Peptide-based biosensors

Liu, Qingtao; Wang, Jinfeng; Boyd, Ben J.

doi:10.1016/j.talanta.2014.12.020

Cited by 257 publications

(109 citation statements)

References 138 publications

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“…A szakirodalomban számos összefoglaló közlemény tárgyalja ilyen típusú receptorok optokémiai szenzorokban történõ alkalmazását. [5][6][7][8][9] A szintetikus oligopeptid szenzorok ugyan drágábbak, mint a sejt alapúak, azonban rendkívül érzékennyé és szelektívvé tehetõk. Emellett az optimális mûködési körülmények tekintetében alkalmazásuk rendszerint jóval kevésbé korlátozott, mint akár a sejt, akár a fehérje alapú szenzoroké.…”

Section: Bevezetésunclassified

“…6,11 Így nem meglepõ, hogy a peptid típusú receptoron alapuló fémion érzékelõk is leginkább fluorofór csoporto(ka)t tartalmaznak jelképzõ elemként. [5][6][7]9,12 A fluoreszcenciás elven mûködõ fémion-szenzorokat a fémion-kötõhely és a fluorofór csoport(ok) egymáshoz viszonyított helyzete alapján három nagyobb csoportba sorolhatjuk. 13 Az ún.…”

Section: Bevezetésunclassified

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Metalloproteinek fémkötőhelyein alapuló oligopeptidek, mint potenciális toxikus fémion érzékelők

Szekeres¹,

Szunyogh²,

Galbács³

et al. 2017

MKF

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Section: Bevezetésunclassified

Metalloproteinek fémkötőhelyein alapuló oligopeptidek, mint potenciális toxikus fémion érzékelők

Szekeres¹,

Szunyogh²,

Galbács³

et al. 2017

MKF

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“…The choice of an Id-peptide was dictated by two main reasons: (i) Artificial peptides provide an opportunity to develop the desired molecular biosensor due to their desirable properties such as diversified structure and high affinity [50]. In addition, peptides with specific sequences can provide high affinity to particular ligands and be obtained by screening and optimization of artificial peptide libraries; (ii) the used idiotype peptide is a small peptide ligand able to be recognized with high affinity and specificity from the B-cell receptors present on the lymphoma B cells [51][52][53].…”

Section: Choice Of Biomolecular Probementioning

confidence: 99%

Bioengineered Surfaces for Real-Time Label-Free Detection of Cancer Cells

Martucci¹,

Migliaccio²,

ImmacolataRuggiero³

et al. 2016

Lab-on-a-Chip Fabrication and Application

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Biosensing technology is an advancing field that benefits from the properties of biological processes combined to functional materials. Recently, biosensors have emerged as essential tools in biomedical applications, offering advantages over conventional clinical techniques for diagnosis and therapy. Optical biosensors provide fast, selective, direct, and cost-effective analyses allowing label-free and real-time tests. They have also shown exceptional potential for integration in lab-on-a-chip (LOC) devices. The major challenge in the biosensor field is to achieve a fully operative LOC platform that can be used in any place at any time. The choice of an appropriate strategy to immobilize the biological element on the sensor surface becomes the key factor to obtain an applicable analytical tool. In this chapter, after a brief description of the main biofunctionalization procedures on silicon devices, two silicon-based chips that present an (i) IgG antibody or (ii) an Id-peptide as molecular probe, directed against the B-cell receptor of lymphoma cancer cells, will be presented. From a comparison in detecting cells, the Id-peptide device was able to detect lymphoma cells also at low cell concentrations (8.5 × 10 −3 cells/μm 2 ) and in the presence of a large amount of non-specific cells. This recognition strategy could represent a proof-of-concept for an innovative tool for the targeting of patient-specific neoplastic B cells during the minimal residual disease; in addition, it represents an encouraging starting point for the construction of a lab-on-a-chip system for the specific recognition of neoplastic cells in biological fluids enabling the follow-up of the changes of cancer cells number in patients, highly demanded for therapy monitoring applications.

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“…This allowed detection of these peptides in plasma samples at 50 ng/mL level. Several commercial immobilized metal affinity chromatography (IMAC) sorbents were evaluated for the analysis of two small peptide fragments (A␤ (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15) and A␤ (10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20)) of the A␤ protein by on-line IMAC-SPE-CE-UV method [86]. The performance of a nickel (Ni 2+ ) metal ion sorbent with nitrilotriacetic acid as a chelating agent was significantly better than two copper (Cu 2+ ) ion sorbents with iminodiacetic acid.…”

Section: Preconcentration and Preseparationmentioning

confidence: 99%

“…Acting as hormones, neurotransmitters, immunomodulators, coenzymes, enzyme substrates and inhibitors, receptor ligands, ionophores, antibiotics, and toxins, they participate in control and regulation of many vitally important processes in all living organisms. In addition, many drugs and prodrugs are derived from peptides or peptidomimetics and some peptides are components of biosensors . In the current era of proteomics, peptidomics, and metabolomics—major methodologies in the investigation of the molecular bases of biological processes and diseases, as well as in searching for new biomarkers and drug targets—the importance of peptides is even increasing.…”

Section: Introductionmentioning

confidence: 99%

Recent developments in capillary and microchip electroseparations of peptides (2013–middle 2015)

Kašička

2015

Electrophoresis

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The review brings a comprehensive survey of recent developments and applications of high performance capillary and microchip electroseparation methods (zone electrophoresis, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, and electrochromatography) to analysis, micropreparation, purification, and physicochemical and biochemical characterization of peptides in the years 2013, 2014, and ca. up to the middle of 2015. Advances in the investigation of electromigration properties of peptides, in the methodology of their analysis, including sample preseparation, preconcentration and derivatization, adsorption suppression and EOF control, as well as in detection of peptides, are described. New developments in particular CE and CEC modes are presented and several types of their applications to peptide analysis are reported: conventional qualitative and quantitative analysis, determination in complex (bio)matrices, monitoring of chemical and enzymatical reactions and physical changes, amino acid, sequence, and chiral analysis, and peptide mapping of proteins. Some micropreparative peptide separations are shown and capabilities of CE and CEC techniques to provide important physicochemical characteristics of peptides are demonstrated.

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Peptide-based biosensors

Cited by 257 publications

References 138 publications

Metalloproteinek fémkötőhelyein alapuló oligopeptidek, mint potenciális toxikus fémion érzékelők

Metalloproteinek fémkötőhelyein alapuló oligopeptidek, mint potenciális toxikus fémion érzékelők

Bioengineered Surfaces for Real-Time Label-Free Detection of Cancer Cells

Recent developments in capillary and microchip electroseparations of peptides (2013–middle 2015)

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