In this study, the nano biochar derived from sugarcane was added with custard seed biodiesel (CBD) at different concentrations of 25 and 50 ppm to assist the emission and performance pattern of a compression ignition engine. The study monitored harmful gases, namely, hydrocarbon (HC), carbon monoxide (CO), nitrogen oxide (NO), and smoke as emission parameters and investigated brake specific fuel consumption (BSFC) and brake thermal efficiency (BTE) as performance parameters are analyzed at diverse loads by altering the concentration the biochar with CBD and compared with diesel. Experimental results revealed that the biochar addition at 50 ppm to CBD reduced smoke emission by 1.9%, CO by 0.005%, and HC by 6 ppm at all top load conditions with 71 ppm higher NO. Further, biochar addition at 50 ppm to CBD enhances the brake thermal efficiency by 0.7% and lower brake specific fuel consumption by 0.4 kg/kWh.