Performance analysis of a reversed-phase liquid chromatographic assay of lamotrigine in plasma using solvent-demixing extraction

Traitements de l'échantillon biologique avant l'analyse chromatographique. Applications à la pharmacocinétique et à la toxicologie

“…Elle a été utilisé récemment pour le dosage des antiproteases dans le plasma de patients infectés par le virus du SIDA (29). Cette, méthode a été également utilisée pour quantifier le fluconazole, i' itraconazole et la lamotrigine dans le plasma (30)(31)(32). Il a été récemment publié que le processus de démixtion pouvait être accé-léré par l'utilisation d'ultrasons (33,34).…”

Determination of lamotrigine in small volumes of plasma by high-performance liquid chromatography

“…Several high-performance liquid chromatography (HPLC) methods were used for the determination of lamotrigine in biological fluids [7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25]. In general, the previously described methods require the use of relatively large sample volume (0.2-1 ml), and have a limit of quantitation (LOQ) of 0.2-1 g/ml.…”

Comparison of high-resolution gas chromatography and high-performance liquid chromatography for simultaneous determination of lamotrigine and carbamazepine in plasma

“…Lamotrigine has been determined in biological fluids by radioimmunoassay [12], immunofluorometry [13] , capillary electrophoresis [14], gas chromatography [15,16], but the extensively used methods are HPLC procedures using normal phase [17 20], and reverse-phase, octadecyl RP-18 [21 26], RP18 ADS (alkyl-diol-silica) [27], octyl C8 [28 31], cyanopropyl CN [32,33], and diphenyl [34] columns.…”

“…The pretreatment of biological fluids (serum or plasma) has been realized by The efficacy of LTG as an add-on therapy to existing AED regimens is well established in adult patients [2 4] and in cl children [5] [18,19,22,29], chloroform [24], acetonitrile [33], methylene chloride [20], butylacetate [15], and solid-phase extraction, C8 Bond Elut [21,28], C8 PTFE fibrils [32] and C18 [36]. Many of these methods involve extensive sample preparation with large amounts of the biological fluids and the extraction solvents.…”