Pentagalloylglucose down‐regulates mast cell surface FcεRI expression in vitro and in vivo

Abstract

a b s t r a c tMast cell activation by immunoglobulin E (IgE)-mediated stimuli is a central event in the pathogenesis of allergic disorders. The present report shows that treatment with pentagalloylglucose (PGG) resulted in a down-regulation of FceRI surface expression on mucosal-type murine bone marrowderived mast cells (mBMMCs), which correlated with a reduction in IgE-mediated activation of mBMMCs. Furthermore, PGG prevented development of allergic diarrhea in a food-allergy mouse model and suppressed the up-regulated FceRI surface expression on mast cells derived from the food-allergy mouse colon. These findings on PGG suggest its therapeutic potential for allergic diseases through suppressing the FceRI surface expression.

Shikonin, a constituent of Lithospermum erythrorhizon exhibits anti-allergic effects by suppressing orphan nuclear receptor Nr4a family gene expression as a new prototype of calcineurin inhibitors in mast cells

“…Previously, we have demonstrated that mucosal mast cells are primarily responsible for the pathology of FA in a murine FA model [6]. Connective tissue mast cell stabilizers (tranilast, ketotifen and cromolyn) and anti-histamine agents are frequently used to treat various allergic disorders other than FA, but these agents have no effect on mucosal mast cells [7]. Until now, little has been known about inhibitory agents against mucosal mast cell activation.…”

Anti-Allergic Drugs Tranilast and Ketotifen Dose-Dependently Exert Mast Cell-Stabilizing Properties

“…However, later studies using human conjunctival mast cells additionally revealed a mast cell-stabilizing property of ketotifen [7,8], consistent with its higher potency in the treatment of allergic disorders. So far, by measuring the amount of chemokines released from rat peritoneal mast cells, such as histamine and β-hexosaminidase, previous studies have suggested the superiority of ketotifen to tranilast in the properties of mast cell stabilization [9][10][11]. However, due to the limitations of the methods used in molecular biology [12], the effects of these drugs on the exocytotic process of mast cells have not directly been examined.…”

Rab5a Regulates Surface Expression of Fc.EPSILON.RI and Functional Activation in Mast Cells

“…14) FceRI, like a large number of cell surface receptors, is assumed to be dynamically transported within the cell. Rab5 has been shown to be a key regulator of various receptors transport in the early endocytic pathway.…”