Performance Evaluation of a Dengue IgG Rapid Diagnostic Test Designed to Determine Dengue Serostatus as Part of Prevaccination Screening

Liberal, Vasco; Forrat, Rémi; Zhang, Cong; Pan, Charles H.; Bonaparte, Matthew; Yin, Wushan; Zheng, Lingyi; Viscardi, Valeria; Wu, Yukun; Ataman-Önal, Yasemin; Savarino, Stephen J.; Chen, Catherine

doi:10.1128/spectrum.00711-21

Cited by 7 publications

(6 citation statements)

References 22 publications

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“…Rapid diagnostic tests (RDTs) for detecting NS1 protein antigen, IgM, IgG, and IgA antibodies have been developed by many commercial companies and are widely used due to their ease of use and rapid results. In many DENV endemic settings and areas with limited laboratory diagnostic resources, RDTs provide opportunities for point-of-care diagnosis, as well as secondary infection or convalescent timepoints after recent infections [137]. The IgM-based RDT format alone is not sensitive enough for acute DF diagnosis.…”

Section: Diagnosis and Control Of Denv Infectionmentioning

confidence: 99%

Epidemiology, biology, pathogenesis, clinical manifestations, and diagnosis of dengue virus infection, and its trend in Ethiopia: a comprehensive literature review

2023

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Dengue fever is a dengue virus infection, emerging rapidly and posing public health threat worldwide, primarily in tropical and subtropical countries. Nearly half of the world's population is now at risk of contracting the dengue virus, including new countries with no previous history-like Ethiopia. However, little is known about the epidemiology and impact of the disease in different countries. This is especially true in countries, where cases have recently begun to be reported. This review aims to summarize epidemiology, biology, pathogenesis, clinical manifestations, and diagnosis of dengue virus infection and its trend in Ethiopia. It may help countries, where dengue fever is not yet on the public health list-like Ethiopia to alert healthcare workers to consider the disease for diagnosis and treatment. The review retrieved and incorporated 139 published and organizational reports showing approximately 390 million new infections. About 100 million of these infections develop the clinical features of dengue, and thousands of people die annually from severe dengue fever in 129 countries. It is caused by being bitten by a dengue virus-infected female mosquito, primarily Aedes aegypti and, lesser, Ae. albopictus. Dengue virus is a member of the Flavivirus genus of the Flaviviridae family and has four independent but antigen-related single-stranded positive-sense RNA virus serotypes. The infection is usually asymptomatic but causes illnesses ranging from mild febrile illness to fatal dengue hemorrhagic fever or shock syndrome. Diagnosis can be by detecting the virus genome using nucleic acids amplification tests or testing NS1 antigen and/or anti-dengue antibodies from serum, plasma, circulating blood cells, or other tissues. Dengue cases and outbreaks have increased in recent decades, with a significant public health impact. Ethiopia has had nearly annual outbreaks since 2013, devastating an already fragmented health system and economy. Standardization of medication, population-level screening for early diagnosis and prompt treatment, and minimization of mosquito bites reduce overall infection and mortality rates.

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Section: Diagnosis and Control Of Denv Infectionmentioning

confidence: 99%

Epidemiology, biology, pathogenesis, clinical manifestations, and diagnosis of dengue virus infection, and its trend in Ethiopia: a comprehensive literature review

2023

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“…On the contrary, the evolutionary direction of individual genes cannot be explored because of the sequences concerned in the present study were the compounded coding areas for proteins cleaved from the polyprotein. A better understanding of each coding area is also crucial for the development of rapid testing methods of viral serotype (Liberal et al, 2022 ). Meanwhile, better understanding of the seroprevalence of antibodies against dengue could be used for testing the dengue virus infections (Echegaray et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Componential usage patterns in dengue 4 viruses reveal their better evolutionary adaptation to humans

Shi

Zhang

et al. 2022

Front. Microbiol.

