT3 signaling (15 ) . Dodecanoic acid, may elicit apoptosis in certain cancer cells (16) and exerted antiproliferative activity in diverse types of tumor cells (17) .…”

Section: Resultsmentioning

confidence: 99%

GC-Mass Spectrometry Analysis of Iraqi Moringa Oleifera Seeds Extract

AL-Obaidi¹,

Thaker²,

Ramizy³

2021

IJFMT

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Moringa oleifera is an important traditional medicinal plant usedfor the treatment of many health problems. It is widely cultivated across the world but it has not well known orwell-studied in Iraq. Therefore, the aim of this study is to identify the bioactive compounds present in acetone:methanol (1:1) seeds extractof Iraqi Moringa olieifera by using GC-Mass Spectrophotometry.The results of this analysis revealed 41 compounds which include ,terpenes ( cis-beta-Ocimene 0.

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“…The MTT cell viability assay was conducted as previous described [ 60 ]. Briefly, cells (2 × 10 4 cells/mL) were seeded into 96-well plates exposed to different doses of ampelopsin (TCI, Tokyo, Japan).…”

Section: Methodsmentioning

confidence: 99%

“…The cell invasion assay was conducted on 24-well Transwell plates (Corning, Cambridge, MA, USA) as described previously [ 60 ]. The upper chambers were coated with 1% Matrigel.…”

Section: Methodsmentioning

confidence: 99%

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Ampelopsin Suppresses Stem Cell Properties Accompanied by Attenuation of Oxidative Phosphorylation in Chemo- and Radio-Resistant MDA-MB-231 Breast Cancer Cells

2021

Self Cite

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Ampelopsin, also known as dihydromyricetin, is a commonly found flavonoid in medicinal plants. The cancer stem cell (CSC) population is a promising target for triple-negative breast cancer (TNBC). In this study, flavonoid screening was performed in the established MDA-MB-231/IR cell line, which is enriched in CSCs. Ampelopsin suppressed the proliferation and colony formation of stem cell-rich MDA-MB-231/IR, while inducing their apoptosis. Importantly, ampelopsin displayed an inhibitory impact on the stemness features of MDA-MB-231/IR cells, demonstrated by decreases in mammosphere formation, the CD44+/CD24−/low population, aldehyde dehydrogenase activity, and the levels of stem cell markers (e.g., CD44, MRP1, β-catenin, and KLF4). Ampelopsin also suppressed the epithelial–mesenchymal transition, as evidenced by decreases in migration, invasion capacity, and mesenchymal markers, as well as an increase in the epithelial marker E-cadherin. Moreover, ampelopsin significantly impaired oxidative phosphorylation by reducing the oxygen consumption rate and adenosine triphosphate production in MDA-MB-231/IR cells. Notably, ampelopsin treatment significantly reduced the levels of the phosphorylated forms of IκBα and NF-κB p65, as well as the levels of tumor necrosis factor (TNF)-α-stimulated phosphorylation of IκBα and NF-κB p65. These results demonstrated that ampelopsin prevents the TNF-α/NF-κB signaling axis in breast CSCs.

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Pentadecanoic Acid, an Odd-Chain Fatty Acid, Suppresses the Stemness of MCF-7/SC Human Breast Cancer Stem-Like Cells through JAK2/STAT3 Signaling

Cited by 75 publications

References 99 publications

Chemical profiling and anti-breast cancer potential of hexane fraction of Sphaeranthus indicus flowers

Chemical profiling and anti-breast cancer potential of hexane fraction of Sphaeranthus indicus flowers

GC-Mass Spectrometry Analysis of Iraqi Moringa Oleifera Seeds Extract

Ampelopsin Suppresses Stem Cell Properties Accompanied by Attenuation of Oxidative Phosphorylation in Chemo- and Radio-Resistant MDA-MB-231 Breast Cancer Cells

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