Performance and optimization studies of oil extraction from Nannochloropsis spp. and Scenedesmus obliquus

Abimbola, Tobi; Christodoulatos, Christos; Lawal, Adeniyi

doi:10.1016/j.jclepro.2021.127295

Cited by 10 publications

(9 citation statements)

References 47 publications

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“…The algal slurry, at a pH of 2.0 ± 0.1, was then hydrolyzed at 120 ± 0.5 °C in a bottom stirred autoclave reactor (purchased from Amar Equipment PVT Ltd., India) for 90 min. Using the same reactor, hexane was added to the acid-hydrolyzed slurry at a biomass/solvent ratio recommended by ref . Oil extraction was performed at 80 ± 0.5 °C with consistent mixing, and afterward, the downstream separation of the lipids was performed in a separating funnel as described in ref .…”

Section: Methodsmentioning

confidence: 99%

“…Using the same reactor, hexane was added to the acid-hydrolyzed slurry at a biomass/solvent ratio recommended by ref . Oil extraction was performed at 80 ± 0.5 °C with consistent mixing, and afterward, the downstream separation of the lipids was performed in a separating funnel as described in ref . The PEAR was recovered as a byproduct and preserved for digestion.…”

Section: Methodsmentioning

confidence: 99%

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Dark Fermentation of Whole Cells and Post-extracted Alga Residues of Scenedesmus obliquus Using Immobilized Acidogens in Calcium Alginate Beads

Abimbola,

Christodoulatos,

Lawal

2023

Energy Fuels

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In this work, dark fermentation (DF) of alga and post-extracted alga residue (PEAR) of Scenedesmus obliquus is performed in an immobilized semi-batch fermenter using heatshocked digested sludge (DS) encapsulated in calcium alginate, for the first time. Encapsulation parameters at which stable beads are formed were optimized using glucose as a model compound. Microbes encapsulated in beads formed at 2% sodium alginate produced biohydrogen with over 25% more than the yield from conventional fermenters, digesting alga, and its PEAR. Reuse of immobilized microbes was demonstrated with 60% efficiency compared to the performance of fresh microbes, while digestate after DF was recovered for further processing into other bioenergy forms, such as biomethane (a downstream process impossible with the conventional suspended fermenters). This study demonstrates a clean bioenergy approach in which the biohydrogen produced can be used as a sustainable H 2 that can be targeted toward catalytic hydrodeoxygenation of algal oil into green-diesel production in place of other environmentally unfriendly hydrogen sources, while higher bioH 2 yields make alga-to-biofuel production more economically attractive.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Dark Fermentation of Whole Cells and Post-extracted Alga Residues of Scenedesmus obliquus Using Immobilized Acidogens in Calcium Alginate Beads

Abimbola,

Christodoulatos,

Lawal

2023

Energy Fuels

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“…A detailed description of the oil extraction apparatus can be found in RoyChowdhury et al ( 2019) and Abimbola et al (2021). For this study, a constant amount of ethanol (100 g solvent), 15 g of algal paste at 7.5 wt.% TS, and an extraction time of 3 h were used.…”

Section: Extraction and Quantitative Analysis Of Algal Oilmentioning

confidence: 99%

“…FAMEs analysis was performed as described by Abimbola et al (2021) following two steps. The first one corresponds to the transesterification of the lipids performed at 100 • C for 90 min, and the second step corresponds to the identification and quantification of FAME using a gas chromatograph (GC).…”

Section: Fatty Acid Methyl Esters (Fames)mentioning

confidence: 99%

Integrating biological and chemical CO2 sequestration using green microalgae for bioproducts generation

Abraham¹,

Prigiobbe²,

Abimbola³

et al. 2023

Front. Clim.

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Microalgae cultivation is considered an attractive negative emission technology (NET) due to its ability to remove carbon dioxide (CO2) from the atmosphere as well as from flue gases. Moreover, some microalgae can uptake dissolved carbon in the form of bicarbonate (HCO3-) and grow well by removing nutrients from various wastewater effluents without competing with freshwater resources. Conventional carbon mineralization is another NET where carbon is fixed into carbonate minerals such as calcite (CaCO3), magnesite (MgCO3), nesquehonite (MgCO3-3H2O), and nahcolite (NaHCO3), which can be reused in various applications such as construction and cosmetics, and in this case, to supply carbon to microalgae. Previous initial laboratory studies demonstrated the possibility of using metastable carbonates as a source of carbon for biomass growth of the freshwater microalga Scenedesmus obliquus. In this study, we present results from an experimental and modeling work where 100 L open raceway reactors were used to grow an algae polyculture using industrial nitrogen-rich wastewater. In addition, carbonates such as MgCO3-3H2O and sodium carbonate (Na2CO3) were added to the ponds to primarily buffer the pH and maintain it within the acceptable algae physiological values and to provide extra carbon to the cultures. Cultures using BG-11 growth medium and without addition of carbonates were run as controls. Continuous online monitoring of pH, temperature, and dissolved oxygen was performed while nutrients content and dry weight of algae culture were measured offline daily. A mathematical model coupled with geochemistry was implemented to describe the overall process of algae growth, carbonate dissolution, and solution composition. Biomass harvested from the raceway reactors was concentrated and oil and fatty acid methyl esters (FAME) content were determined. Results showed that biomass and oil content obtained were comparable to controls, indicating a successful metabolic-based pH control by the addition of carbonates in the ammonium-based media. Furthermore, FAME analysis revealed different profiles depending on the source of carbon used. Overall, this study suggests that integrating both types of NET strategies can contribute to the reduction of carbon emissions while producing biomass that can be further processed to generate a variety of bioproducts.

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“…Therefore, the modified Bligh & Dyer method is widely used for lipid extraction. The solvents used for lipid extraction should be cheap, non-volatile, non-toxic, and nonpolar (Abimbola et al, 2021). Before lipid extraction, the cell wall and membrane of microalgae must be disrupted.…”

Section: Lipid Extraction Technologiesmentioning

confidence: 99%

A review on biodiesel production from microalgae: Influencing parameters and recent advanced technologies

Zhang

et al. 2022

Front. Microbiol.

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Microalgae are the important part of carbon cycle in the nature, and they could utilize the carbon resource in water and soil efficiently. The abilities of microalgae to mitigate CO2 emission and produce oil with a high productivity have been proven. Hence, this third-generation biodiesel should be popularized. This review firstly introduce the basic characteristics and application fields of microalgae. Then, the influencing parameters and recent advanced technologies for the microalgae biodiesel production have been discussed. In influencing parameters for biodiesel production section, the factors of microalgae cultivation, lipid accumulation, microalgae harvesting, and lipid extraction have been summarized. In recent advanced technologies for biodiesel production section, the microalgae cultivation systems, lipid induction technologies, microalgae harvesting technologies, and lipid extraction technologies have been reviewed. This review aims to provide useful information to help future development of efficient and commercially viable technology for microalgae-based biodiesel production.

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Performance and optimization studies of oil extraction from Nannochloropsis spp. and Scenedesmus obliquus

Cited by 10 publications

References 47 publications

Dark Fermentation of Whole Cells and Post-extracted Alga Residues of Scenedesmus obliquus Using Immobilized Acidogens in Calcium Alginate Beads

Dark Fermentation of Whole Cells and Post-extracted Alga Residues of Scenedesmus obliquus Using Immobilized Acidogens in Calcium Alginate Beads

Integrating biological and chemical CO2 sequestration using green microalgae for bioproducts generation

A review on biodiesel production from microalgae: Influencing parameters and recent advanced technologies

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