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There have been at least four types of dengue outbreaks in the past few years. The evolutionary characteristics of dengue viruses have aroused great concerns. The evolutionary characteristics of dengue 4 viruses are studied in the present study based on their base usage patterns and codon usage patterns. The effective number of codons and relative synonymous codon usage (RSCU) values of four types of dengue viruses were counted or calculated. The Kullback–Leibler (K–L) divergences of relative synonymous codon usage from dengue viruses to humans and the Kullback–Leibler divergences of amino acid usage patterns from dengue viruses to humans were calculated to explore the adaptation levels of dengue viruses. The results suggested that: (1) codon adaptation in dengue 4 viruses occurred through an evolutionary process from 1956 to 2021, (2) overall relative synonymous codon usage values of dengue 4 viruses showed more similarities to humans than those of other subtypes of dengue viruses, and (3) the smaller Kullback–Leibler divergence of amino acid usage and relative synonymous codon usage from dengue viruses to humans indicated that the dengue 4 viruses adapted to human hosts better. All results indicated that both mutation pressure and natural selection pressure contributed to the codon usage pattern of dengue 4 viruses more obvious than to other subtypes of dengue viruses and that the dengue 4 viruses adapted to human hosts better than other types of dengue viruses during their evolutionary process.

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“…An IgG RDT designed for use in pre-vaccination screening has been developed. It is reported as 95 % sensitive (88 % in prior monotypic infection and 98 % in multitypic infection) and 98 % specific [194]. There was no significant difference in serotype sensitivity for prior monotypic infection [197].…”

Section: Rapid Diagnostic Testsmentioning

confidence: 99%

“…Performance appeared better on serum than venous blood, and with longer assay run time, but at the expense of specificity which was reduced to 94 %. Sensitivity issues may relate to the design of these assays for detection of acute infection, with higher limits of detection [194]. Prior to using IgG acute diagnostic assays for pre-vaccination screening, local validation should be performed to ensure adequate sensitivity and specificity, as performance may relate to IgG threshold of detection for POC tests and therefore be better in regions where DENV has recently been circulating.…”

Section: Rapid Diagnostic Testsmentioning

confidence: 99%

Dengue: A review of laboratory diagnostics in the vaccine age

Frazer,

Norton

2024

Journal of Medical Microbiology

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Background. Dengue is an important arboviral infection of considerable public health significance. It occurs in a wide global belt within a variety of tropical regions. The timely laboratory diagnosis of Dengue infection is critical to inform both clinical management and an appropriate public health response. Vaccination against Dengue virus is being introduced in some areas. Discussion. Appropriate diagnostic strategies will vary between laboratories depending on the available resources and skills. Diagnostic methods available include viral culture, the serological detection of Dengue-specific antibodies in using enzyme immunoassays (EIAs), microsphere immunoassays, haemagglutination inhibition or in lateral flow point of care tests. The results of antibody tests may be influenced by prior vaccination and exposure to other flaviviruses. The detection of non-structural protein 1 in serum (NS1) has improved the early diagnosis of Dengue and is available in point-of-care assays in addition to EIAs. Direct detection of viral RNA from blood by PCR is more sensitive than NS1 antigen detection but requires molecular skills and resources. An increasing variety of isothermal nucleic acid detection methods are in development. Timing of specimen collection and choice of test is critical to optimize diagnostic accuracy. Metagenomics and the direct detection by sequencing of viral RNA from blood offers the ability to rapidly type isolates for epidemiologic purposes. Conclusion. The impact of vaccination on immune response must be recognized as it will impact test interpretation and diagnostic algorithms.

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Performance Evaluation of a Dengue IgG Rapid Diagnostic Test Designed to Determine Dengue Serostatus as Part of Prevaccination Screening

Cited by 7 publications

References 22 publications

Epidemiology, biology, pathogenesis, clinical manifestations, and diagnosis of dengue virus infection, and its trend in Ethiopia: a comprehensive literature review

Epidemiology, biology, pathogenesis, clinical manifestations, and diagnosis of dengue virus infection, and its trend in Ethiopia: a comprehensive literature review

Componential usage patterns in dengue 4 viruses reveal their better evolutionary adaptation to humans

Dengue: A review of laboratory diagnostics in the vaccine age

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