Peptide barcodes in dogs with pulmonary hypertension secondary to mitral valve disease using MALDI-TOF MS and LC-MS/MS

Abstract

Background Pulmonary hypertension (PH) is an important complication in dogs with cardiorespiratory diseases. The most common underlying disease of PH has been described to be mitral valve disease (MVD), which is the most frequent acquired heart disease found in dogs. Doppler echocardiography is generally used in routine practice for identifying PH; however, there are several limitations to this method in practical use. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a technique that can characterize specific patterns of peptide mass called peptide barcodes from various samples. In addition, in combination with liquid chromatography tandem mass spectrometry (LC-MS/MS), potential peptide sequences associated with specific conditions could be identified. The present study aimed to use MALDI-TOF coupled with LC-MS/MS to characterize specific peptide barcodes and potential peptide candidates in serum samples from healthy dogs (normal control), dogs with MVD stage B (MVD B) (asymptomatic stage), MVD stage C (MVD C) (symptomatic stage), MVD stage B with PH (MVD B PH) and MVD stage C with PH (MVD C PH). Results Discrete clusters of the 5 sample groups were identified by 3D plot analysis. Peptide barcodes also revealed differences in peptide patterns among the 5 groups. Six amino acid sequences of peptide candidates at 1,225.60, 1,363.85, 1,688.71, 1789.52, 2020.21 and 2156.42 Da were identified as part of the proteins CLCN1, CLUL1, EDNRA, PTEN, SLC39A7, and CLN6, respectively. The network interactions between these discovered proteins and common cardiovascular drugs were also investigated. Conclusion The present study revealed distinct clusters and different peptide barcodes for the MVD B, MVD C, MVD B PH, MVD C PH and normal control groups using MALDI-TOF MS. These results demonstrate that MALDI-TOF MS has promise as a technique for diagnosing dogs affected by asymptomatic and symptomatic stages of MVD with and without PH. Additionally, with MALDI-TOF MS in combination with LC-MS/MS, potential peptide candidates related to diseases were also identified. Further studies are required to identify peptide barcodes in dogs with other diseases to create peptide barcode databases in veterinary medicine before using this method as a novel diagnostic tool in the future.