Peptide P3 Selected from Phage Display Screen Shows Antiviral Activity against Porcine Reproductive and Respiratory Syndrome Virus

Liu, Ke; Huan, Beili

doi:10.4172/2254-609x.100024

Cited by 3 publications

(2 citation statements)

References 35 publications

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“…For instance, porcine reproductive and respiratory syndrome is a widespread disease affecting domestic pigs and whose etiologic agent is porcine reproductive and respiratory syndrome virus (PPRSV). Using PD screening technology against PPRSV polymerase, a novel peptide (SPHIIRNHRLSK) was identified, 54 and subsequent antiviral and cytotoxic activity assays revealed it to have high antiviral activity through its ability to bind to the PRRSV polymerase. Moreover, this peptide showed a relatively low toxicity toward cells in which viral replication was inhibited.…”

Section: Viral Infectious Diseasesmentioning

confidence: 99%

“…Moreover, this peptide showed a relatively low toxicity toward cells in which viral replication was inhibited. 54 Furthermore, classical swine fever (known as pig plague) caused by classical swine fever virus is a highly contagious disease affecting both domestic and wild swine. Phage ligands against the CSFV E2 protein were screened from an f8/8 landscape PD library, and 4 E2-specific clones displaying the sequence DRATSSNA were able to inhibit CSFV replication in the cell line PK-15.…”

Section: Viral Infectious Diseasesmentioning

confidence: 99%

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Peptide Phage Display: Molecular Principles and Biomedical Applications

Zambrano-Mila

Blacio

Vispo

2020

Ther Innov Regul Sci

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Phage display (PD) is a technology based on the presentation of functional exogenous peptides on the capsid surface of bacteriophages. PD is performed by introducing a DNA sequence of interest at a specific position within a functional viral gene. In addition, peptide phage libraries are powerful tools for expressing a wide range of random peptides and for specific peptide screening. Specifically, PD applications include the analysis of binding and interactions between proteins, the identification of bioactive peptides that bind to receptors, the identification of disease-associated antigens, and the identification of cell-specific peptides. Since its emergence, PD technology has revolutionized several fields in the biological sciences, such as oncology, cell biology, and pharmacology, the innumerable applications for which will be described throughout this review.

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Section: Viral Infectious Diseasesmentioning

confidence: 99%

Section: Viral Infectious Diseasesmentioning

confidence: 99%

Peptide Phage Display: Molecular Principles and Biomedical Applications

Zambrano-Mila

Blacio

Vispo

2020

Ther Innov Regul Sci

View full text Add to dashboard Cite

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Nanomedizin auf Phagenbasis: von Sonden zu Therapeutika für eine Präzisionsmedizin

2017

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Nanomedicine is the application of nanotechnology in medicine. This review is focused on the use of both lytic and temperate bacteriophages (phages) nanoparticles in nanomedicine, in particular, in the context of developing nanoprobes for precise disease diagnosis and nano-therapeutics for targeted disease treatment. Phages as bacteria-specific viruses do not naturally infect eukaryotic cells and are not toxic to them. They not only can be genetically engineered to bear the capability of targeting nanoparticles, cells, tissues, and organs, but also can be integrated with functional abiotic nanomaterials to gain physical properties that enable disease diagnosis and treatment. Therefore, they are ideal for many applications in precision nanomedicine. This review will summarize the current use of the great diversity of phage structures in many aspects of precision nanomedicine including ultrasensitive biomarker detection, enhanced bioimaging for disease diagnosis, targeted drug and gene delivery, directed stem cell differentiation, accelerated tissue formation, effective vaccination, and nano-therapeutics for targeted disease treatment. It will also propose future directions in the area of phage-based nanomedicines as well as the state of phage-based clinical trials.

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Phage‐Enabled Nanomedicine: From Probes to Therapeutics in Precision Medicine

2017

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Both lytic and temperate bacteriophages (phages) can be applied in nanomedicine, in particular, as nanoprobes for precise disease diagnosis and nanotherapeutics for targeted disease treatment. Since phages are bacteria-specific viruses, they do not naturally infect eukaryotic cells and are not toxic to them. They can be genetically engineered to target nanoparticles, cells, tissues, and organs, and can also be modified with functional abiotic nanomaterials for disease diagnosis and treatment. This Review will summarize the current use of phage structures in many aspects of precision nanomedicine, including ultrasensitive biomarker detection, enhanced bioimaging for disease diagnosis, targeted drug and gene delivery, directed stem cell differentiation, accelerated tissue formation, effective vaccination, and nanotherapeutics for targeted disease treatment. We will also propose future directions in the area of phage-based nanomedicines, and discuss the state of phage-based clinical trials.

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Peptide P3 Selected from Phage Display Screen Shows Antiviral Activity against Porcine Reproductive and Respiratory Syndrome Virus

Cited by 3 publications

References 35 publications

Peptide Phage Display: Molecular Principles and Biomedical Applications

Peptide Phage Display: Molecular Principles and Biomedical Applications

Nanomedizin auf Phagenbasis: von Sonden zu Therapeutika für eine Präzisionsmedizin

Phage‐Enabled Nanomedicine: From Probes to Therapeutics in Precision Medicine

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