Perforin and granzyme A release as novel tool to measure NK cell activation in chickens

Ijaz, Adil; Broere, Femke; Rutten, Victor P.M.G.; Jansen, Christine A.; Veldhuizen, Edwin J.A.

doi:10.1016/j.dci.2023.105047

Cited by 3 publications

(1 citation statement)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To distinguish between NK cells and CTLs, this staining must be combined with additional mAbs to exclude degranulation of cytotoxic T cells ( 55 ) and heterophils ( 48 ). More recently, flow cytometric-based staining of the release of perforin and granzyme A was developed to measure NK cell activation in ED14 embryonic splenocytes ( 56 ), but like CD107a expression, both perforin and granzyme A are not restricted to NK cells, and multicolour analysis is warranted to exclude CTLs. The lack of exclusivity is also demonstrated by perforin and granzyme A expression in the macrophage cell line HD-11 and low levels of perforin expression in the B-cell line DT-40 ( 56 ), which is in agreement with expression in human macrophages and granzyme B secretion by human B cells.…”

Section: Natural Killer Cellsmentioning

confidence: 99%

Delineation of chicken immune markers in the era of omics and multicolor flow cytometry

Härtle,

Sutton,

Vervelde

et al. 2024

Front. Vet. Sci.

View full text Add to dashboard Cite

Multiparameter flow cytometry is a routine method in immunological studies incorporated in biomedical, veterinary, agricultural, and wildlife research and routinely used in veterinary clinical laboratories. Its use in the diagnostics of poultry diseases is still limited, but due to the continuous expansion of reagents and cost reductions, this may change in the near future. Although the structure and function of the avian immune system show commonalities with mammals, at the molecular level, there is often low homology across species. The cross-reactivity of mammalian immunological reagents is therefore low, but nevertheless, the list of reagents to study chicken immune cells is increasing. Recent improvement in multicolor antibody panels for chicken cells has resulted in more detailed analysis by flow cytometry and has allowed the discovery of novel leukocyte cell subpopulations. In this article, we present an overview of the reagents and guidance needed to perform multicolor flow cytometry using chicken samples and common pitfalls to avoid.

show abstract