Peptides from Royal Jelly: studies on the antimicrobial activity of jelleins, jelleins analogs and synergy with temporins

Romanelli, Alessandra; Moggio, Loredana; Montella, Rosa; Campiglia, Pietro; Iannaccone, Marco; Capuano, Federico; Pedone, Carlo; Capparelli, Rosanna

doi:10.1002/psc.1316

Cited by 83 publications

(80 citation statements)

References 24 publications

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“…For example, mammalian cationic AMPs act synergistically against bacteria including antibiotic-resistant Staphylococcus aureus strains [68]. But synergistic effects have been reported across a wide range of taxa, including oysters [36], beetles [49], hemiptera [69], honeybees [39], bumblebees [43], humans [70] and plants [71]. Often, in these studies, the effect is tested against bacteria, and in some cases, the synergy is observed with regard to effectors (e.g.…”

Section: Discussionmentioning

confidence: 99%

Insect antimicrobial peptides act synergistically to inhibit a trypanosome parasite

Marxer¹,

Vollenweider²,

Schmid‐Hempel³

2016

Phil. Trans. R. Soc. B

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One contribution of 13 to a theme issue 'Evolutionary ecology of arthropod antimicrobial peptides'. The innate immune system provides protection from infection by producing essential effector molecules, such as antimicrobial peptides (AMPs) that possess broad-spectrum activity. This is also the case for bumblebees, Bombus terrestris, when infected by the trypanosome, Crithidia bombi. Furthermore, the expressed mixture of AMPs varies with host genetic background and infecting parasite strain (genotype). Here, we used the fact that clones of C. bombi can be cultivated and kept as strains in medium to test the effect of various combinations of AMPs on the growth rate of the parasite. In particular, we used pairwise combinations and a range of physiological concentrations of three AMPs, namely Abaecin, Defensin and Hymenoptaecin, synthetized from the respective genomic sequences. We found that these AMPs indeed suppress the growth of eight different strains of C. bombi, and that combinations of AMPs were typically more effective than the use of a single AMP alone. Furthermore, the most effective combinations were rarely those consisting of maximum concentrations. In addition, the AMP combination treatments revealed parasite strain specificity, such that strains varied in their sensitivity towards the same mixtures. Hence, variable expression of AMPs could be an alternative strategy to combat highly variable infections.This article is part of the themed issue 'Evolutionary ecology of arthropod antimicrobial peptides'.

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Section: Discussionmentioning

confidence: 99%

Insect antimicrobial peptides act synergistically to inhibit a trypanosome parasite

Marxer¹,

Vollenweider²,

Schmid‐Hempel³

2016

Phil. Trans. R. Soc. B

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“…Royal Jelly is a hive product rich in bioactive compounds with various biological properties and effects: antioxidative (Guo et al, 2005(Guo et al, , 2009Jamnik et al, 2007;Silici et al, 2009; DelkhosheKasmaie et al, 2014), antiaging (Inoue et al, 2003), tonic (Fossati, 1972), antimicrobial (Fontana et al, 2004;Romanelli et al, 2011), immunomodulatory (Mannoor et al, 2009), anti-inflammatory (Kohno et al, 2004(Kohno et al, , 2015, neurotrophic, hypoglicaemic, hypocholesterolaemic (Kamakura et al, 2006;Kashima et al, 2014), vasodilative (Tokunaga et al, 2004), antitumoral, anti-allergic (Okamoto et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Quality Parameters for Commercial Royal Jelly

Mureșan¹,

MÄƒrghitaÈ²,

Dezmirean³

et al. 2016

BUASVMCN-ASB

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Royal jelly has become a high-value commercial product and the standardization of this product is required to guarantee its quality on the market. The objective of the research activity was to pursue the chemical composition of commercial samples of Royal Jelly in Romania in order to propose standardization for this product. The physicochemical composition of commercial Royal Jelly samples was analysed by determining quality parameters like: carbohydrates, lipids, proteins, 10-hydroxy-2-decenoic acid (10-HDA) and mineral elements. Carbohydrates analysis showed values between 3.4 % and 5.87 % for fructose, 4.12 % and 7.05 % for glucose, while for sucrose the values ranged between 0.95 % and 2.56 % (determined by HPLC-RI). The lipids content ranged between 1.85 % and 6.32 % (determined by the Soxhlet method). The protein values extended from 13.10 % (RJ2) to 17.04 % (RJ10) (the total protein content was determined by the Kjeldahl method). The values for the major fatty acid in Royal Jelly, 10-HDA, ranged between 1.35 % (RJ8) and 2.03 % (RJ10) (determined by high-performance liquid chromatography). The concentration of minerals varied between 3188.70 mg/kg and 4023.39 mg/kg (the concentration of minerals was measured using flame atomic absorption spectrometry). Potassium, followed by magnesium, sodium and calcium, occurs in the highest concentrations. The commercial Royal Jelly samples analysed presented variable physico-chemical characteristics that correspond with the values given by international quality standard proposals for Royal Jelly.

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“…One potential solution to the problems of both resistance and toxicity is to use a synergistic combination of antimicrobial compounds, an approach that is ubiquitous in anticancer therapy and is receiving increasing attention in the treatment of infectious diseases (2). Many species produce AMPs with known synergistic interactions, including bacteria (14), insects (20), amphibians (16,31), and humans (3,27,28), with synergy arising through a variety of mechanisms (2,14,29).…”

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confidence: 99%

Functional Synergy between Antimicrobial Peptoids and Peptides against Gram-Negative Bacteria

Chongsiriwatana

Wetzler

Barron

2011

Antimicrob Agents Chemother

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Antimicrobial peptides (AMPs) are integral components of innate immunity and are typically found in combinations in which they can synergize for broader-spectrum or more potent activity. Previously, we reported peptoid mimics of AMPs with potent and selective antimicrobial activity. Using checkerboard assays, we demonstrate that peptoids and AMPs can interact synergistically, with fractional inhibitory concentration indices as low as 0.16. These results strongly suggest that antimicrobial peptoids and peptides are functionally and mechanistically analogous.

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Peptides from Royal Jelly: studies on the antimicrobial activity of jelleins, jelleins analogs and synergy with temporins

Cited by 83 publications

References 24 publications

Insect antimicrobial peptides act synergistically to inhibit a trypanosome parasite

Insect antimicrobial peptides act synergistically to inhibit a trypanosome parasite

Quality Parameters for Commercial Royal Jelly

Functional Synergy between Antimicrobial Peptoids and Peptides against Gram-Negative Bacteria

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