Peptide Aptamers That Bind to Geminivirus Replication Proteins Confer a Resistance Phenotype to
            <i>Tomato Yellow Leaf Curl Virus</i>
            and
            <i>Tomato Mottle Virus</i>
            Infection in Tomato

Reyes, Maria Ines; Nash, Tara; Dallas, Mary M.; Ascencio-Ibáñez, José Trinidad; Hanley‐Bowdoin, Linda

doi:10.1128/jvi.01095-13

Cited by 70 publications

(79 citation statements)

References 87 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In Nigeria, moderate resistance in four pepper cultivars has been identified [79]. In addition to the conventional breeding, different transgenic approaches for achieving resistance or tolerance have been tested against begomoviruses infecting tomato and pepper [80][81][82] and this may be a way forward for obtaining durable resistance. In West and Central Africa, leaf curl disease is regarded as the most important biotic stress for okra, and there is a lack of viral resistance/tolerance [58,83].…”

Section: Resistancementioning

confidence: 99%

Begomovirus disease complex: emerging threat to vegetable production systems of West and Central Africa

et al. 2015

View full text Add to dashboard Cite

Vegetables play a major role in the livelihoods of the rural poor in Africa. Among major constraints to vegetable production worldwide are diseases caused by a group of viruses belonging to the genus Begomovirus, family Geminiviridae. Begomoviruses are plant-infecting viruses, which are transmitted by the whitefly vector Bemisia tabaci and have been known to cause extreme yield reduction in a number of economically important vegetables around the world. Several begomoviruses have been detected infecting vegetable crops in West and Central Africa (WCA). Small single stranded circular molecules, alphasatellites and betasatellites, which are about half the size of their helper begomovirus genome, have also been detected in plants infected by begomoviruses. In WCA, B. tabaci has been associated with suspected begomovirus infections in many vegetable crops and weed species. Sequencing of viral genomes from crops such as okra resulted in the identification of two previously known begomovirus species (Cotton leaf curl Gezira virus and Okra yellow crinkle virus) as well as a new recombinant begomovirus species (Okra leaf curl Cameroon virus), a betasatellite (Cotton leaf curl Gezira betasatellite) and new alphasatellites. Tomato and pepper plants with leaf curling were shown to contain isolates of new begomoviruses, collectively referred to as West African tomato-infecting begomoviruses (WATIBs), new alphasatellites and betasatellites. To study the potential of weeds serving as begomovirus reservoirs, begomoviruses and satellites in the weed Ageratum conyzoides were characterized. Sequence analyses showed that they were infected by isolates of a new begomovirus (Ageratum leaf curl Cameroon virus) that belong to the WATIBs group, a new betasatellite (Ageratum leaf curl Cameroon betasatellite), an alphasatellite and two types of defective recombinants between a begomovirus and an alphasatellite. Putative recombinations were detected in begomovirus genomes for all four plant species studied, indicating that recombination is an important mechanism for their evolution. A close relationship between the begomoviruses infecting pepper and tomato and A. conyzoides and the detection of the same alphasatellite in them support the idea that weeds are important reservoirs for begomoviruses and their satellites. With this high diversity, recombination potential and transmission by B. tabaci, begomoviruses and ssDNA satellites pose a serious threat to crop production in West and Central Africa.

show abstract

Section: Resistancementioning

confidence: 99%

Begomovirus disease complex: emerging threat to vegetable production systems of West and Central Africa

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Similar study was also performed in TYLCV and Tomato mottle virus infection, in which peptide aptamers interacting with Rep proteins were targeted. This study also revealed that two of the peptide aptamers interacted with the Rep proteins from viruses representing three major Geminiviridae genera, providing broad spectrum resistance [26].…”

Section: Peptide Aptamers Mediated Resistance Against Geminivirusesmentioning

confidence: 96%

Application of molecular antiviral compounds: novel approach for durable resistance against geminiviruses

