Peptide functionalized nanoparticles for nonviral gene delivery

Abstract

Much attention has been directed toward improving nonviral gene delivery in order to replace viral vectors and overcome the barriers to clinical gene therapy. Specifically targeting nonviral gene delivery vehicles to the disease tissue can significantly improve their delivery efficiency. Targeting peptides have been developed for growth factor receptors, integrins and tumor vasculature to target cancer, neurotrophin or neurotoxin receptors aiming to give specificity for neurodegenerative disease, airway epithelial cells to target cystic fibrosis and other tissue specific cells and receptors in bone tissue, cornea and dendritic cells.These highly specific, high-affinity peptides were identified using phage display or through rational design, and were evaluated within this review for their potential and ability to improve transfection, highlighting the successful applications of this technology and discussing the features of the design that contribute to this success. It was found that effective peptide targeting of gene delivery relied both on peptide ligand performance and on nonviral vectors that exploit the appropriate intracellular trafficking mechanisms.

RGD peptide conjugated liposomal drug delivery system for enhance therapeutic efficacy in treating bone metastasis from prostate cancer

“…To date, there is preclinical evidence that RGD peptides as αvβ3 integrin antagonist can successfully block osteoclast-mediated osteolysis in animal models of bone metastases and some of them have advanced to clinic [21,23]. On the other hand, RGD peptides conjugated drug delivery system targeting αvβ3 integrin for targeted tumor theragnostics has been also well documented in animal models of primary tumors [24,25]. There is, however, lack of results concerning with therapeutic efficacy of such drug delivery system in treating bone metastases..…”

Noncovalent functionalization of mesoporous silica nanoparticles with amphiphilic peptides

“…In particular, short peptide chains have attracted a great deal of attention in recent years because of their tunable functionality, biodegradability, and relative ease of synthesis. [20][21][22] It has been shown that short peptide sequences (e.g. RGD and IL-13) can be used for targeting nanoparticles to specic cancer cell lines.…”

Absorbable, drug-loaded, extruded fiber for implantation

“…These drug-loaded particles are then loaded into the polymer solution prior to extrusion as with other types of solution extrusion. Much research has been done in the area of gene therapy to find the best nonviral way to escort genetic sequences into a cell (Khalil et al, 2006;Levine et al, 2013), and intracellular or nuclear delivery is the most challenging area of drug delivery. Normally, for drug-loaded nanoparticles, the entire nanoparticle is released from the fiber while still encapsulating the drug.…”