Peptide-based fluorescent biosensors

Abstract

The use of fluorescent techniques in biological research is widespread. Many of the techniques rely on the use of fluorescent genetically-encoded tags (namely GFP and its different variants), but small molecules and nanoparticle-based approaches are being increasingly used. Peptides, owing to their modular nature, synthetic accessibility and biomolecular recognition potential, offer unique possibilities for the development of efficient and selective fluorescent sensors. In this tutorial review we present several of the strategies that have been used to develop fluorescent-based peptide sensors and discuss selected applications to biological problems.

Metalloproteinek fémkötőhelyein alapuló oligopeptidek, mint potenciális toxikus fémion érzékelők

“…6,11 Így nem meglepõ, hogy a peptid típusú receptoron alapuló fémion érzékelõk is leginkább fluorofór csoporto(ka)t tartalmaznak jelképzõ elemként. [5][6][7]9,12 A fluoreszcenciás elven mûködõ fémion-szenzorokat a fémion-kötõhely és a fluorofór csoport(ok) egymáshoz viszonyított helyzete alapján három nagyobb csoportba sorolhatjuk. 13 Az ún.…”

Solution phase photophysics of 5,7-dimethoxy-2,3,4,9-tetrahydro-1H-carbazol-1-one: Analysing the lineaments of a new fluorosensor to probe different micro-environments

“…These organic frameworks are particularly beneficial for fluorescent labelling as they encompass a broad array of photophysical properties including molar absorptivity, quantum yield, Stokes shift, lifetime and ability to respond towards the immediate environment [1,2]. Literature review [3][4][5] reports a large number of such small molecule environment sensitive fluorophores but the quest for newer ones is still on as they are extremely essential in the study of environmental indicators, molecular biology, cellular staining and for other biochemical processes. In this communication, we are introducing a new fluorophore 5,7-dimethoxy-2,3,4,9-tetrahydro-1H-carbazol-1-one (KTHC-57) (Scheme 1).…”

N,N'-{1,3-Phenylenebis[methyleneoxy-2,1-phenylene(Z)methylylidene]}bis[1-(1-naphthyl)methanamine]

“…The synthesis and characterisation of new fluorescence chemosensors has evolved rapidly in the last few years [1][2][3][4][5]. A fluorescence chemosensor is a compound that exhibits changes in its fluorescence properties upon interaction with an analyte.…”