Sahu

Prasad

2015

Mol Biol Rep

View full text Add to dashboard Cite

Both transgenic as well as traditional breeding approaches have not been completely successful in inducting resistance against geminiviruses in crop plants. This demands the utilization of non-viral, non-plant compounds possessing antiviral characteristics as an alternate and effective strategy for developing durable resistance against geminiviruses. In recent years, several antiviral molecules have been developed for the treatment of plant virus infections. These molecular antiviral compounds target various geminiviral-DNA and -protein via interacting with them or by cleaving viral RNA fragments. Applications of these proteins such as GroEL, g5g and VirE2 have also provided a convincing evidence of resistance against geminiviruses. Taking advantage of this information, we can generate robust resistance against geminiviruses in diverse crop plants. In this context, the present review provides epigrammatic information on these antiviral compounds and their mode of action in modulating virus infection.

show abstract

“…Peptide aptamers are small peptides, usually no longer than 20 amino acids, that interfere with protein interactions. Transgenic tomato lines expressing aptamers selected from a library of random sequences by repeated screening were shown to exhibit no or very mild symptoms upon infection with either TYLCV or ToMoV and to contain lower levels of viral DNA (Reyes et al, 2013).…”

Section: Other Control Strategiesmentioning

confidence: 99%

Geminiviridae

Briddon

2015

Encyclopedia of Life Sciences

View full text Add to dashboard Cite

The Geminiviridae comprise a group of plant viruses with small, circular, single‐stranded deoxyribonucleic acid (ssDNA) genomes encapsidated in particles that have a twinned quasi‐icosahedral (geminate) shape, from which the family derives its name. They are among the smallest of viruses known and are transmitted exclusively by specific insect vectors. Some are associated with highly diverse circular, ssDNA satellites that play a part in disease progression and provide evidence of the interaction of geminiviruses with other families of plant‐infecting viruses. Collectively, geminiviruses are pathogens of cereals, vegetable and fibre crops, posing a serious threat to agriculture worldwide. Efforts to control these viruses by conventional means have either proven unsuccessful or have not been durable. For this reason, researchers are increasingly looking to engineered resistance as a means to control these important viruses. This has recently led to the first crop with engineered resistance to a geminivirus reaching the field. Key Concepts Geminiviruses are important insect‐transmitted pathogens of cereal, vegetable and fibre crops in tropical and temperate regions throughout the world, including developed and developing countries. Geminiviruses often cause striking symptoms in plants, indeed the earliest description of virus symptoms in plants is believed to relate to those caused by a geminivirus and such symptoms occasionally are considered to be horticulturally desirable. All geminiviruses have single‐stranded DNA genomes and a twinned particle morphology that is unique to this family of plant viruses. Some geminiviruses are associated with single‐stranded DNA satellites, referred to as alpha‐ and betasatellites, the latter playing an essential role in maintenance of the disease. Because their small DNA genomes encode only a limited number of genes, geminiviruses manipulate the metabolism of the infected cell to produce conditions suitable for their proliferation. Geminiviruses are proving invaluable in the investigation of fundamental biological processes such as DNA replication, cell‐cycle regulation, gene expression and pathogen resistance in plants. Geminiviruses are highly recombinogenic, and the derivation of a large number of geminivirus and satellite DNA sequences is providing important insights into their diversity, adaptability and evolution. Conventional control strategies, such as the use of chemicals to eliminate their insect vectors and breeding natural host plant resistance, have proven ineffective in reducing crop losses to geminivirus infection, leading to the development of promising alternative (genetically engineered) strategies. Sequences derived from geminiviruses are being used to develop systems for high‐level expression of valuable proteins in plants.

show abstract

Peptide Aptamers That Bind to Geminivirus Replication Proteins Confer a Resistance Phenotype to Tomato Yellow Leaf Curl Virus and Tomato Mottle Virus Infection in Tomato

Cited by 70 publications

References 87 publications

Begomovirus disease complex: emerging threat to vegetable production systems of West and Central Africa

Begomovirus disease complex: emerging threat to vegetable production systems of West and Central Africa

Application of molecular antiviral compounds: novel approach for durable resistance against geminiviruses

Geminiviridae

Contact Info

Product

Resources

About