Perconditioning combined with postconditioning on kidney ischemia and reperfusion

Abstract

Purpose: To evaluate if combination of perconditioning and postconditioning provides improved renal protection compared to perconditioning alone in a model of renal reperfusion injury. Methods: Thirty rats were assigned into 6 groups: normality; sham; ischemia and reperfusion; postconditioning; perconditioning; perconditioning + postconditioning. Animals were subjected to right nephrectomy and left renal ischemia for 30 minutes. Postconditioning consisted of 3 cycles of 5 min renal perfusion followed by 5 min of renal ischemia after major ischemic period. Perconditioning consisted of 3 cycles of 5 min hindlimb ischemia followed by 5 min of hindlimb perfusion contemporaneously to renal major ischemic period. After 24 hours, kidney was harvested and blood collected to measure urea and creatinine. Results: Perconditioning obtained better values for creatinine and urea level than only postconditioning (p<0.01); performing both techniques contemporaneously had no increased results (p>0.05). Regarding tissue structure, perconditioning was the only technique to protect the glomerulus and tubules (p<0.05), while postconditioning protected only the glomerulus (p<0.05). Combination of both techniques shows no effect on glomerulus or tubules (p>0.05). Conclusions: Perconditioning had promising results on ischemia and reperfusion induced kidney injury, enhanced kidney function and protected glomerulus and tubules. There was no additive protection when postconditioning and perconditioning were combined.

Mammalian STE20-Like Kinase 1 Deletion Alleviates Renal Ischaemia-Reperfusion Injury via Modulating Mitophagy and the AMPK-YAP Signalling Pathway

“…The procedure of the I/R injury model was performed as previously described [30]. In short, I/R injury was achieved by unilateral clamping (micro aneurysm clamps) in one renal pedicle for 30 min under general inhalation anaesthesia (3% isoflurane and oxygen).…”

Remote ischemic conditioning improves rat brain antioxidant defense in a time-dependent mechanism

“…The protocol using three alternating cycles of 5 minutes of ischemia and reperfusion was chosen, because it was extensively studied in perconditioning research 1 , 26 - 29 , showing promisors results in hepatic, renal and cardiac IR. Costa et al .…”

Ischemic perconditioning on mesenteric ischemia/reperfusion injury in rats

“…However, no studies have been found yet using remote ischemic conditioning technique associated to mesenteric IR injury, in order to assess whether this technique is really capable of attenuating IR injuries in the intestine, while perconditioning was already effective in cases of myocardial 14 , cerebral 20 and renal 13 ischemia injury.…”

“…1 ). Hindlimb ischemia was achieved using an elastic rubber band tied around the thigh of the left leg 8 , 13 . The remote ischemic conditioning technique was completed at the same time as the end of the main ischemia 13 - 15 , with subsequent reperfusion of 5 minutes.…”