Peptide epitope-imprinted polymer microarrays for selective protein recognition. Application for SARS-CoV-2 RBD protein

Bognár, Zsófia; Supala, Eszter; Yarman, Aysu; Zhang, Xiaorong; Bier, Frank F.; Scheller, Frieder W.; Gyurcsányi, Róbert E.

doi:10.1039/d1sc04502d

Cited by 41 publications

(34 citation statements)

References 52 publications

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“…The MIPs were applied for the determination of SARS-CoV-2 proteins and by methods other than electrochemical. The SPR method employing the highly affine MIPs for the recognition of the RBD of SARS-CoV-2 S-protein was developed by Bognar et al [ 148 ]. The SPR sensor modified with MIP imprinted peptide epitope was obtained by electrochemical deposition of polyscopoletin film.…”

Section: Covid-19 Diagnosismentioning

confidence: 99%

Biosensors for the Determination of SARS-CoV-2 Virus and Diagnosis of COVID-19 Infection

Drobysh

Ramanavičienė

Viter

et al. 2022

IJMS

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Monitoring and tracking infection is required in order to reduce the spread of the coronavirus disease 2019 (COVID-19), induced by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). To achieve this goal, the development and deployment of quick, accurate, and sensitive diagnostic methods are necessary. The determination of the SARS-CoV-2 virus is performed by biosensing devices, which vary according to detection methods and the biomarkers which are inducing/providing an analytical signal. RNA hybridisation, antigen-antibody affinity interaction, and a variety of other biological reactions are commonly used to generate analytical signals that can be precisely detected using electrochemical, electrochemiluminescence, optical, and other methodologies and transducers. Electrochemical biosensors, in particular, correspond to the current trend of bioanalytical process acceleration and simplification. Immunosensors are based on the determination of antigen-antibody interaction, which on some occasions can be determined in a label-free mode with sufficient sensitivity.

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Section: Covid-19 Diagnosismentioning

confidence: 99%

Biosensors for the Determination of SARS-CoV-2 Virus and Diagnosis of COVID-19 Infection

Drobysh

Ramanavičienė

Viter

et al. 2022

IJMS

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“…Later, Scheller’s group developed a fully electrochemical approach (including template removal: anodic potential pulses) on gold surfaces [ 79 ]. Epitope imprinting was also exploited for virus sensing for various viruses, comprising recently SARS-CoV-2 [ 39 , 58 , 72 , 80 , 81 , 82 , 83 ]. It is important to note that in the epitope approach, the area of specific interaction of the protein with the MIP is restricted to the epitope cavities.…”

Section: Molecularly Imprinted Polymersmentioning

confidence: 99%

“…For this purpose, milder imprinting techniques such as soft lithography and electropolymerization have been exploited. Both techniques have been utilized both for proteins and viruses [ 20 , 37 , 55 , 56 , 82 , 106 , 107 , 108 , 109 , 110 , 111 , 112 ].…”

Section: Molecularly Imprinted Polymersmentioning

confidence: 99%

“…In another study, Bognar et al developed an MIP sensor, which applied the nonapeptide 485–493 of the RBD as the epitope template ( Figure 6 ) [ 82 ]. The peptide GFNCYFPLQ was microspotted on gold SPR chips before the deposition of polyscopoletin.…”

Section: Mip-based Biomimetic Sensors For Sars-cov-2 Detectionmentioning

confidence: 99%

“… Schematic representation of steps of virus imprinting procedure preparation and the SPR responses of the rebinding Reprinted with permission from [ 82 ]. 2022, RSC.…”

Section: Figurementioning

confidence: 99%

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Molecularly Imprinted Polymer-Based Sensors for SARS-CoV-2: Where Are We Now?

Yarman

Kurbanoğlu

2022

Biomimetics

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Since the first reported case of COVID-19 in 2019 in China and the official declaration from the World Health Organization in March 2021 as a pandemic, fast and accurate diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has played a major role worldwide. For this reason, various methods have been developed, comprising reverse transcriptase-polymerase chain reaction (RT-PCR), immunoassays, clustered regularly interspaced short palindromic repeats (CRISPR), reverse transcription loop-mediated isothermal amplification (RT-LAMP), and bio(mimetic)sensors. Among the developed methods, RT-PCR is so far the gold standard. Herein, we give an overview of the MIP-based sensors utilized since the beginning of the pandemic.

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Eine Strep‐Tag‐geprägte Polymer‐Plattform für die heterogene Bio(elektro)katalyse

Yarman,

Waffo,

Katz

et al. 2024

Angewandte Chemie

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Molecularly imprinted polymers (MIPs) are artificial receptors equipped with selective recognition sites for target molecules. One of the most promising‐strategies for protein MIPs relies on the exploitation of short surface‐exposed protein fragments, termed epitopes, as templates to imprint binding sites in a polymer scaffold for a desired protein. However, the lack of high‐resolution structural data of flexible surface‐exposed regions challenges the selection of suitable epitopes. Here, we addressed this drawback by developing a polyscopoletin‐based MIP that recognizes recombinant proteins via the widely used Strep‐tag II affinity peptide. Electrochemistry, surface‐sensitive spectroscopy, and molecular dynamics simulations were employed to ensure an utmost control of the Strep‐MIP electrosynthesis. The functionality of this novel platform was verified with two Strep‐tag labeled enzymes: an O2‐tolerant [NiFe]‐hydrogenase, and an alkaline phosphatase. The enzymes preserved their biocatalytic activities after multiple utilization confirming the efficiency of Strep‐MIP as a general biocompatible platform to confine recombinant proteins for exploitation in biotechnology.

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Peptide epitope-imprinted polymer microarrays for selective protein recognition. Application for SARS-CoV-2 RBD protein

Abstract: We introduce highly affine epitope-imprinted polymer-based microarrays for selective protein detection by surface plasmon resonance imaging as shown through the selective recognition of the receptor binding domain of SARS-CoV-2 spike protein.

Cited by 41 publications

References 52 publications

Biosensors for the Determination of SARS-CoV-2 Virus and Diagnosis of COVID-19 Infection

Biosensors for the Determination of SARS-CoV-2 Virus and Diagnosis of COVID-19 Infection

Molecularly Imprinted Polymer-Based Sensors for SARS-CoV-2: Where Are We Now?

Eine Strep‐Tag‐geprägte Polymer‐Plattform für die heterogene Bio(elektro)katalyse

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