Peptide aptamer based on natural protein ligand for C reactive protein detection

Abstract

As a substitute for traditional antibodies, peptide aptamers have received extensive attention in recent years. However, the current research can only screen peptide aptamers from antibody sequence structures. This study changed the inherent idea that peptide aptamers were derived from antibodies in the past. Screening of peptide aptamers derived from natural protein ligands ( complement C1q ) of C-reactive proteinby bioinformatics technology to achieve specific detection of CRP, and establishing a CRP detection method in which peptide aptamers replace traditional antibodies to play a capture role. It is proved that peptide aptamers derived from natural protein ligands are feasible for detecting antigens. The results showed that the peptide aptamer C199 − 207m screened by bioinformatics could specifically bind to CRP, and the established sandwich ELISA technology with capture function of peptide aptamer C199 − 207m could be used to detect CRP with good accuracy, specificity, repeatability, sensitivity and ideal detection range. Peptide aptamers based on natural protein ligands replace traditional antibodies to capture antigens, which is a new attempt that can be applied to the immunoassay of CRP or related proteins.