Peptides from <i>Pisum sativum</i> L. enzymatic protein digest with anti‐adhesive activity against <i>Helicobacter pylori</i>: Structure–activity and inhibitory activity against BabA, SabA, HpaA and a fibronectin‐binding adhesin

Niehues, Michael; Euler, Marco; Georgi, Gilda; Mank, Marko; Stahl, Bernd; Hensel, Andreas

doi:10.1002/mnfr.201000021

Cited by 44 publications

(54 citation statements)

References 42 publications

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“…2). The 20-30% inhibition of bacterial adhesion has to be assessed as an typical indicator for blocking of single adhesins; unspecific blockers with interaction against many or most of the OMPs will typically result in inhibition rates 470% [37]. Also, the concentrations during measurement are in the millimolar range and assessed to be quite high for antibacterial treatment.…”

Section: Antiadhesive Activity Concentration Dependency Cytotoxicitmentioning

confidence: 99%

“…1) against the tissue-specific adherence of H. pylori two different assays were performed. For quantification of antiadhesive effects and for mechanistic studies an in vitro assay with AGS cells and FITC-labeled bacteria was used to quantify H. pylori adhesion by flow cytometry [37,40]. Additionally, a semi-quantitative in situ assay, based on human tissue sections, was performed to confirm results of the in vitro assay [36].…”

Section: Antiadhesive Activity Concentration Dependency Cytotoxicitmentioning

confidence: 99%

“…pylori ATCC 700824 (strain J99, identification for quality control by PCR for vacA, cacA genes) was cultivated for two or three passages to minimize the risk of phase-variable switching of OMP genes. Cultivation was performed according to Niehues et al [37].…”

Section: Bacteria and Growth Conditionsmentioning

confidence: 99%

“…Beil (Medizinische Hochschule Hannover, Germany). Cells were grown as described by Niehues et al [37].…”

Section: Cell Culturementioning

confidence: 99%

“…All other chemicals and reagents, if not specified otherwise, were purchased from Merck (Darmstadt, Germany) of analytical quality. Fresh Okra fruit extract, serving within the antiadhesion assays as the positive control [36,37] was prepared according to Lengsfeld et al [36]. NPAs 1-3, 6-8, 11, 13, 20 and 25 were synthesized and checked for purity (498%) following the procedure reported recently [2].…”

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confidence: 99%

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Antiadhesion as a functional concept for prevention of pathogens: N‐Phenylpropenoyl‐L‐amino acid amides as inhibitors of the Helicobacter pylori BabA outer membrane protein

Niehues

Stark

Keller

et al. 2011

Molecular Nutrition Food Res

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Section: Antiadhesive Activity Concentration Dependency Cytotoxicitmentioning

confidence: 99%

Section: Antiadhesive Activity Concentration Dependency Cytotoxicitmentioning

confidence: 99%

Section: Bacteria and Growth Conditionsmentioning

confidence: 99%

“…Beil (Medizinische Hochschule Hannover, Germany). Cells were grown as described by Niehues et al [37].…”

Section: Cell Culturementioning

confidence: 99%

mentioning

confidence: 99%

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Antiadhesion as a functional concept for prevention of pathogens: N‐Phenylpropenoyl‐L‐amino acid amides as inhibitors of the Helicobacter pylori BabA outer membrane protein

Niehues

Stark

Keller

et al. 2011

Molecular Nutrition Food Res

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A versatile ceramic capillary membrane reactor system for continuous enzyme‐catalyzed hydrolysis

Messner

Antink

Guo

et al. 2021

Engineering in Life Sciences

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As an alternative to classical batch processes, enzyme-catalyzed hydrolysis can also be carried out continuously. To facilitate this, a continuous ceramic capillary membrane reactor system (CCCMRS) was developed which can be operated with various proteolytic enzymes immobilized on the porous ceramic capillary membranes. This system has several advantages over common batch processes regarding stability, reproducibility and controllability and can easily be adapted to optimal reaction conditions and individual preferences. Two exemplary applications utilizing the CCCMRS were carried out and investigated in long-term stability studies. In the first application the continuous enzymatic cleavage of human IgG into the antibody fragments Fab and Fc by immobilized papain was performed. A total volume of 22 mL of 1 mg mL -1 IgG-solution was enzymatically cleaved over a period of 33.3 h. The antibody cleavage products could be detected in an SEC-HPLC over the whole process time thus indicating long-term stability of the continuous hydrolysis process. The second application investigated the continuous digestion of pea and almond protein isolates by immobilized Alcalase resulting in the generation of a large variety of different peptides. This peptide fingerprint remains constant over a long period of time enabling fractionation and thus making the peptides accessible for further bioactivity studies in sufficient quantities. The constant peptide fingerprint could be shown in the RP-HPLC analysis for all 30 samples with a total volume of 29.7 mL collected over a period of 45 h.

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Purification, identification and molecular docking of dual‐functional peptides derived from corn protein hydrolysates with antioxidant and antiadhesive activity against Helicobacter pylori

Li,

Liu,

Miao

et al. 2024

J Sci Food Agric

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BackgroundMore than 50% of the world population is infected with Helicobacter pylori (H. pylori), which is classified as group I carcinogen by the WHO.ResultsCorn protein dual‐functional peptides were identified and functionally analyzed in vitro and in silico. Two novel dual‐functional peptides were identified as Cys‐Gln‐Asp‐Val‐Pro‐Leu‐Leu (CQDVPLL, CQ7) and Thr‐Ile‐Phe‐Pro‐Gln‐Cys (TIFPQC, TI6) by Nano‐LC‐MS/MS. The anti‐adhesive activity against Helicobacter pylori of CQ7 and TI6 was 45.17±2.41% and 48.62±1.84% at 4 mg/mL, respectively. In silico prediction showed that CQ7 and TI6 had good physicochemical properties. Molecular docking demonstrated that CQ7 and TI6 could bind to the adhesions BabA and SabA by hydrophobic interactions and hydrogen bonds, preventing Helicobacter pylori infection. Moreover, CQ7 showed strong antioxidant activity due to its unique amino acid composition.ConclusionThe present study demonstrated that the identified peptides CQ7 and TI6 possess antioxidant and anti‐adhesive effects, preventing Helicobacter pylori infection as well as alleviating oxidative injury to the gastric mucosa.This article is protected by copyright. All rights reserved.

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Peptides from Pisum sativum L. enzymatic protein digest with anti‐adhesive activity against Helicobacter pylori: Structure–activity and inhibitory activity against BabA, SabA, HpaA and a fibronectin‐binding adhesin

Abstract: Bioactive peptides from pea protein could be applied as functional ingredients for protecting infants and children against infections such as H. pylori.

Cited by 44 publications

References 42 publications

Antiadhesion as a functional concept for prevention of pathogens: N‐Phenylpropenoyl‐L‐amino acid amides as inhibitors of the Helicobacter pylori BabA outer membrane protein

Antiadhesion as a functional concept for prevention of pathogens: N‐Phenylpropenoyl‐L‐amino acid amides as inhibitors of the Helicobacter pylori BabA outer membrane protein

A versatile ceramic capillary membrane reactor system for continuous enzyme‐catalyzed hydrolysis

Purification, identification and molecular docking of dual‐functional peptides derived from corn protein hydrolysates with antioxidant and antiadhesive activity against Helicobacter pylori

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