Peptide functionalized DNA hydrogel enhances neuroblastoma cell growth and differentiation

Hivare, Pravin; Gangrade, Ankit; Swarup, Gitanjali; Bhavsar, Krishna; Singh, Ankur; Gupta, Ratnika; Thareja, Prachi; Gupta, Sharad; Bhatia, Dhiraj

doi:10.1039/d1nr07187d

Cited by 25 publications

(21 citation statements)

References 25 publications

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“…Differentiation of the human neuroblastoma SH-SY5Y cells was performed as explained in the earlier studies. 42,43 Briefly, at day 0, when undifferentiated SH-SY5Y cells reached to 60-80% confluence, cells were trypsinized (0.25% trypsin), cell counting was done by hemocytometer, and 5 × 10 5 cells were seeded in a T-25 flask. The retinoic acid (RA) was added freshly in the differentiation media at (10 µM) final concentration.…”

Section: Differentiation Of Sh-sy5y Cells Into Neuronsmentioning

confidence: 99%

Red emitting fluorescent carbon nanoparticles to track spatio-temporal dynamics of endocytic pathways in model neuroblastoma neurons

et al. 2023

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Section: Differentiation Of Sh-sy5y Cells Into Neuronsmentioning

confidence: 99%

Red emitting fluorescent carbon nanoparticles to track spatio-temporal dynamics of endocytic pathways in model neuroblastoma neurons

et al. 2023

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“…By co-culturing both of these cells on this hydrogel, cell–cell communication was enhanced significantly, followed by the acceleration of PC12 differentiation with an increase in pseudopodia and formation of HUVECs’ tube-like structure. Hivare et al used a DNA based hydrogel that was coated on a glass and chemically conjugated with a synthetic IKVAV peptide [ 141 ]. They reported that this hydrogel triggered the enhanced differentiation of the neuronal movement of cytoskeleton and microtubules, and it prolonged neurite length, and modified endocytic mechanisms in neuroblastoma-stem cells, compared to the unmodified DNA hydrogel and controls.…”

Section: Biomedical Applications Of Peptide-based Hydrogelsmentioning

confidence: 99%

Peptide-Based Hydrogels: New Materials for Biosensing and Biomedical Applications

et al. 2022

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Peptide-based hydrogels have attracted increasing attention for biological applications and diagnostic research due to their impressive features including biocompatibility and biodegradability, injectability, mechanical stability, high water absorption capacity, and tissue-like elasticity. The aim of this review will be to present an updated report on the advancement of peptide-based hydrogels research activity in recent years in the field of anticancer drug delivery, antimicrobial and wound healing materials, 3D bioprinting and tissue engineering, and vaccines. Additionally, the biosensing applications of this key group of hydrogels will be discussed mainly focusing the attention on cancer detection.

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“…Peptides derived from proteins can become important in the selective regulation of biological systems such as cell adhesions, proliferations, and differentiations . Nonetheless, many short linear peptides have limitations in achieving sufficient bioactivity due to proteolytic degradations and rapid internalizations into the cytosol without sufficient stimulation of membrane surface receptors .…”

Section: Introductionmentioning

confidence: 99%

Two-Dimensional Peptide Assembly via Arene–Perfluoroarene Interactions for Proliferation and Differentiation of Myoblasts

et al. 2023

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Supramolecular assembly based on aromatic interactions can provide well-defined nanostructures with an understanding of intermolecular interactions at the molecular level. The peptide assembly via a supramolecular approach can overcome the inherent limitations of bioactive peptides, such as proteolytic degradations and rapid internalizations into the cytosol. Although extensive research has been carried out on supramolecular peptide materials with a two-dimensional (2D) structure, more needs to be reported on biological activity studies using well-defined 2D peptide materials. Physical and chemical properties of the 2D peptide assembly attributed to their large surface area and flexibility can show low cytotoxicity, enhanced molecular loading, and higher bioconjugation efficiency in biological applications. Here, we report supramolecular 2D materials based on the pyrene-grafted amphiphilic peptide, which contains a peptide sequence (Asp-Gly-Glu-Ala; DGEA) that is reported to bind to the integrin α2β1 receptor in 2D cell membranes. The addition of octafluoronaphthalene (OFN) to the pyrene-grafted peptide could induce a well-ordered 2D assembly by face-centered arene–perfluoroarene stacking. The DGEA-peptide 2D assembly with a flat structure, structural stability against enzymatic degradations, and a larger size can enhance the proliferation and differentiation of muscle cells via continuous interactions with cell membrane receptors integrin α2β1 showing a low intracellular uptake (15%) compared to that (62%) of the vesicular peptide assembly. These supramolecular approaches via the arene–perfluoroarene interaction provide a strategy to fabricate well-defined 2D peptide materials with an understanding of assembly at the molecular level for the next-generation peptide materials.

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Peptide functionalized DNA hydrogel enhances neuroblastoma cell growth and differentiation

Abstract: Designing programmable biomaterials that could act as extracellular matrix and permits functionalization is a current need for tissue engineering advancement. DNA based hydrogels are getting significant attention owing to their...

Cited by 25 publications

References 25 publications

Red emitting fluorescent carbon nanoparticles to track spatio-temporal dynamics of endocytic pathways in model neuroblastoma neurons

Red emitting fluorescent carbon nanoparticles to track spatio-temporal dynamics of endocytic pathways in model neuroblastoma neurons

Peptide-Based Hydrogels: New Materials for Biosensing and Biomedical Applications

Two-Dimensional Peptide Assembly via Arene–Perfluoroarene Interactions for Proliferation and Differentiation of Myoblasts

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