Perfil de suscetibilidade antimicrobiana e presença do gene vapA em Rhodococcus equi de origem humana, ambiental e equina

Girardini, Lilian Kolling; Gressler, Letícia Trevisan; Costa, Mateus Matiuzzi da; Botton, Sônia de Ávila; Pellegrini, Débora da Cruz Payão; Vargas, Agueda Castagna de

doi:10.1590/s0100-736x2013000600008

Cited by 4 publications

(1 citation statement)

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“…Different strategies have been proposed for monitoring the multidrug resistance of bacteria, particularly to conventional drugs (TAVARES, 2007). Although the antimicrobial multiple resistance index was originally used for E. coli (KRUMPERMAN, 1983), this index has recently been used for other enterobacteria, including gram-positive species (GIRARDINI et al, 2013). In this study, multidrug resistance index ≥ 0.3 was observed in 33 (45.21%) isolates, particularly from dogs (n = 25; 75.76%) with cystitis (n = 13; 52%).…”

Section: Aminoglycosidesmentioning

confidence: 71%

Antimicrobial multiple resistance index, minimum inhibitory concentrations, and extended-spectrum beta-lactamase producers of Proteus mirabilis and Proteus vulgaris strains isolated from domestic animals with various clinical manifestations of infection

Zappa¹,

Bolaños

Paula

et al. 2017

SCA

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Proteus spp. are opportunistic multidrug resistant enterobacteria associated with diverse clinical diseases in domestic animals. However, Proteus infections in domestic animals are often misdiagnosed or considered contaminants in microbiological cultures rather than a primary agent of disease. Descriptions of Proteus infections in domestic animals are typically restricted to case reports, retrospective studies, or surveillance of other microorganisms. The present study investigated multiple antibiotic resistance indices, minimum inhibitory concentrations (MICs), and ESBL production in 73 strains of Proteus mirabilis (n = 69) and Proteus vulgaris (n = 4) isolated from domestic animals with various clinical manifestations. In dogs, the pathogen was most commonly associated with cystitis (48.21), enteritis (21.42%), otitis (14.29%), and conjunctivitis (3.57%). In bovines, the microorganism was predominant in cases of enteritis (22.22%), abscess (11.11%), otitis (11.11%), omphalitis (11.11%), and peritonitis (11.11%), and in organ fragments (11.11%). In equines (50.0%) and cats (100.0%), diarrhea was the main clinical sign. In vitro standard disk diffusion assay showed that the most effective antimicrobials against the isolates were imipenem (98. novobiocin (95.89), azithromycin (57.53), and trimethropim/sulfamethoxazole (39.73). Among the 73 isolates, the efficacy of amoxicillin/clavulanic acid, gentamicin, ceftriaxone, and ciprofloxacin according to MICs was 87.67%, 86.30%, 84.93%, and 82.19%, respectively. The MIC 50 values of amoxicillin/clavulanic acid, ceftriaxone, ciprofloxacin, and gentamicin were, respectively, 1.0, 0.004, 0.03, and 1.0 µg/mL. Thirty-three strains (45.21%) showed a antimicrobial multiple resistance index of ≥0.3. Multidrug resistance profiles of isolates were observed most frequently in dogs (n = 25; 75.76%), particularly in those with cystitis (n = 13; 52.0%), followed by bovines (n = 4; 12.12%), equines (n = 2; 6.06%), and cats (n = 2; 6.06%). Two (2.7%) strains, obtained from canine skin and feces, were diagnosed phenotypically as ESBL-producers. Here, we observed the diversity of Proteus infections in domestic animals. The presence of multidrug-resistant isolates and ESBL-producers reinforces the need for appropriate antimicrobial use and in vitro antimicrobial tests to support therapy. Key words: Proteus sp. Multidrug resistant. Extended-spectrum beta-lactamase. Livestock. Companion animals. ResumoProteus spp. são enterobactérias oportunistas, multirresistentes aos antimicrobianos, associadas a diversas infecções em animais domésticos. No entanto, as infecções por Proteus em animais de produção e de companhia são negligenciadas ou, por vezes, o patógeno é considerado "contaminante", ainda que em infecções como agente primário. Os registros de infecções por Proteus sp. em animais domésticos estão restritos aos relatos de casos, estudos retrospectivos ou compondo estudos com outros microorganismos. O presente estudo investigou o índice de resistência múltipla (IRMA) e a concentra...

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Section: Aminoglycosidesmentioning

confidence: 71%