Peptidsynthesen (V)

Abstract

In der Reihe der zusammenfassenden Darstellungen über die Fortschritte auf dem Gebiet der Peptidsynthese wird im folgenden ein Überblick über die Literatur von Anfang 1959 bis Mitte 1962 gegeben.

Carboxylesterases (EC 3.1.1.). The molecular weight and equivalent weight of pig liver carboxylesterase

“…While this result does not exclude dissociation of a species of mol wt 160,000 into half-molecules because of the possibility of a lowered exclusion limit for Sephadex G-100 in 8 m urea (cf'. Wieland et al, 1963;Nathans, 1965;Prfstoupil, 1965), it casts doubt on the existence of species of mol wt 42,000 in 6.5 m urea, because bovine serum albumin (mol wt 65,000) has been shown in an independent experiment to be slightly (but significantly) retarded on Sephadex G-100 in 8 m urea (0.1 m Tris, pH ~8) (A. J. Anderson and B. Zerner, unpublished results).…”

Effects of phosphate on the dissociation and enzymic stability of rabbit muscle lactate dehydrogenase

“…For this purpose the use of neutral buffers of moderate to high ionic strength (Millar, 1962; Hathaway and Griddle, 1966; Griffin and Griddle, 1970; Bartholmes et al, 1973) or of acidic conditions (Deal et al, 1963; Anderson and Weber, 1966; Jaenicke and Knof, 1968) seems plausible. Under the former conditions a rapidly established dimer-tetramer equilibrium is believed to operate, but there are also reports that dispute not only the rate of the dissociation reaction (Cho and Swaisgood, 1973) but also its existence (Wieland et al, 1963;Jaenicke and Knof, 1968; Anderson, 1969;Mire, 1969). Because of the seemingly variable dissociation behavior of different LDH preparations at neutral pH, we have explored the acidic region for suitable conditions.…”

Structure–Activity Relationships of the Adrenocorticotropins and Melanotropins: The Synthetic Approach

“…It is therefore convenient to discuss the synthesis of these fragments first. 6. Purifying all intermediates, preferably by crystallization.…”