Perfluorocarbons: New Tool for Islets Preservation In Vitro

Maillard, Élisa; Sánchez-Domínguez, Margarita; Kleiss, Charlotte; Langlois, A.; Sencier, Marie-Christine; Vodouhe, C.; Beitigier, W.; Krafft, Marie Pierre; Pinget, M.; Belcourt, A.; Sigrist, S.

doi:10.1016/j.transproceed.2008.01.006

Cited by 16 publications

(13 citation statements)

References 7 publications

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“…We have previously described our liquid-liquid interface culture system, and now show that it has beneficial proved viability and secretory function (18,21).…”

Section: Discussionmentioning

confidence: 99%

Use of Perfluorodecalin for Pancreatic Islet Culture Prior to Transplantation: A Liquid-Liquid Interface Culture System—Preliminary Report

et al. 2011

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Although the issue remains controversial, short-term culture is probably beneficial for islet graft quality. However, significant islet loss is invariably observed. This is related to reduced survival of large islets, which is compromised by hypoxia under standard culture conditions. We aimed to develop a method of culture, which would avoid exposure to relative hypoxia and hence maintain the quality of islets. Isolated rat islets cultured for 48 h in a liquid-liquid interface culture system (LICS) with a perfluorocarbon were compared to islets cultured under standard (C1) and suboptimal conditions (C2). Islets were tested for viability and response to a glucose challenge, and a marginal mass was transplanted into syngeneic diabetic recipients. The viability of islets after 24-h culture in LICS was higher than in C1 and C2 groups (89.0% vs. 77.5% and 64.6%, respectively) and decreased with time to reach 79.0%, 62.9%, and 53.4% after 72-h culture. The stimulation index in LICS-cultured islets was also significantly higher than in C1 and C2 groups (12.3 ± 0.4 vs. 5.8 ± 0.5 and 4.1 ± 0.2, respectively). Following transplantation of LICS-cultured islets 50% of recipients were rendered normoglycemic compared with 14.3% and 31.3% for C2 and fresh islets, respectively. Our liquid-liquid interface culture system using perfluorodecalin provides optimized culture conditions, which preserve both islet viability and their ability to engraft successfully after intraportal transplantation and could be used for islet transportation.

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“…We have previously described our liquid-liquid interface culture system, and now show that it has beneficial proved viability and secretory function (18,21).…”

Section: Discussionmentioning

confidence: 99%

Use of Perfluorodecalin for Pancreatic Islet Culture Prior to Transplantation: A Liquid-Liquid Interface Culture System—Preliminary Report

et al. 2011

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“…[6, 10–12] In particular, PFAS have been shown to preserve pancreatic islet viability, functionality, and extracellular matrix in murine models. [13] They have also been shown to increase insulin mRNA expression[13] and to reduce pancreatic islet cell hypoxia and apoptosis. [14]…”

Section: Introductionmentioning

confidence: 99%

Perfluoroalkyl substances and beta cell deficient diabetes

Conway

Innes

Long

2016

Journal of Diabetes and its Complications

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Aims Perfluoroalkyl Substances (PFAS) are synthetic hydrocarbons shown to preserve pancreatic islet cell viability and reduce islet cell hypoxia and apoptosis. We investigated the relationship of serum PFAS with diabetes, and whether this varied by diabetes type. Methods 6,460 individuals with and 60,439 without diabetes from the C8 Health Project, were categorized into three groups: Type 1 (n=820), Type 2 (n=4,291), or Uncategorized diabetes (n=1,349, missing data on diabetes type or diabetes based on blood sugar at study entry). Four PFAS were investigated: perfluorohexane sulfonate (PFHxS), perfluoroctanoic acid (PFOA), perfluoroctane sulfonate (PFOS), and perfluorononaoic acid (PFNA). Results PFAS levels were significantly lower in those with diabetes, and lowest in those with Type 1 diabetes. In age and sex adjusted analyses, ORs (CI) for Type 1, Type 2, and Uncategorized diabetes compared to no diabetes were 0.59 (0.54–0.64), 0.74 (0.71–0.77), 0.84 (0.78–0.90) respectively for PFHxS; 0.69 (0.65–0.74), 0.87 (0.89–0.91), 0.92 (0.88–0.97) respectively for PFOA; 0.65 (0.61–0.70), 0.86 (0.82–0.90), 0.93 (0.86–1.03) respectively for PFOS; and 0.65 (0.57–0.74), 0.94 (0.88–1.00), 0.95 (0.85–1.06), respectively for PFNA. Further adjustment for eGFR and other covariates did not eliminate these inverse associations. Conclusions PFAS levels were negatively associated with diabetes. This inverse relationship was strongest for Type 1 diabetes, suggesting the relationship with serum PFAS may vary with the severity of islet cell deficiency.

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“…During the TLM, PFC is insufflated with a continuous flow of oxygen (rate 50–100 cc/min to maintain partial pressure of 10–12 mmHg). Pancreata are then oxygenated via the PFC while the organ preservation solution prevents graft edema, provides substrates for metabolism, opposes the loss of purine metabolites (170, 171) and prevents deterioration of peri‐islet extracellular matrix (172).…”

Section: Two‐layered Methods Of Preservationmentioning

confidence: 99%

Preservation of the donor pancreas for whole pancreas and islet transplantation

Ridgway

Manas

Shaw

et al. 2010

Clinical Transplantation

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Whole pancreas and islet cell transplantation are both reliant upon the procurement and preservation of a high quality donor pancreas for a successful outcome. In the climate of a reducing donor pool it is imperative that donor optimization, meticulous surgical retrieval and evidence based methods of preservation are practiced to ensure optimal graft quality. Moreover expanded criteria donors and novel methods of pancreas preservation have the potential to expand the number of usable grafts and increase the availability of these transplant modalities to suitable patients with diabetes. This article provides a review of the current literature surrounding donor management, surgical technique and the various technologies of organ preservation applicable to the donor pancreas.

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Perfluorocarbons: New Tool for Islets Preservation In Vitro

Cited by 16 publications

References 7 publications

Use of Perfluorodecalin for Pancreatic Islet Culture Prior to Transplantation: A Liquid-Liquid Interface Culture System—Preliminary Report

Use of Perfluorodecalin for Pancreatic Islet Culture Prior to Transplantation: A Liquid-Liquid Interface Culture System—Preliminary Report

Perfluoroalkyl substances and beta cell deficient diabetes

Preservation of the donor pancreas for whole pancreas and islet transplantation

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