Perfil da composição química e atividades antibacteriana e antioxidante do óleo essencial do cravo-da-índia (Eugenia caryophyllata Thunb.)

Silvestri, Jandimara Doninelli Fior; Paroul, Natália; Czyewski, Eliane; Lerin, Lindomar Alberto; Rotava, Ieda; Cansian, Rogério Luís; Mossi, Altemir José; Toniazzo, Geciane; Oliveira, Débora de; Treichel, Helen

doi:10.1590/s0034-737x2010000500004

Cited by 75 publications

(50 citation statements)

References 18 publications

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“…Although a number of works were presented in the literature about the biological properties of clove oil and eugenol [6][7][8], a lack was found related to the evaluation of the antimicrobial and antioxidant potential of eugenol esters obtained by enzymatic catalysis for further application in food and cosmetic industries. Several works relate the synthesis of esters from acids and anhydrides by enzymatic catalysis in organic and solvent-free systems; however, just a few use eugenol as substrates [9][10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial and Antioxidant Activities of Clove Essential Oil and Eugenyl Acetate Produced by Enzymatic Esterification

Vanin

Orlando

Piazza

et al. 2014

Appl Biochem Biotechnol

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This work reports the maximization of eugenyl acetate production by esterification of essential oil of clove in a solvent-free system using Novozym 435 as catalyst. The antimicrobial and antioxidant activities of clove essential oil and eugenyl acetate produced were determined. The conditions that maximized eugenyl acetate production were 60 °C, essential oil of clove to acetic anhydride ratio of 1:5, 150 rpm, and 10 wt% of enzyme, with a conversion of 99.87 %. A kinetic study was performed to assess the influence of substrates' molar ratio, enzyme concentration, and temperature on product yield. Results show that an excess of anhydride, enzyme concentration of 5.5 wt%, 50 °C, and essential oil of clove to acetic anhydride ratio of 1:5 afforded nearly a complete conversion after 2 h of reaction. Comparing the antibacterial activity of the essential oil of clove before and after esterification, we observed a decrease in the antimicrobial activity of eugenyl acetate, particularly with regard to minimum inhibitory concentration (MIC). Both eugenyl acetate and clove essential oil were most effective to the gram-negative than gram-positive bacteria group. The results showed a high antioxidant potential for essential oil before and particularly after the esterification reaction thus becoming an option for the formulation of new antioxidant products.

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Section: Introductionmentioning

confidence: 99%

Antimicrobial and Antioxidant Activities of Clove Essential Oil and Eugenyl Acetate Produced by Enzymatic Esterification

Vanin

Orlando

Piazza

et al. 2014

Appl Biochem Biotechnol

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“…This phenylpropanoid was also identified in the essential oil from flowers of E. klotzschiana and has been related to its high antioxidant potential (Teixeira et al 2013, Affonso et al 2012, Silvestri et al 2010.…”

Section: Resultsmentioning

confidence: 96%

“…This result can be explained by the presence of secondary metabolites, such as sesquiterpenes and monoterpenes, including α-copaene, α-(E)-bergamotene, β-bisabolene and eugenol, which have antimicrobial activity against Gram-positive and Gram-negative human pathogens (Lang and Buchbauer 2012, Affonso et al 2012, Silvestri et al 2010. Essential oils from leaves and flowers of E. klotzschiana had good antibacterial activity against P. nigrescens and S. mutans whereas they had moderate activity against S. salivarius, S. mitis, S. sanguinis and S. sobrinus.…”

Section: Biological Activities Of Eugenia Klotzschiana 1913mentioning

confidence: 99%

Chemical composition, antioxidant and antibacterial activities of essential oils from leaves and flowers of Eugenia klotzschiana Berg (Myrtaceae)

Carneiro

Alves

et al. 2017

An. Acad. Bras. Ciênc.

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Many essential oils (EOs) of different plant species possess interesting antimicrobial effects on buccal bacteria and antioxidant properties. Eugenia klotzschiana Berg (pêra-do-cerrado, in Portuguese) is a species of Myrtaceae with restricted distribution in the Cerrado. The essential oils were extracted through the hydrodistillation technique using a modified Clevenger apparatus (2 hours) and chemically characterized by GC-MS. The major compounds were α-copaene (10.6 %) found in oil from leaves in natura, β-bisabolene (17.4 %) in the essential oil from dry leaves and α-(E)-bergamotene (29.9 %) in oil from flowers. The antioxidant activity of essential oils showed similarities in both methods under analysis (DPPH and ABTS˙+) and the results suggested moderate to high antioxidant activity. The antibacterial activity was evaluated by determining minimum inhibitory concentrations (MICs), using the microdilution method. MIC values below 400 µg/mL were obtained against Streptococcus salivarius (200 µg/mL), S. mutans (50 µg/mL), S. mitis (200 µg/mL) and Prevotella nigrescens (50 µg/mL). This is the first report of the chemical composition and antibacterial and antioxidant activities of the essential oils of E. klotzschiana. These results suggest that E. klotzschiana, a Brazilian plant, provide initial evidence of a new and alternative source of substances with medicinal interest.

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“…Furthermore, all gas chromatographic methods found in the literature and employed for this essential oil analysis are slower; hence, this is probably the fastest method developed to date (Jirovetz et al 2006, Alma et al 2007, Chaieb et al 2007, Scherer et al 2009, Hajlaoui et al 2010, Silvestri et al 2010.…”

Section: Analytical Methods Optimization Processmentioning

confidence: 99%

Development and evaluation of an inhalation chamber for in vivo tests

Silva

Bizzo

Fernandes

et al. 2017

An. Acad. Bras. Ciênc.

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The bioavailability, toxicity, and therapeutic efficacy of a drug is directly related to its administration route. The pulmonary route can be accessed by inhalation after fumigation, vaporization or nebulization. Thus, pharmacological and toxicological evaluation accessed by an apparatus specifically designed and validated for this type of administration is extremely important. Based on pre-existing models, an inhalation chamber was developed. This presents a central structure with five animal holders. The nebulized air passes directly and continuously through these holders and subsequently to an outlet. Evaluation of its operation was performed using clove essential oil, a nebulizer, and a flow meter. The air within the chamber was collected by static headspace and analyzed by gas chromatography with a flame ionization detector. For this purpose, a 2.5 minutes chromatographic method was developed. The air flow in each of the five outputs was 0.92 liters per minute. During the first minute, the chamber became saturated with the nebulized material. Homogeneous and continuous operation of the chamber was observed without accumulation of volatile material inside it for 25 minutes. The inhalation chamber works satisfactorily for in vivo tests with medicines designed to be administrated by inhalation.

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Perfil da composição química e atividades antibacteriana e antioxidante do óleo essencial do cravo-da-índia (Eugenia caryophyllata Thunb.)

Cited by 75 publications

References 18 publications

Antimicrobial and Antioxidant Activities of Clove Essential Oil and Eugenyl Acetate Produced by Enzymatic Esterification

Antimicrobial and Antioxidant Activities of Clove Essential Oil and Eugenyl Acetate Produced by Enzymatic Esterification

Chemical composition, antioxidant and antibacterial activities of essential oils from leaves and flowers of Eugenia klotzschiana Berg (Myrtaceae)

Development and evaluation of an inhalation chamber for in vivo tests

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