Peptídeos bioativos do plasma de &lt;i&gt;Acanthoscurria rondoniae&lt;/i&gt;.

Riciluca, Katie Cristina Takeuti

doi:10.11606/t.87.2016.tde-29082016-090342

Cited by 2 publications

(1 citation statement)

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“…The antimicrobial effects of the fraction were evaluated by liquid growth inhibition assays as Riciluca [25], using Bacillus megaterium ATCC10778, Bacillus subtilis ATCC6633, Micrococcus luteus A270, Staphylococcus aureus ATCC29213, Enterobacter cloacae β-12, Escherichia coli SBS 363, Salmonella arizonae ATCC13314, Aspergillus niger (isolated bread), Beauveria bassiana (isolated from mummified insect), Cladosporium sp (isolated bread), Candida glabrata IOC4565, Candida krusei IOC4559 and Cryptococcus neoformans var. neoformans B-350 1A.…”

Section: Antimicrobial Assaysmentioning

confidence: 99%

Limacus flavusyellow slug: bioactive molecules in the mucus

Hayashida

Júnior

2021

Preprint

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Background: Snails and slugs were used as a treatment for many health problems therefore ancient times. Since the antimicrobial resistance became a major global thread, antimicrobial peptides have been considered as a potential source for development of new drugs, especially for drug-resistant bacteria. Nowadays reports confirm that the mucous secretions have antimicrobial, antiviral and antifungal properties. Methods: The present study has the objective to characterize and evaluate antimicrobial peptides of Limacus flavus mucus. The mucus was obtained by thermal shock and submitted to RP-HPLC. Fractions were used to perform the antimicrobial activity and hemolytic assays, electrophoresis (SDS-Page Gel) and submitted to mass spectrometry (LC-MS / MS). Identification and characterization was performed by PeaksX+ software. The physicochemical parameters were evaluated with bioinformatics tools, which predicted water solubility, iso-electric point, charge net and its primary structure. Results: Three fractions were isolated from the mucus of L. flavus and presented antifungal and antibacterial activity. The mucus showed greater inhibition for filamentous fungi (Aspergillus niger), yeast (Cryptococcus neoformans), Gram positive bacteria (Bacillus subtilis, Micrococcus luteus) and Gram negative bacteria (Enterobacter cloacae). These fractions also did not show hemolytic activity for human blood cells (erythrocytes). Fractions sequences were identified and presents Mw <3kDa, WLGH, DLQW, YLRW, respectively. Conclusion: This study revealed three antimicrobial peptides of L. flavus mucus with a wide range of antimicrobial activity and its physic-chemical characterization. Keywords: Limacus flavus, mucus, slug, antimicrobial peptide, bioactive molecules, resistance, microorganisms.

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Section: Antimicrobial Assaysmentioning

confidence: 99%