Peptostreptococcus anaerobius: Pathogenicity, identification, and antimicrobial susceptibility. Review of monobacterial infections and addition of a case of urinary tract infection directly identified from a urine sample by MALDI-TOF MS

Legaria, María C.; Nastro, Marcela; Camporro, J.; Heger, Facundo; Barberis, Claudia; Stecher, Daniel; Rodríguez, Carlos Hernán; Vay, Carlos

doi:10.1016/j.anaerobe.2021.102461

Cited by 13 publications

(6 citation statements)

References 92 publications

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“…Palladium has two valences, +2 (most common) and +4. The electron configuration of palladium is 1s2 2s2 2p6 3s2 3p6 4s2 3d10 4p6 4d10 [7]. Like other transition elements, Palladium has an incomplete d subshell [7].…”

Section: Reactions Can Occur Due To Effective Collisionsmentioning

confidence: 99%

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Dew Formation and Anaerobiosis in Anaerobic Jars

Sudibya,

Widyaningsih

2023

AJMAH

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Introduction: This study aimed to prove whether the palladium catalyst affects the dew formation time in the anaerobic cover and whether anaerobiosis can be achieved without a palladium catalyst. Materials and Methods: This research is a laboratory experimental study with a non-randomized control group design. In this study, replication was carried out. The amount of replication used is 10. The palladium catalyst was used in 10 experiments, whereas it was not used in ten other experiments. Results: The growths of Pseudomonas aeruginosa, Clostridium tetani, and Bacteroides fragilis were observed after 48-hour incubation. The time of appearance of water condensate was observed until 24-hour incubation. The time of appearance of water condensate in the palladium-contained anaerobic jar varied between 1.37 minutes and 3.33 minutes. On the other hand, water condensate did not appear in the without-palladium anaerobic jar. No growth of Pseudomonas aeruginosa was observed in the palladium-contained anaerobic jar; on the contrary, there were ten growths in the anaerobic jar without palladium. Eight growths of Clostridium tetani were observed in the palladium-contained anaerobic jar, whereas there was no growth without palladium. No growth of Bacteroides fragilis was observed in the without-palladium anaerobic jar, whereas seven growths were observed in the anaerobic jar containing palladium. The time of appearance of water condensate in the palladium-contained anaerobic jar was different from that in the anaerobic jar without palladium. Significant differences (p < 0,01) in anaerobiosis creation between the palladium-contained anaerobic jar and the without-palladium anaerobic jar were also clearly observed.

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Section: Reactions Can Occur Due To Effective Collisionsmentioning

confidence: 99%

“…Zhu & Xu, [6] wrote that the onset of dew was less than 25 minutes. According to Legaria [7], dew is a vapor that becomes water droplets. This research proves whether the palladium catalyst affects the dew formation time in the anaerobic cover and whether anaerobiosis can be achieved without a palladium catalyst.…”

Section: Introductionmentioning

confidence: 99%

Dew Formation and Anaerobiosis in Anaerobic Jars

Sudibya,

Widyaningsih

2023

AJMAH

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“…Korean scientists developed a new MALDI-TOF MS ASTA MicroIDSys system (ASTA, Suwon, Korea). Compared to 16S rRNA sequencing and the Bruker Biotyper system, the ASTA MicroIDSys showed excellent results in identifying clinically significant anaerobic bacteria such as Peptostreptococcus anaerobius [ 78 ], Clostridium difficile , Clostridium perfringens , Finegoldia magna , Parvimonas micra [ 79 ] and aerobic Mycobacterium [ 80 ]. The Autof MS 1000ASTA and MicroIDSys systems are analogous to the MALDI BioTyper, the database is based on an isolate-specific references approach, while forbioMérieux principles (e.g., Vitek MS) are based on taxonomical group-specific principles [ 81 , 82 ].…”

Section: Bacteria Identificationmentioning

confidence: 99%

“Omic” Approaches to Bacteria and Antibiotic Resistance Identification

Janiszewska

Szultka‐Młyńska

Pomastowski

et al. 2022

IJMS

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The quick and accurate identification of microorganisms and the study of resistance to antibiotics is crucial in the economic and industrial fields along with medicine. One of the fastest-growing identification methods is the spectrometric approach consisting in the matrix-assisted laser ionization/desorption using a time-of-flight analyzer (MALDI-TOF MS), which has many advantages over conventional methods for the determination of microorganisms presented. Thanks to the use of a multiomic approach in the MALDI-TOF MS analysis, it is possible to obtain a broad spectrum of data allowing the identification of microorganisms, understanding their interactions and the analysis of antibiotic resistance mechanisms. In addition, the literature data indicate the possibility of a significant reduction in the time of the sample preparation and analysis time, which will enable a faster initiation of the treatment of patients. However, it is still necessary to improve the process of identifying and supplementing the existing databases along with creating new ones. This review summarizes the use of “-omics” approaches in the MALDI TOF MS analysis, including in bacterial identification and antibiotic resistance mechanisms analysis.

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“…The genus Peptostreptococcus, which forms a distinct phylogenetic lineage with the family Peptostreptococcaceae, belonging to the order Eubacteriales, has been frequently found in faeces, urine, mouth, blood, and gastrointestinal and genitourinary tracts in humans, mice, and canines, indicating that they can be present in various clinical specimens [1][2][3][4][5][6][7][8][9]. Streptococcus anaerobius was isolated in 1905 and reclassified as the Peptostreptococcus anaerobius type species by Kluyver and van Niel in 1936 [10].…”

Section: Introductionmentioning

confidence: 99%

Peptostreptococcus equinus sp. nov., isolated from horse faeces

Jung,

Yun,

Jung

et al. 2024

International Journal of Systematic and Evolutionary Microbiology

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A Gram-stain-positive, non-spore-forming, and obligate anaerobic bacteria designated strain CBA3647T was isolated from a horse faecal sample in Jeju, Republic of Korea. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CBA3647T formed a distinct phyletic lineage from closely related species within the genus Peptostreptococcus. Based on comparative analysis of 16S rRNA gene sequences, Peptostreptococcus anaerobius ATCC 27337T is most closely related to strain CBA3647T with a 16S rRNA gene similarity of 98.31 %, while similarity to other type strains is below 98.0 %. The genomic DNA G+C content of strain CBA3647T was 30.0 mol%. The digital DNA–DNA hybridization values between strain CBA3647T and the six Peptostreptococcus species were equal to or less than 24 %. Cells were non-motile and oval-shaped cocci with catalase-positive and oxidase-negative activities. Growth occurred at 20–40 °C (optimum, 35 °C), pH 6–8 (optimum, pH 7), and in the presence of 0–2 % (w/v) NaCl (optimum, 1 %). Strain CBA3647T contained C14 : 0 iso and C16 : 0 as major fatty acids. Phenotypic, chemotaxonomic, and molecular properties of strain CBA3647T suggest that it represents a novel species in the genus Peptostreptococcus, which has been named Peptostreptococcus equinus sp. nov. The type strain is CBA3647T (=KACC 22891T= JCM 35846T).

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Peptostreptococcus anaerobius: Pathogenicity, identification, and antimicrobial susceptibility. Review of monobacterial infections and addition of a case of urinary tract infection directly identified from a urine sample by MALDI-TOF MS

Cited by 13 publications

References 92 publications

Dew Formation and Anaerobiosis in Anaerobic Jars

Dew Formation and Anaerobiosis in Anaerobic Jars

“Omic” Approaches to Bacteria and Antibiotic Resistance Identification

Peptostreptococcus equinus sp. nov., isolated from horse faeces

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